The article sets out to compare the political culture and civic participation of populations in selected regions in the Czech Republic using data from the survey 'The Influence of Region-Specific Factors on the Formation of the Political Outlook of Voters', which was conducted by the Institute of Sociology in 2000-2002. The fundamental question it poses is whether the populations in selected regions differ in terms of their patterns of political culture and level of civic participation. Almond and Verba's classic definition of types of political culture is supplemented with one more type - alienation. This leads to four quantitative variables for defining the pattern of the population's mixed political culture. The level of civic participation is defined as the number of memberships in voluntary organisations. The results show that the pattern of political culture in the selected regions is almost identical. The regions do not even differ in terms of the amount of membership in voluntary civic organisations, that is, in the level of civic participation. The article concludes that in terms of political culture and civic participation the Czech Republic is quite homogeneous and is a suitable territorial unit for comparisons of political culture.