The European stag beetle, Lucanus cervus, is recognised as a flagship species for biodiversity conservation. Although the species is widely distributed across Europe declines have led to it being granted protected or endangered status in a number of countries and regarded as "near threatened" by the IUCN. The integration of genetic approaches into conservation efforts is urgently needed but has been impeded to date by the lack of appropriate genetic markers. To provide such a resource the development of the first microsatellite loci for stag beetle is described. Loci were identified using two methods (i) enriched library cloning (ELC) and (ii) Restriction enzyme Associated DNA sequencing (RAD-Seq). Inefficient microsatellite detection using the ELC method suggests that RAD-Seq, or other Next Generation Sequencing based methods, may ultimately be more cost effective for obtaining informative suites of markers for this and other coleopteran species. 18 loci were characterised by genotyping 42 UK specimens collected as prey leftover/roadkill. All loci produced unambiguous genotypes and were polymorphic. Though preliminary, estimates of genetic variability suggest UK populations may be genetically depauperate. The microsatellite loci represent a suite of genetic markers that can be applied to non-invasive population monitoring and numerous other areas of Lucanus conservation and evolutionary research., Niall J. McKeown, Deborah J. Harvey, Amy J. E. Healey, Ilze Skujina, Karen Cox, Alan C. Gange, Paul W. Shaw., and Obsahuje bibliografii
In this study, we report the development of a set of 15 polymorphic microsatellite markers for the box tree moth, Cydalima perspectalis (Walker), a highly invasive insect in Europe causing significant damage to natural and ornamental Buxus trees. The markers were characterized for four distant populations in both its native (China, two populations) and invasive ranges (Czech Republic and Turkey, one population each). The number of alleles ranged from 2 to 12. No marker significantly deviated from the Hardy-Weinberg equilibrium for all the populations sampled. These microsatellite markers are promising tools for further studies on the invasive pathways and dispersal pattern of the box tree moth in Europe., Audrey Bras, Laure Sauné, Alain Roques, Jérôme Rousselet, Marie-Anne Auger-Rozenberg., and Obsahuje bibliografii
Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle., Oddmund Kleven, Anders Endrestøl., and Obsahuje bibliografii
The longhorn crazy ant, Paratrechina longicornis (Latreille), is a ubiquitous agricultural and urban pest that has invaded most tropical and subtropical regions. Although P. longicornis has been found worldwide for more than a century, the genetic structure, origin, and invasion history of this species have not yet been extensively studied, partially because of the limited number of genetic markers currently available. In the present study, we developed 36 polymorphic microsatellite markers for P. longicornis and characterized these markers by genotyping P. longicornis workers from 74 colonies in East and Southeast Asia. All loci were polymorphic, with the number of alleles per locus ranging from 3 to 18 (8.5 on average). Extremely high levels of heterozygosity were found in all populations, suggesting that workers are invariably produced from the mating of divergent queen and male lineages. Queens and males possess non-overlapping allele size ranges at 18 loci, indicating the potential resolving power of the subset of markers in inferring the history of queen and male lineages. Genetic differentiation among three studied populations was low yet significant and may likely reflect their close association with human activities. Overall, the new microsatellite markers developed in the present study serve as a practical tool to reconstruct routes of invasion and assess the population genetics of this invasive ant.
Nebulization with saline solution, although commonly used to
alleviate respiratory symptoms, particularly in children, is often
questioned concerning its effectiveness. In this study, we
investigated the effects of isotonic saline nebulization on lung
function in 40 children (mean age of 14±1 years) suffering from
different types of airway disorders. Measurements were carried
out directly before and up to 15 min after nebulization, for six
days in a row, always on the same day time in the morning. The
children were divided into two study groups according to the
baseline ratio of forced expired volume in one second/forced vital
capacity (FEV1/FVC), below and above 80 %. We found
significant improvements after saline nebulization in FEV1, midexpiratory flow at 50 % and 75 % of FVC (MEF50 and MEF75),
and peak expiratory flow (PEF) in the group with the baseline
FEV1/FVC less than 80 %. In contradistinction, children with an
index greater than 80 % displayed no appreciable changes in the
lung function variables when compared with the baseline level
before saline nebulization. We conclude that isotonic saline
nebulization might mitigate the functional signs of threatening
pulmonary obstruction and as such may be clinically useful in
pediatric patients with mild respiratory problems.
