The Wheat (Triticum aestivum) – Intermediate Wheatgrass (Thinopyrum intermedium) – Couch Grass (Elymus repens) species complex is a potential case of gene flow between crop and wild relatives in the Central European area. Understanding the genomic constitutions and evolutionary histories of both polyploid Wheatgrasses and the assessment of frequency of their hybrid formation are basic prerequisities for understanding the gene flow within the whole species complex. and Václav Mahelka.