The current state of the taxonomy of Rubus ser. Discolores in the Czech Republic is summarized. Since 1995, when the group was treated in the Flora of the Czech Republic (Holub 1995), six new species have been recognized, some of which are also known from adjacent areas. They are described in the present study: R. austroslovacus Trávníček, R. flos-amygdalae Trávníček et Holub, R. guttiferus Trávníček et Holub, R. parthenocissus Trávníček et Holub, R. pericrispatus Holub et Trávníček and R. portae-moravicae Holub et Trávníček. The first five belong to the group of triploid species close to R. montanus Lej. and R. grabowskii Weihe, whilst R. portae-moravicae is related to R. praecox Bertol. Two additional species, originally recognized under provisional names, were found to be identical to species described earlier: R. perperus H. E. Weber and R. phyllostachys P. J. Mueller; the latter is also found in Slovakia. At present, 17 indigenous species, one naturalized alien (R. armeniacus Focke) and one rare garden escape (R. ulmifolius Schott) of the ser. Discolores are known to occur in the Czech Republic. Distribution data and a key for the identification of all the species are presented.