Investigation of the interactions between submerged vegetation patch and flow structure is of crucial importance for river engineering. Most of hydraulic models have been presented for fully developed flows over uniform vegetation in the laboratory conditions; however, the mentioned interactions are complex in river flows where the flow is not developed along small patch. This reveals a gap between developed and non-developed flow along the vegetation patch. This study was conducted in a gravel-bed river in the central Iran. The results reveal that the flow structure in
evolving flow (non-developed flow) along the patch resembles that in shallow mixing layer. Accordingly, a shallow mixing layer model and modified equations are combined to quantify evolving area along the patch. The evolving shallow mixing layer equations for the flow along a non-uniform vegetation patch reach a reasonable agreement with field data. However, the spreading coefficient of this model less than one was reported in literature, 0.06 and 0.12. In addition, the flow immediately downstream the vegetation patch behaves similar to a jet and is parameterized by two conventional
models, conventional logarithmic law and mixing layer theory. These models present a reasonable agreement with the
measured velocity profiles immediately downstream the patch.
The join of two graphs G and H is a graph formed from disjoint copies of G and H by connecting each vertex of G to each vertex of H. We determine the flow number of the resulting graph. More precisely, we prove that the join of two graphs admits a nowhere-zero 3-flow except for a few classes of graphs: a single vertex joined with a graph containing an isolated vertex or an odd circuit tree component, a single edge joined with a graph containing only isolated edges, a single edge plus an isolated vertex joined with a graph containing only isolated vertices, and two isolated vertices joined with exactly one isolated vertex plus some number of isolated edges.
Fluid inclusions in carbonate-dominated veinlets from selected Czech Upper Paleozoic basins display large variations in salinity (0-25 wt. % eq. NaCl/CaCl2) and smaller variations in the homogenization temperatures (41-112 °C). We suggest that the trapped fluids represent a mixture dominated by heated formation fluids., Jiří Zachariáš and Jiří Pešek., and Obsahuje bibliografii
An influence of soil drought (7 or 14 d) and 7 d recovery on changes of leaf fluorescence excitation spectra at wavelengths of 450, 520, 690, and 740 nm (F450, F520, F690, F740) for drought resistant and sensitive genotypes of triticale and maize was compared. In non-stressed plants the differences between maize and triticale were observed for F450 and F520, but not for F690 and F740. Drought caused the increase in F450, F520, and F690 and this increase was more distinct for drought sensitive genotypes. After re-hydration, chlorophyll fluorescence mostly recovered to values of control plants. Drought caused significant increase in F690/F740 but not in F450/F690 and F450/F520. For triticale, highest increase in F690/F740 was observed in the 4th and 7th leaves of resistant genotype and contrarily in maize for the sensitive one. After recovery, the F450/F520, F450/F690, and F690/F740 ratios mostly returned to values of control plants. and M. T. Grzesiak ... [et al.].
We report the observation of two types of changes in fluorescence spectra of LHCII at 4.2 K following intense illumination of the sample with a spectrally narrow laser beam at wavelengths between 678 and 686 nm. Nonspecific changes (burning-wavelength independent) are characterized by two relatively broad bands: a positive one at - 678.7 nm and a negative one at - 680.8 nm. These changes reveal a -1.3-nm blue shift of the distribution of final emitters in LHCII, from 680.3 nm to - 679.0 nm independent of the excitation wavelength. Specific fluorescence changes (burning-wavelength dependent) are characterized by a sharp hole exactly at the burning wavelength, and positive changes directly to the shorter-and longer-wavelength side of the narrow hole. The negative changes are interpreted as zero-phonon holes, while the positive features are assigned to non-photochemical products. In the low-burning intensity experiment, in addition to the zero-phonon holes, we observed also the holes to the longer wavelength of the zero-phonon hole, which were assigned to a sum of phonon and pseudo-phonon side bands. The shapes of these extra holes are identical to the shapes of the holes revealed in the fluorescence line narrowing experiment. On the basis of the low-burning intensity experiment we estimated the upper limit of the electron-phonon coupling strength for LHCII, characterized by a Huang-Rhys factor of 1.5. and K. Gibasiewicz, M. Rutkowski, R. van Grondelle.
