An estimation procedure for suspended sediment concentrations based on the intensity of backscattered sound of acoustic Doppler current profilers (ADCP) is introduced in this paper. Based on detailed moving and fixed boat ADCP measurements with concurrent sediment sampling, we have successfully calibrated the estimation method for a reach of River Danube in Hungary, characterized by significant suspended sediment transport. The effect of measurement uncertainty and various data filtering on sediment load determination is also analyzed and quantified. Some of the physical model parameters describing the propagation of sound in water are estimated based on known empirical formulas, while other parameters are derived from measured. Regression analysis is used to obtain a relationship between the intensity of backscattered sound and sediment concentrations. The empirical relationship has been then used to estimate the suspended sediment concentrations from the ADCP data collected in fixed and moving boat measurement operation mode, along verticals and path-lines, respectively. We show that while some measurement uncertainty is inherent to the acoustic Doppler principle, it is further enhanced by the complexity of the near-bottom sediment-laden flow. This uncertainty has then a significant effect on the local sediment load estimation. In turn, reasonable smoothing of raw velocity and backscatter intensity data shows insignificant impact on cross-sectional sediment load estimation.
We report the observation of two types of changes in fluorescence spectra of LHCII at 4.2 K following intense illumination of the sample with a spectrally narrow laser beam at wavelengths between 678 and 686 nm. Nonspecific changes (burning-wavelength independent) are characterized by two relatively broad bands: a positive one at - 678.7 nm and a negative one at - 680.8 nm. These changes reveal a -1.3-nm blue shift of the distribution of final emitters in LHCII, from 680.3 nm to - 679.0 nm independent of the excitation wavelength. Specific fluorescence changes (burning-wavelength dependent) are characterized by a sharp hole exactly at the burning wavelength, and positive changes directly to the shorter-and longer-wavelength side of the narrow hole. The negative changes are interpreted as zero-phonon holes, while the positive features are assigned to non-photochemical products. In the low-burning intensity experiment, in addition to the zero-phonon holes, we observed also the holes to the longer wavelength of the zero-phonon hole, which were assigned to a sum of phonon and pseudo-phonon side bands. The shapes of these extra holes are identical to the shapes of the holes revealed in the fluorescence line narrowing experiment. On the basis of the low-burning intensity experiment we estimated the upper limit of the electron-phonon coupling strength for LHCII, characterized by a Huang-Rhys factor of 1.5. and K. Gibasiewicz, M. Rutkowski, R. van Grondelle.