The authors treat a manuscript missal unknown to this day by experts, which is housed at the Scientific Library in Olomouc (M III 106). After having analysed the manuscript in the line of codicology (Š. Kohout), history of arts (J. Hrbačova), and musicology (S. Červenka), they conclude that this codex was intended for the needs of the Benedictine Convent in Opatovice nad Labem. It was created shortly after the year 1354, its illuminations are the work of a Prague workshop associated with the court of King Charles IV, which participated in the production of a series of exclusive manuscripts, e. g. Liber viaticus of the Bishop Johannes Noviforensis. The Viaticus and the missal of Opatovice are on the same line in terms of decoration. The missal was probably ordered by the then Benedictine Abbot Neplach who maintained frequent official contacts with the Bishop occupying the office of chancellor to Charles IV.