In the pursuit of knowledge on the biological behavior of Brazilian Atlantic Forest tree species, this study evaluated the susceptibility of the light-demanding species, Schinus terebinthifolia Raddi., Pseudobombax grandiflorum (Cav.) A. Robyns and Joannesia princeps Vell., and of the shade-tolerant species, Hymenaea courbaril L. var. stilbocarpa and Lecythis pisonis Camb, to photoinhibition and acclimation capacity. These species were first cultivated under two irradiance conditions, I20 (20% direct sunlight radiation) and I100 (all-sky or direct sunlight) and then transferred from I20 to I100. The effects of the sudden increase in light radiation intensity on photosynthetic activity were then evaluated through chlorophyll (Chl) fluorescence imaging, HPLC xanthophylls analysis, and cell membrane lipid peroxidation measurements. Light-demanding species were found to present a higher photochemical efficiency and higher acclimation capacity under high light irradiance than shade-tolerant species. The higher photoinhibition tolerance observed in light-demanding species was associated to their higher capacity for photochemical dissipation and dissipation of excess excitation energy via the xanthophyll cycle, leading to a lower ROS generation. The obtained results suggested that a knowledge of acclimation capacity, by means of Chl fluorescence imaging yields, is a useful indicator of species successional grouping., L. Dos Anjos, M. A. Oliva, and K. N. Kuki., and Obsahuje bibliografii
The kinetics of bacteriochlorophyll fluorescence in intact cells of the purple nonsulfur bacterium Rhodobacter sphaeroides were measured under continuous and pulsed actinic laser diode (808 nm wavelength and maximum 2 W light power) illumination on the micro- and millisecond timescale. The fluorescence induction curve was interpreted in terms of a combination of photochemical and triplet fluorescence quenchers and was demonstrated to be a reflection of redox changes and electron carrier dynamics. By adjustment of the conditions of single and multiple turnovers of the reaction center, we obtained 11 ms-1 and 120 μs-1 for the rate constants of cytochrome c23+ detachment and cyclic electron flow, respectively. The effects of cytochrome c2 deletion and chemical treatments of the bacteria and the advantages of the fluorescence induction study on the operation of the electron transport chain in vivo were discussed., G. Sipka, M. Kis, J. L. Smart, P. Maróti., and Obsahuje bibliografické odkazy
We describe an instrument that allows the rapid measurement of fluorescence lifetime-resolved images of leaves as well as sub-cellular structures of intact plants or single cells of algae. Lifetime and intensity fluorescence images can be acquired and displayed in real time (up to 55 lifetime-resolved images per s). Our imaging technique therefore allows rapid measurements that are necessary to determine the fluorescence lifetimes at the maximum (P level) fluorescence following initial illumination during the chlorophyll (Chl) a fluorescence transient (induction) in photosynthetic organisms. We demonstrate the application of this new instrument and methodology to measurements of: (1) Arabidopsis thaliana leaves showing the effect of dehydration on the fluorescence lifetime images; (2) Zea mays leaves showing differences in the fluorescence lifetimes due to differences in the bundle sheath cells (having a higher amount of low yield photosystem 1) and the mesophyll cells (having a higher amount of high yield photosystem 2); and (3) single cells of wild type Chlamydomonas reinhardtii and its non-photochemical quenching mutant NPQ2 (where the conversion of zeaxanthin to violaxanthin is blocked), with NPQ2 showing lowered lifetime of Chl a fluorescence. In addition to the lifetime differences referred to in (1) and (2), structural dependent heterogeneities in the fluorescence lifetimes were generally observed when imaging mesophyll cells in leaves. and O. Holub ... [et al.].
We demonstrate a microfluidic system that builds emulsion droplets of water in oil in one chip and provides continuous control of fluorescein concentration in the droplet and fluorescence detection by built in optical fiber. In this fluid flow chip water is mixed with an adjustable amount of fluorescein solution and injected into oil where emulsion droplets of uniform size are formed. Fluorescence from the droplets was detected directly on the chip using optical fibers. Such miniaturized chemical laboratory is useful for applications where chemical reactions or their products can be characterized by optical way. and Demonstrujeme mikrofluidní zařízení, které na jednom čipu vytváří emulzní kapénky vody v oleji s možností plynule měnit koncentraci fluoresceinu v kapénce a detekovat ji zakomponovaným optickým vláknem. V průtočném čipu se vodná fáze mísí s řízeným množstvím roztoku fluoresceinu a vstřikuje se do olejové fáze, kde vytváří kapénky sjednotnou velikostí. Fluorescence kapének byla detekována přímo na čipu optickými vlákny. Taková miniaturizovaná chemická laboratoř nachází uplatnění v aplikacích, kde je možné optickou cestou charakterizovat chemické reakce nebo detekovat jejich produkty.