Measurements of Sorbus stomata size and density, maximal photochemical efficiency of photosystem II (Fv/Fm),
variable-to-initial fluorescence ratio (Fv/F0) and potential electron acceptor capacity (‘area’) were performed during leaf development in four parental diploid species, S. aria, S. aucuparia, S. chamaemespilus, S. torminalis, and two hybrid species, S. hazslinszkyana and S. intermedia. In fully expanded mature leaves, stomata lengths and densities were significantly larger in the shrub S. chamaemespilus than in the five tree species. The best performance of both the Fv/Fm and the Fv/F0 ratio was recorded in S. intermedia, whereas S. chamaemespilus had the highest value of ‘area’. From a physiological point of view, the results of this study showed that the photosystem II reaction centers remained intact functionally through all phenological stages of leaf expansion for all examined species of Sorbus., I. Čaňová ... [et al.]., and Obsahuje bibliografii
The major light-harvesting chlorophyll (Chl) a/b complexes of photosystem II (LHCIIb) play important roles in energy balance of thylakoid membrane. They harvest solar energy, transfer the energy to the reaction center under normal light condition and dissipate excess excitation energy under strong light condition. Many bamboo species could grow very fast even under extremely changing light conditions. In order to explain whether LHCIIb in bamboo contributes to this specific characteristic, the spectroscopic features, the capacity of forming homotrimers and structural stabilities of different isoforms (Lhcb1-3) were investigated. The apoproteins of the three isoforms of LHCIIb in bamboo are overexpressed in vitro and successfully refolded with thylakoid pigments. The sequences of Lhcb1 and Lhcb2 are similar and they are capable of forming homotrimer, while Lhcb3 lacks 10 residues in the N terminus and can not form the homotrimeric structure. The pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different reconstituted Lhcbs reveal that Lhcb3 differs strongly from Lhcb1 and Lhcb2. Lhcb3 possesses the lowest Qy transition energy and the highest thermostability. Lhcb2 is the most stable monomer under strong illumination among all the isoforms. These results suggest that in spite of small differences, different Lhcb isoforms in bamboo possess similar characteristics as those in other higher plants., Z. H. Jiang ... [et al.]., and Obsahuje bibliografii
The effect of Potato virus Y NTN (PVY) infection upon photosynthesis was analysed in transgenic Pssu-ipt tobacco overproducing endogenous cytokinins in comparison with control, nontransgenic Nicotiana tabacum plants. The course of the infection from the early to the late stage was monitored by measuring of photosynthetic gas exchange and fast chlorophyll (Chl) a fluorescence induction kinetics. Leaf photosynthesis was also analysed using Chl fluorescence imaging (Chl-FI). From the different fluorescence parameters obtained using Chl-FI, the nonphotochemical quenching (NPQ) proved to be the most useful parameter to assess the effect of PVY infection. On the other hand, Chl-FI was found to be inapplicable for any presymptomatic detection of PVY infection in tobacco. The lower accumulation of the virus was found in transgenic plants and corresponded also with the presence of visible symptoms of PVY infection. The net photosynthetic rate (PN), transpiration rate (E), and stomatal conductance (gs) significantly decreased with the progress of the infection in both control plant types and transgenic rooted plants, while transgenic grafts were much less affected. The analysis of the Chl fluorescence transient revealed higher number of silent dissipative reaction centres, higher nonphotochemical dissipation, and significantly lower performance index, PI(abs), in the healthy transgenic grafts. Chl-FI also confirmed significantly higher NPQ in transgenic grafts., P. Spoustová ... [et al.]., and Obsahuje bibliografii
Chlorophyll (Chl) a fluorescence parameters and rapid light curves of soybean [Glycine max (L.) Merrill] were measured by pulse amplitude modulation fluorometry. Measurements were taken during different stages of soybean growth under field conditions with 20% enhancement in ultraviolet-B (UV-B) radiation. Results showed that supplemental UV-B radiation decreased Chl contents by 5.5% (P=0.048), 8.7% (P=0.046), and 10.5% (P=0.005) in seedling, in branching-flowering, and in pod-setting stages, respectively. In the branching-flowering and pod-setting stages, maximum quantum yield of photosystem (PS) II photochemistry (Fv/Fm) decreased by 6.1% (P=0.001) and 3.0% (P=0.009), respectively. Supplemental UV-B radiation significantly decreased the effective quantum yield (Y). The photosynthetic capacity at light saturation (Pm) also decreased in both the seedling and branching-flowering stages by 28.9% (P=0.007) and 15.5% (P=0.041), respectively. However, Y and Pm showed no significant difference in the trefoil and pod-setting stages with and without the UV treatment. The light saturation parameter (E k) decreased by 21.1% (P=0.000) and 23.2% (P=0.029) in the trefoil and seedling stages, respectively. Moreover, the initial slope (α) decreased by 21.1% (P=0.001) in the branching-flowering stage. Nonphotochemical quenching (NPQ) in the seedling stage and photochemical quenching coefficient (qp) in the
