Although Basel book printing had a major influence on the development of humanistic studies in the 16th century, its import into the Czech lands has not been studied so far. This study explores books printed by a famous printer Johann Froben (ca. 1460–1527), and their representation in selected Czech and Moravian libraries. Using methods of provenance research, I have identified specimens that arrived in our territory not long after their printing, paying a special attention to the titles created by people from the circle of the University of Basel. My research has shown that although Czech students rarely attended Basel University until the mid-16th century, works by Basel University scholars from Froben printing house were available in Bohemia and Moravia as early as in the second and third decade of the 16th century. Furthermore, the books printed by Froben penetrated much better into the Catholic regions of Bohemia and Moravia, more open to modern humanistic studies.