In order to evaluate the effect of leaf age on enzymes of carbon and sulphur metabolism, chlorophyll (Chl) and protein contents and the activities of RuBP- carboxylase (RuBPC), ATP-sulphurylase (ATPS), and 0-acetylserinesulphydrylase (OASS) were determined in leaves of different ages from luceme (Medicago sativa L. cv. Lodi and Sewa) plants grown outdoors. The enzymes of sulphur and carbon metabolism were differently regulated throughout development of luceme leaves, as ATPS reached the highest activity at very early stages of leaf growth, when RuBPC activity was negligible. OASS activity inereased 2-fold during leaf expansion, and deereased with leaf senescence. The two cultivars showed rather similar trends of development in Chl, protein content and enzyme activities. The cultivars differed also in the absolute level of enzyme activities and other biochemical parameters.
The exciton equilibration in the light-harvesting complex of photosystem 2 at room temperature was studied with pump-probe experiments under quasi-stationary conditions. The measurements were performed with two dye lasers, which had a pulse diuation of 10 ns and a spectral bandwidth of less than 0.01 nm. The relative probe beam transmission ffom 640-690 nm was recorded at different excitation wavelengths, varied from 645 to 685 nm. Two maxima were observed at 650 nm (Chl b absorption region) and at 680 nm (Chl a absorption region). The relative probe beam transmission in the Chl a region showed a course nearly independent of the pump beam wavelengťh in contrast to a weak dependence in the Chl b region. Measurements of the relative probe beam transmission as a function of pump beam intensity revealed a dominating exciton-exciton annihilation in the Chl a absorption region. In the Chl b region exciton-exciton annihilation was only marginal.
This paper deals with the reconstruction of the now longer preserved gallery of coats of arms at Roupov Castle (District of Klatovy, Western Bohemia) based on manuscripts XVII.A.8 and XVII. E. 28 a from the Czech National Library. Information from individual manuscripts was combined to form an image of probably the largest Czech family coat of arms gallery at the end of the 16th century containing a collection of coats of arms from 270 noblemen and noblewomen. The gallery probands are Jan Nezdický of Roupov († before 1607) and his two wives – Dorota Bezdružická of Kolovraty and Benigna of Švamberk. The paper draws attention to the utilization of hitherto neglected manuscript sources for research into displays of self-awareness among the privileged classes and it attempts to show the way in which the nobility used genealogical and heraldic means for representative purposes. Not least, these manuscripts are often the only source of information on genealogical and heraldic artefacts which are no longer in existence.
This paper deals with the reconstruction of the now longer preserved gallery of coats of arms at Roupov Castle (District of Klatovy, Western Bohemia) based on manuscripts XVII.A.8 and XVII. E. 28 a from the Czech National Library. Information from individual manuscripts was combined to form an image of probably the largest Czech family coat of arms gallery at the end of the 16th century containing a collection of coats of arms from 270 noblemen and noblewomen. The gallery probands are Jan Nezdický of Roupov († before 1607) and his two wives – Dorota Bezdružická of Kolovraty and Benigna of Švamberk. The paper draws attention to the utilization of hitherto neglected manuscript sources for research into displays of self-awareness among the privileged classes and it attempts to show the way in which the nobility used genealogical and heraldic means for representative purposes. Not least, these manuscripts are often the only source of information on genealogical and heraldic artefacts which are no longer in existence.