branching-flowering stage decreased significantly under UV-B treatments. The results of the present study suggest that supplemental UV-B radiation adversely affected Chl content and electron transport activity in PSII and consequently decreased the photosynthetic efficiency of soybean plants., Z. Hu ... [et al.]., and Obsahuje bibliografii
a1_Photosynthesis is one of the most important processes in plant biology and in the development of new methodologies that allow a better understanding and characterization of the photosynthetic status of organisms, which is invaluable. Flow cytometry (FCM) is an excellent tool for measuring fluorescence and physical proprieties of particles but it has seldom been used in photosynthetic studies and thus the full extent of its potentialities, in this field of research, remains unknown. To determine the suitability of FCM in photosynthesis studies, pea plants were exposed to Paraquat and their status was analyzed during 24 h. FCM was used to evaluate the integrity (volume and internal complexity) and the relative fluorescence intensity (FL) of chloroplasts extracted from those plants. To elucidate which type of information the FL conveys, FL values were correlated with the minimum fluorescence level (F0), maximum fluorescence level (Fm) and maximum photochemical efficiency of PSII (Fv/Fm), obtained by using Pulse-Amplitude-Modulation (PAM) fluorometry. Results indicate that: (1) the biomarkers used to evaluate the structural integrity of the chloroplasts were more sensitive to Paraquat exposure than the ones related to fluorescence; (2) the variation of the chloroplast’s structure, as time progressed, pointed to a swelling and subsequent burst of the chloroplast which, in turn, compromised fluorescence emission; (3) FL presented a high and significant correlation with the Fv/Fm and to a lesser degree with Fm but not with F0; (4) pigment content did not reveal significant changes in response to Paraquat exposure and is in agreement with the proposed model, suggesting that the cause for fluorescence decrease is due to chloroplast disruption., a2_In sum, FCM proved to be an outstanding technique to evaluate chloroplastidal functional and structural status and therefore it should be regarded as a valuable asset in the field of photosynthetic research., E. Rodriguez ... [et al.]., and Obsahuje bibliografii
Geitlerinema amphibium (BA-13), mat-forming cyanobacterium from the southern Baltic Sea, was grown at three irradiances [5, 65, and 125 μmol(photon) m-2 s-1] and three temperatures (15, 22.5, and 30°C). To determine the effect of the investigated factors and their interaction on culture concentration, pigment content, and photosynthetic parameters of cyanobacterium, factorial experiments and two-way analysis of variance (ANOVA) were carried out. Both chlorophyll (Chl) a and phycobilins (PB) were influenced by the irradiance and temperature, but stronger effect was noted in the case of the former one. Chl a and PB concentration per 100 μm of filament dropped above 4-fold with the increasing irradiance. The ratios between individual carotenoids [β-carotene, zeaxanthin, and myxoxanthophyll (Myx)] and Chl a increased significantly with an increase in the irradiance. The greatest fluctuations were observed in the ratio of Myx to Chl a (above 10-fold). Thus, Myx was suggested as the main photoprotective carotenoid in G. amphibium. Based on photosynthetic light response (PI) curves, two mechanisms of photoacclimation in G. amphibium were recognized: a change of photosynthetic units (PSU) number and a change of PSU size. These two mechanisms constituted the base of significant changes in photosynthetic rate and its parameters, such as the compensation point (PC), the initial slope of photosynthetic curve (α), saturation irradiance (EK), maximal photosynthetic rate (Pmax), and dark respiration rate (RD). The greatest changes were observed in PC values (about 15-fold within the range of the factors tested). Studied parameters showed a wide range of changes, which might indicate G. amphibium ability to acclimatize well to irradiance and temperature, and indirectly might explain the successful growth of cyanobacterium in dynamically changing environmental conditions., S. Jodłowska, A. Latała., and Obsahuje bibliografii
