Absorption , fluorescence, delayed emission and photoacoustic spectra were obtained for the green photosynthetic bacterium. Whole cells incorporated in fluid (culture medium, viscous solution of polyvinyl alcohol, glycol) and rigid (isotropic and stretched polyvinyl alcohol film) media were investigated. The polarized absorption spectra of the stretched polyvinyl alcohol sample showed that the Qy transition moments of chlorosomal bacteriochlorophyll с was almost parallel to the film axis. Bacteriochlorophyll (BChl) с degradation occurred in some of the samples during prolonged storage and as a result a pigment absorbing at 670 nm, which was disaggregated BChl с or/and bacteriopheophytin c, was formed. This pigment was unoriented in stretched polyvinyl alcohol. The fluorescence spectrum of native cells can be analyzed using three Gaussian components at 754, 781 and 813 pm. The first component seems to be related to BChl с aggregates and the others to BChl a complexes. The time-resolved delayed luminescence spectra showed that practically all the complexes of green bacteria exhibited delayed emission but the decay times and intensities were different for the different complexes. In the photoacoustic spectra two maxima of the chlorosomal BChl с forms were well resolved and located at 748 and 765 nm. The photoacoustic maximum at 830 nm was probably related to the reaction centre (RC). In the Soret band the largest peak of the photoacoustic spectra was observed at 473 nm which showed that carotenoids absorbing in this range were losing more excitation by heat. The BChl с aggregates attached to chlorosome rods exhibited a peak at 446 nm. The efficient thermal deactivation also showed a BChl a located in RC (peak at 846 nm) and long-wavelength BChl a antenna complexes with a deactivation peak at 884 nm.
Jan Patočka, a disciple of Husserl and Heidegger, was the most eminent Czech philosopher of the 20th century. The article focuses on his concept of practical philosophy, which is seen as a constitutive force in European modernity. As such it has critical, dynamic, and stabilising effects. In this sense it is always closely related to politics and can both motivate social change and legitimise stable political orders. The consequences of this are demonstrated in liberalism and socialism as the two major projects of modern thought.