Until now, the Latin Gradual and Hymn book from the beginning of the 16th century (Kutná Hora, České muzeum stříbra, shelf-mark 88/85), failed to raise the interest of musicologists. The main aim of this article is listing the polyphonic compositions not registered by the catalogue RISM B IV, 3–4. Even if the quality of writing of these polyphonic Mss. is low, it is an important source, containing several compositions (and contrafacta) which do not appear in other Bohemian sources of the same period.