a1_Photosynthetic gas exchange, dry mass production, water relations and inducibility of crassulacean acid metabolism (CAM) pathway as well as antioxidative protection during the C3-CAM shift were investigated in Sedum album and Sedum stoloniferum from Crassulaceae under water stress for 20 days. Leaf relative water content (RWC), leaf osmotic and water potential decreased with increasing water stress in both studied species. Significant reduction in dry matter production and leaf thickness was detected only in S. stoloniferum after 20-d water stress. Δtitratable acidity and phosphoenolpyruvate carboxylase (PEPC) activity in S. album responded to drought at early stages of stress treatment, continued to increase throughout the entire stress period and reached levels 15 times higher than those in well-watered plants. In S. stoloniferum, however, both parameters responded later and after a transient increase declined again. In S. stoloniferum, in spite of increase by drought stress, net night-time CO2 assimilation was negative resembling a C3-like pattern of gas exchange. Catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) activities increased in plants subjected to mild water stress while declined as the stress became severe. Although malondialdehyde (MDA) content was higher in drought-stressed S. stoloniferum, the increase in the concentration of hydrogen peroxide (H2O2) that may act as a signal for C3-CAM transition was higher in S. album compared with S. stoloniferum. In drought-stressed plants, SOD activity showed a clear diurnal fluctuation that was more steadily expressed in S. album. In addition, such pattern was observed for CAT only in S. album. We concluded that temporal and diurnal fluctuation patterns in the activity of antioxidant enzymes depended on duration of drought stress and was related to the mode of photosynthesis and degree of CAM induction., a2_According to our results, S. stoloniferum developed a low degree of CAM activity, e.g. CAM-cycling metabolism, under drought conditions., G. Habibi, R. Hajiboland., and Obsahuje bibliografii
The diurnal trends of gas exchange and chlorophyll fluorescence parameters in four Lycoris species (L. houdyshelii, L. aurea, L. radiata var. pumila and L. albiflora) were determined and compared with a portable photosynthesis analysis system. Our study revealed that L. houdyshelii had the lowest light compensation point (LCP), while the other three species had higher LCP (12.37-14.99 μmol m-2 s-1); L. aurea had the highest light saturation point (LSP) (1,189 μmol m-2 s-1), and L. houdyshelii and L. albiflora had lower LSP with the values being 322 and 345 μmol m-2 s-1, respectively, and L. radiata var. pumila showed the intermediate LSP. Both the species L. houdyshelii and L. albiflora exhibited a typical and obvious decline in net photosynthetic rate (PN) during midday, which was not observed in L. aurea. This indicated a possible photoinhibition in L. houdyshelii and L. albiflora as the ratio of variable to maximum fluorescence (Fv/Fm) values were higher in these two species. The minimal fluorescence (F0) values were lower in L. aurea and L. radiata var. pumila. The diurnal changes of transpiration rate (E) in all four species presented only one peak, appearing between 11:00 h or 13:00 h. By using simple correlation analyses, it was observed that the environmental factors affecting
PN were different among four species and the main factors were photosynthetic photon flux density (PPFD) and relative humidity especially for L. aurea and L. radiata. The results of studying indicated that the four species could be divided into two groups. The species L. radiata var. pumila and L. aurea were more adapted to a relatively high irradiance, and L. houdyshelii and L. albiflora could be grown in moderate-shade environment in order to scale up their growth and productivity., K. Liu ... [et al.]., and Obsahuje bibliografii
Photosynthetic parameters and leaf carbon isotope composition (δ13C) in contrasting rice genotypes in relation to supplemental nitrogen (N) application and water management during the grain-filling period were compared. The changes in stomatal conductance (gs) and ratio of intercellular to ambient CO2 mole fraction (Ci/Ca) depended on the leaf nitrogen concentration (leaf N) in both ‘Hinohikari’ (temperate japonica genotype) and ‘IR36’ (indica genotype). In ‘Hinohikari’, δ13C reflects photosynthetic gas exchange during the grain-filling period, which is indicated by the significant response of δ13C to leaf N. In contrast, in ‘IR36’ δ13C did not depend on leaf N. This varietal difference in δ13C to leaf N can be attributed to a difference in the timing of leaf senescence. In ‘IR36’, leaf N and photosynthetic parameters decreased more rapidly, indicating earlier senescence and a shorter grain-filling period in comparison with ‘Hinohikari’. The significant increase in shoot dry mass in ‘Hinohikari’ resulting from supplemental N application, compared with nonsignificant effect observed in ‘IR36’, suggests that the timing of senescence in relation to the grainfilling period has a preponderant influence on productivity., S. Shimoda., and Obsahuje bibliografii
In eukaryotic oxygenic photosynthetic organisms (both plants and algae), the maximum fluorescence is at peak P, with peak M lying much lower, or being even absent. Thus, the PSMT phase, where S is semisteady state, and T is terminal state, is replaced by a monotonous P→T fluorescence decay. In the present study, we found that dimethoate-treated wheat plant leaves showed SM transient, whereas in the case of control plants monotonous P→T fluorescence decay occured. We suggest that this was partly due to quenching of fluorescence due to [H+], responsible for P to S (T) decay in control plants (Briantais et al. 1979) being replaced by state transition (state 2 to state 1) in dimethoate-treated plants (Kaňa et al. 2012)., J. K. Pandey, R. Gopal., and Obsahuje bibliografii