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1602. Cauda equina syndrome and nitric oxide synthase immunoreactivity in the spinal cord of the dog
- Creator:
- Jozef Maršala, Kafka, J., Naděžda Lukáčová, Dáša Čížková, Martin Maršala, and Katsube, N.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, mícha, psi, spinal cord, dogs, cauda equina syndrome, nitric oxide synthase, 14, and 612
- Language:
- English
- Description:
- The development of the cauda equina syndrome in the dog and the involvement of spinal nitric oxide synthase immunoreactivity (NOS-IR) and catalytic nitric oxide synthase (cNOS) activity were studied in a pain model caused by multiple cauda equina constrictions. Increased NOS-IR was found two days post-constriction in neurons of the deep dorsal horn and in large, mostly bipolar neurons located in the internal basal nucleus of Cajal seen along the medial border of the dorsal horn. Concomitantly, NOS-IR was detected in small neurons close to the medioventral border of the ventral horn. High NOS-IR appeared in a dense sacral vascular body close to the Lissauer tract in S1-S3 segments. Somatic and fiber-like NOS-IR appeared at five days post-constriction in the Lissauer tract and in the lateral and medial collateral pathways arising from the Lissauer tract. Both pathways were accompanied by a dense punctate NOS immunopositive staining. Simultaneously, the internal basal nucleus of Cajal and neuropil of this nucleus exhibited high NOS-IR. A significant decrease in the number of small NOS immunoreactive somata was noted in laminae I-II of L6-S2 segments at five days post-constriction while, at the same time, the number of NOS immunoreactive neurons located in laminae VIII and IX was significantly increased. Moreover, high immunopositivity in the sacral vascular body persisted along with a highly expressed NOS-IR staining of vessels supplying the dorsal sacral gray commissure and dorsal horn in S1-S3 segments. cNOS activity, based on a radioassay of compartmentalized gray and white matter regions of lower lumbar segments and non-compartmentalized gray and white matter of S1-S3 segments, proved to be highly variable for both post-constriction periods., J. Maršala, J. Kafka, N. Lukáčová, D. Čížková, M. Maršala, N. Katsube., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1603. Caught in the cycle: economic conditions at enrollment and labor market outcomes of college graduates
- Creator:
- Bičáková, Alena, Cortes, Guido Matias, and Mazza, Jacopo
- Publisher:
- CERGE-EI
- Format:
- electronic, bez média, svazek, and 51 stran : tabulky, grafy.
- Type:
- model:monograph and TEXT
- Subject:
- Ekonomie, vysokoškolské studium, hospodářské cykly, experimentální ekonomie, college study, business cycles, experimental economics, 378.14, 338.12, 33-028.77, (048.8), 4, and 33
- Language:
- English and Czech
- Description:
- Alena Bičáková, Guido Matias Cortes, Jacopo Mazza., Částečně tištěno napříč, Obsahuje bibliografii a bibliografické odkazy, and České a anglické resumé
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1604. Cazes, J. (ed.): Encyclopedia of chromatography
- Creator:
- Haisel, D.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- chromatography
- Language:
- Multiple languages
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1605. CCD - rozšíření lidského vidění: nobelovská přednáška 8. prosince 2009
- Creator:
- Boyle, Willard S. and Gregora, Ivan
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- Smith, George, Nobelova cena (ocenění), objevy a vynálezy, fyzici, nositelé cen a vyznamenání, ceny a vyznamenání, CCD snímače, Nobel Prizes, discoveries and inventions, physicists, laureates, awards, CCD scanners, optická vlákna, optické komunikační systémy, optické kabely, digitální zpracování obrazu, přenos obrazu, optical fibres, optical communications, optical cables, digital image processing, image transmission, optický přenos, CCD čipy, 19, and 621.39
- Language:
- Czech
- Description:
- Willard S. Boyle ; přeložil Ivan Gregora. and Rubrika: Referáty
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1606. CCD revolúcia v ríši elektronického obrazu ocenená polovinou Nobelovej ceny v r. 2009
- Creator:
- Ožvold, Milan
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- Boyle, Willard Sterling, 1924-2011, Smith, George Elwood, 20.-21. století, polovodiče, fyzici, Nobelova cena (ocenění), semiconductors, physicists, Nobel Prizes, Kanada, Spojené státy americké, Canada, United States, 6, and 537
- Language:
- Czech
- Description:
- Milan Ožvold. and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1607. CCP Phenogenomics Conference 2019: abstract book: Vestec, 12-13 September 2019
- Creator:
- Ústav molekulární genetiky (Akademie věd ČR), České centrum pro fenogenomiku, and CCP Phenogenomics Conference (2019 : Vestec, Praha-západ, Česko)
- Publisher:
- Institute of Molecular Genetics CAS
- Format:
- electronic, electronic resource, remote, bez média, online zdroj, and 67 stran : ilustrace, převážně barevné, portréty
- Type:
- model:monograph and TEXT
- Subject:
- Obecná genetika. Obecná cytogenetika. Evoluce, fenogenomika, molekulární genetika, phenogenomics, molecular genetics, 575.111:575.21, 577.21, (062.534), (048.3), 2, and 575
- Language:
- English
- Description:
- Název z obálky and born digital
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1608. Cd/Fe interaction in higher plants - its consequences for the photosynthetic apparatus
- Creator:
- Siedlecka, A. and Krupa, Z.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- Calvin cycle enzymes, chlorophyll, chloroplast structure, heavy metals, photosynthesis, photosystems, plant growth, and uptake of mineral elements
- Language:
- Multiple languages
- Description:
- Cadmium is one of the most dangerous environmental pollutants, affecting, among other things, plant mineral composition. It easily interacts with iron, one of the most important elements for plant growth and metabolism. This interaction, including modifying effects of lowered or excessive Fe supply on Cd-exposed plants and its consequences for the photosynthetic apparatus is reviewed. The influence of modified Fe and Cd supply on the uptake of both metals, their distribution, plant growth, and photosynthesis is also explained. Moderate Fe excess has a beneficial influence on Cd-treated plants, resulting in more intensive growth, photosynthetic pigments accumulation, and more efficient light phase of photosynthesis. Nutrient-medium Fe deficiency increases plant susceptibility to Cd. The main open questions of Cd/Fe interaction are: (1) the strong Fe-dependency of Cd mobility within the plant, and (2) photosynthetic dark phase adaptation to Cd stress. and A. Siedlecka, Z. Krupa.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1609. Cd2+ effect on photosynthetic apparatus in Synechococcus elongatus and spinach (Spinacia oleracea L.)
