This article brings a codicological analysis of a manuscript belonging to the Carthusian monastery at Tržek near Litomyšl that was found by researching manuscripts relating to Bohemia and held by the Biblioteca Palatina Vaticana. Moreover, the author deals with other preserved manuscripts coming from this monastery and relating to other monasteries of the same order in Bohemia and Moravia in the Middle Ages. Attention is also paid to the destiny of Albert of Sternberg, the founder of the Carthusia.