The present study was undertaken to identify potentially immunoreactive proteins of the muscle larvae (ML) and adult stage (Ad) of the nematode Trichinella spiralis Owen, 1835. To identify immunoreactive proteins that are specifically recognised by anti-Trichinella antibodies, ML and Ad crude extracts and their excretory-secretory (E-S) products were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot with serum samples from pigs experimentally infected with T. spiralis. A total of 18 bands were selected for final identification by liquid chromatography-tandem mass spectrometry. To further understand the functions of the proteins identified in this study, gene ontology terms were applied. Results showed that the specific antibodies against T. spiralis reacted with protein bands matching heat shock proteins, aminopeptidase, enolase, isocitrate dehydrogenase NADP-dependent, tropomyosin, P49 antigen, serine proteinase, secreted 5'-nucleotidase, antigen targeted by protective antibodies, 53 kDa E-S antigen, putative trypsin and paramyosin. Three proteins common for both adult stage and muscle larvae, including heat shock proteins, enolase and 5'-nucleotidase, might play important role during T. spiralis infection. These proteins are presumably presented to the host immune system and may induce humoral immune response. Thus, these proteins may be potential antigens for early diagnosis and the development of a vaccine against the parasite., Justyna Bien, Wladyslaw Cabaj, Bozena Moskwa., and Obsahuje bibliografii
a1_Neotropical freshwater stingrays (Batoidea: Potamotrygonidae) host a diversity of parasites, including some, like their hosts, that are marine-derived. Among the parasites of potamotrygonids, the cestode fauna is the most diverse, with multiple genera having been reported, including genera endemic to the freshwaters of the Neotropics and genera that have cosmopolitan distributions. Recent efforts have been made to document the diversity of cestodes of this host-parasite system and to refine the taxonomy of parasite lineages. The present study contributes to our knowledge of Rhinebothrium Linton, 1890, a diverse cosmopolitan genus of rhinebothriidean cestode, with 37 species reported from marine batoids, one species from a freshwater stingray in Borneo and six species from potamotrygonids. Rhinebothrium jaimei sp. n. is described from two species of potamotrygonids, Potamotrygon orbignyi (Castelnau) (type host) and Potamotrygon scobina Garman, from Bahía de Marajó of the lower Amazon region. It can be distinguished from most of its marine congeners via multiple attributes, including its possession of two, rather than one, posteriormost loculi on its bothridia and the lomeniform shape of its bothridium that is wider anteriorly. In addition, R. jaimei sp. n. can be distinguished from the six Rhinebothrium species described previously from potamotrygonids based on a unique combination of morphological features. Despite extensive stingray cestode sampling efforts throughout all major Neotropical river systems, we found that unlike most species of potamotrygonid Rhinebothrium species, which are widespread, R. jaimei sp. n. is restricted to the Bahía de Marajó., a2_The discovery of this new species of Rhinebothrium in Bahía de Marajó, an area in which potamotrygonids occur sympatrically with some species of euryhaline batoids (e.g. Dasyatis spp.) and share some trophic resources, suggest that modern ecological processes may be contributing to the distribution patterns of cestodes infecting potamotrygonids., Fernando P. L. Marques, Florian B. Reyda., and Obsahuje bibliografii
Historically speaking divorce is a relatively recent phenomenon. Divorce has become more important over the last century and has undoubtedly had an impact on the social reproduction strategies typically linked to marriage. In this paper use is made of the concept of Assortative Mating to explore if initial and subsequent marriage partner choices differ on the basis of heterogamy. This research question is formulated in terms of two contrasting hypotheses: (1) the learning and (2) the marriage market hypotheses (Gelissen 2004). These two perspectives form the basis of the theoretical framework used in the empirical analyses reported. Here logistic and linear regression and log-linear modelling techniques are used. The data used to test the learning and marriage market hypotheses contains information on all officially recorded marriages in the Czech Republic over a ten year period (1994-2004) gathered by the Czech Statistical Office. The results presented reveal that divorce changes the socially reproductive aspects of marriage choice strategies, and these changes vary systematically on the basis of gender. Whereas first and subsequent marriage choices are very similar for men, repeated marriage choices for women are on the whole more diverse. A number of explanations explaining this gender based difference are presented. These explanations centre on dissimilarities in the context of repeated choices for men and women.
This article brings a codicological analysis of a manuscript belonging to the Carthusian monastery at Tržek near Litomyšl that was found by researching manuscripts relating to Bohemia and held by the Biblioteca Palatina Vaticana. Moreover, the author deals with other preserved manuscripts coming from this monastery and relating to other monasteries of the same order in Bohemia and Moravia in the Middle Ages. Attention is also paid to the destiny of Albert of Sternberg, the founder of the Carthusia.
The author of this article focuses on two transcriptions of the Tovačov Book, previously unknown in the literature. The first case involves Manuscript R 4 in Strážnice Museum. The manuscript comes from the turn of the 16th and 17th centuries and is worth noting because it was transcribed from a source in a text version not too remote from that in which the Lord of Tovačov presented it to the nobles. The text is of the "Olomouc type". In addition to the Book it also provides a dual non-identical translation of Matthew's freedoms (and if we examine the other texts, the codex provides sources on Moravian provincial law up to and including the 16th century). The more recent Liberec transcription, housed in the North Bohemian Museum in Liberec, is interesting for its features which are related to manuscript A 165 of the Mitrovský collection, which we can justifiably place at the front of the Olomouc variant manuscripts' affiliation order.
The author of this article focuses on two transcriptions of the Tovačov Book, previously unknown in the literature. The first case involves Manuscript R 4 in Strážnice Museum. The manuscript comes from the turn of the 16th and 17th centuries and is worth noting because it was transcribed from a source in a text version not too remote from that in which the Lord of Tovačov presented it to the nobles. The text is of the "Olomouc type". In addition to the Book it also provides a dual non-identical translation of Matthew's freedoms (and if we examine the other texts, the codex provides sources on Moravian provincial law up to and including the 16th century). The more recent Liberec transcription, housed in the North Bohemian Museum in Liberec, is interesting for its features which are related to manuscript A 165 of the Mitrovský collection, which we can justifiably place at the front of the Olomouc variant manuscripts' affiliation order.
This article deals with the manuscript of a little known Baroque sermon called "Rurale Ivaniticum" from the Library of the Prague Crusaders. Its author is the forgotten Carmelite P. Ivanus a S. Ioanne Baptista. The main subject is the usefulness of the manuscript for the study of 18th century popular culture in Bohemia. The sermon by P. Ivanus a S. Ioanne Baptista was aimed almost exclusively at the lower class rural population. Hence the "Rurale ivaniticum" manuscript provides quite frequent examples of didactically intended folk sayings, as well as attacks on folk demonology and oneiromancy. It is from these parts of the manuscript that a merger of scholarly and folk culture clearly emerges.
This article deals with the manuscripts and incunabula which come from the Minorite Monastery in Česky Krumlov and are nowadays part of the collections of the Museum of Decorative Arts in Prague. It relates to 8 manuscripts, 6 incunabula and one paleotype, which were acquired by the Museum by purchase in the years 1894–1896, and 2 manuscripts acquired from an estate in 1961.