Photon-induced absorbance changes at 830 nm (ΔA830) related to redox transformations of P700, primary electron donor of photosystem 1 (PS1), were examined in barley leaves treated with diuron and methyl viologen. In such leaves, only soluble reductants localized in chloroplast stroma could serve as electron donors for P700+. Δ A830 were induced by 1-min irradiation of leaves with "actinic light" (AL, 700±6 nm) of various irradiances. Two exponentially decaying components with half-times of 2.75 (fast component, relative magnitude of 62 % of ΔA830) and 11.90 s (slow one, 38 % of ΔA830) were distinguished in the kinetics of dark relaxation of ΔA830 after leaf irradiation with saturating AL. The components reflecting P700+ dark reduction in two units of PS1 differed in the rate of electron input from stromal reductants. The decline in AL irradiance reduced steady state δA830 magnitude, which was also accompanied by a decrease in the contribution of fast component to the overall P700+ dark reduction kinetics. The photon-response curves were obtained separately for rapidly and slowly decaying δA830. The values of half-saturating irradiance were 0.106 and 0.035 μmol m-2 s-1 for rapidly and slowly reduced PS1 units, respectively. The ratio of rate constants of P700+ dark reduction for rapidly and slowly reduced PS1 units was 1.4 times higher than the ratio of their half-saturating irradiances thus indicating higher relative antenna size in rapidly reduced PS1 units. The latter finding, taken together with higher relative amount of P700, favours the view that rapidly and slowly reduced PS1 units reflect P700+ reduction by stromal reductants in spatially separated PS1α and PS1β complexes. and E. A. Egorova, N. G. Bukhov.
A theory of spectra and excitation dynamics in antenna based on the notion of exciton interactions in the cyclic structures of light-harvesting pigments is reported. The theory provides an explanation for the picosecond absorbance difference spectra, the induced absorption anisotropy decay, the anomalously high bleaching valné, as well as for the fluorescence spectra, kinetics and depolarizatio
We have studied the height dependent characteristics of the Evershed flow in the photosphere, chromosphere and chromosphere-corona transition region. We have used the Multichannel Subtractive Double Pass Spectrograph (MSDP) to
obtain line of sight velocity maps in Hα, the Meudon magnetograph for mapping the photospheric velocity field and the Ultraviolet Spectrometer and Polarimeter (UVSP) on the Solar Maximum Mission (SMM) spacecraft to obtain line of sight velocities in C IV, in sunspot regions. Our emphasis was on the large scale,
quasi-stationary characteristics of the flow. In the photosphere the velocity is low above the umbra and shows a maximum above the penumbra. In addition to the reversal of the flow in the chromosphere both the characteristic scale of the flow and the velocity are larger; the velocity maximum is located outside the photospheric penumbra. In the chromosphere-corona transition
region there is a clear tendency for a chromospheric-type Evershed effect with a predominantly horizontal flow. The velocity is greater than in the chromosphere, but the characteristic scale of the flow is about the same. In addition to the Evershed flow, C IV observations show upflows above the umbra with a velocity of about 15 km/sec.