- Creator:
- Nováková, M., Matějová, E., and Sofrová, D.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- cyanobacteria, heavy metals, photochemical activity, photosystem 2, proteins, and ribulose-1,5-bisphosphate carboxylase/oxygenase
- Language:
- Multiple languages
- Description:
- Thylakoid membranes (TM) of the cyanobacterium Synechococcus elongatus were exposed for 30 min to the influence of 0, 10, 100, and 1 000 mM CdCl2 (= Cd0, Cd10, Cd100, and Cd1000). Cd10 and Cd100 caused some increase in activity of photosystem 2, PS2 (H2O → DCPIP), while distinct inhibition was observed with Cd1000. We also observed a similar effect when measuring oxygen evolution (H2O → PBQ + FeCy). Chloroplasts of spinach (Spinacia oleracea L.) were incubated for 30 min with 0, 15, 30, and 60 mM CdCl2 (= Cd0, Cd15, Cd30, and Cd60). All concentrations studied inhibited the PS2 activity, the effect being stronger with increasing concentration of Cd2+. The photosynthetic oxygen evolution activity was also influenced most distinctly by the highest concentration employed, i.e. Cd60. Electrophoretic analysis of the protein composition of cyanobacterium TM showed chief changes in the molecular mass regions of Mr 29 000 and 116 000, while with spinach chloroplasts the most distinct differences were observed in the regions of Mr 15 000 and 50 000. Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activity in cyanobacterial spheroplasts still remained on the 40 % level in the case of Cd1000, but it decreased down to approx. 2.5 % in the Cd60 sample of spinach chloroplasts. and M. Nováková, E. Matějová, D. Sofrová.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
1610. CD27+ peripheral blood b-cells are a useful biodosimetric marker in vitro
- Creator:
- Zuzana Řeháková, Šinkora, J., Marcela Vlková, Doris Vokurková, Jan Österreicher, Jaroslava Vávrová, and Daniel Driák
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, klinická imunologie, radiobiologie, dozi, clinical immunology, radiobiology, B-cells, CD27+, biodosimetric marker, γ-irradiation, 14, and 612
- Language:
- English
- Description:
- The CD8+ natural killer (NK) subpopulation has recently been identified as a fast and reliable biodosimetric indicator within human peripheral blood mononuclear cells (PBMC) in vitro. In irradiated and subsequently cultivated PBMC, a decrease of the relative number of intact CD3-CD8+ lymphocytes 16 and 48 h after treatment has allowed for estimating the received dose in the range of 0 - 10 Gy and lethal/sublethal dose discrimination, respectively. Here we show that suitable biodosimeters can also be found in the peripheral blood B-cell compartment. Multiparameter flow cytometric analysis of irradiated and subsequently cultivated human PBMC revealed that both the CD27+ and CD21- B-cell subpopulations can be used as biodosimeters and the CD19+CD27+ lymphocytes have proved useful for retrospective determination of the received dose in the range of 0 - 6 Gy. In addition, several CD19+ lymphocyte subsets characterized by co-expression of CD21, CD27 and CD38 have been shown to bear biodosimetric potential, too. However, when important parameters like the original size within the CD19+ compartment, its radiation-induced changes and data variation had been taken into account, the CD27+ subpopulation proved superior to the other B-cell subpopulations and subsets. It appears that, in the dose range of 0 - 6 Gy, the relative decrease of CD27+ B lymphocytes provides more sensitive and reliable data than that of CD8+ NK-cells due mainly to lower data variation. In contrast to CD27+ B-cells, the proportions of CD27+ subpopulations of T-cells were not affected by irradiation. We have also proposed a simple experimental protocol based on full blood cultivation and three-color CD27/CD3/CD19 immuno-phenotyping as a time-saving and inexpensive approach for practical biodosimetric evaluations on simple, three-to-four color flow cytometers., Z. Řeháková, J. Šinkora, M. Vlková, D. Vokurková, J. Österreicher, J. Vávrová, D. Driák., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public