The basic slow Idnetics and spectral properties of the chlorophyll fluorescence temperature curve (FTC) under low actinic radíation excitation (s 2 W m"2) were measured in primary barley leaves of shade-grown plants. In contrast to the usual Fo temperature dependence, two distinct regions and two maxima of FTC were documented upon a linear heating regime. The fírst maximum situated between 49.5 and 51 °C was less sensitive, whereas Ihe position of the second maximum (between 53 and 63 “C) was strongly dependent on the heating rate. The spectral resolution of the fluorescence emission suggested a presence of photosystem (PS) 1 emission in the FTC at 436 nm excitation and an efíect of partial light-harvesting complex LHCII disconnection from the PS 2 complex at 480 nm excitation. A new fluorescence emission around 700 nm appeared upon heating. The excitation spectra in the 400 nm to 500 nm region for the 685 nm fluorescence emission wavelength indicated that only one emission form was responsible for both of the FTC bands. The 77 K fluorescence spectra at increasing, maximal and decreasing parts of Ihe second FTC band were measured using the triggering expeiiments wilh an incubation temperature of 58 oC. A disconnection of LHCI firom otiier pigment-protein complexes is suggested as a concomitant effect of Ihe second FTC maximum.
In model experíments with isolated water-soluble proteins of chloroplasts the interaction of acetylcholinesterase (AChE) with ribulose-l,5-bisphosphate carboxylase (RuBPC), plastocyanin (PC), cytochrome / (cyt f) and ferredoxin (Fd) was studied. The acetylthiocholine (AThCh) hydrolysis by AChE was stimulated by other proteins by 20-200 %. Maximal effect was observed after the addition of PC. AChE itself did not affect redox capability of the electron transport carriers. The RuBPC activity was inhibited by 70 % on the AChE increase in the reaction medium. The level of inhibition was higher in the presence of the AChE inhibitors physostigmine and neostigmine, as well as the catecholamine noradrenaline. Biomediators acetylcholine (ACh), noradrenaline, adrenaline and the anticholinesterase drugs neostigmine and physostigmine slightly (by 5-20 %) inhibited the C02-fixing enzyme activity. Mutual regulation of AChE and RuBPC may exist in chloroplasts.