Itch is the most common chief complaint in patients visiting
dermatology clinics and is analogous to cough and also sneeze of
the lower and upper respiratory tract, all three of which are host
actions trying to clear noxious stimuli. The pathomechanisms of
these symptoms are not completely determined. The itch can
originate from a variety of etiologies. Itch originates following the
activation of peripheral sensory nerve endings following damage
or exposure to inflammatory mediators. More than one sensory
nerve subtype is thought to subserve pruriceptive itch which
includes both unmyelinated C-fibers and thinly myelinated Aδ
nerve fibers. There are a lot of mediators capable of stimulating
these afferent nerves leading to itch. Cough and itch pathways are
mediated by small-diameter sensory fibers. These cough and itch
sensory fibers release neuropeptides upon activation, which leads
to inflammation of the nerves. The inflammation is involved in the
development of chronic conditions of itch and cough. The aim of
this review is to point out the role of sensory nerves in the
pathogenesis of cough and itching. The common aspects of itch
and cough could lead to new thoughts and perspectives in both
fields.
The Global Navigation Satellite System (GNSS) can provide the daily position time series for the geodesy and geophysical studies. However, due to various unpredictable factors, such as receiver failure or bad observation conditions, missing data inevitably exist in GNSS position time series. Most traditional time series analysis methods require the time series should be completed. Therefore, filling the missing data is a valuable step before analyzing the GNSS time series. In this study, a new method named Iteration Empirical Mode Decomposition (Iteration EMD) is proposed to fill the missing data in GNSS position time series. The simulation experiments are performed by randomly removing different missing percentages of the synthetic time series, with the added different types noise. The results show that Iteration EMD approach performs well regardless of high or low missing percentage. When the missing percentage increases from 5 % to 30 % with a step of 5 %, all the Root Mean Square Errors (RMSE) and Mean Absolute Errors (MAE) of Iteration EMD are smaller than Interpolation EMD. The relative improvements at different percentages of Iteration EMD relative to Interpolation EMD are significant, especially for the high missing percentage. The real GNSS position time series of eight stations were selected to further evaluate the performance of Iteration EMD with an average missing percentage 8.15 %. Principal Component Analysis (PCA) was performed on the filled time series, which is used to assess the interpolation performance of Iteration EMD and Interpolation EMD. The results show that Iteration EMD can preserve variance 75.9 % with the first three Principal Components (PC), more than 66.5% of interpolation EMD. Therefore, we can conclude that Iteration EMD is an efficient interpolation method for GNSS position time series, which can make full use of available information in existing time series to fill the missing data.
Contractile dysfunction and fatal arrhythmias are the hallmarks of myocardial ischemia/reperfusion (I/R) injury. Pterostilbene has notable cardioprotective effects, but its main mechanisms are not fully understood. Here, we investigated the effect of PTE on myocardial hemodynamics, arrhythmias, inflammatory/oxidative responses, and the causal role of the JAK2/STAT3 pathway in rats with acute myocardial I/R injury. Sixty male 7-8 months Sprague-Dawley rats (n=10/each group) experienced in vivo model of myocardial I/R injury through 40-min LAD coronary artery occlusion and subsequent 24-h reperfusion. PTE at concentrations of 5 and 25 mg/kg was intraperitoneally administered to rats five min before reperfusion. Cardiac hemodynamics, reperfusion-induced ventricular arrhythmias, infarct size, inflammatory cytokines, oxidative stress markers, the activity of the JAK2/STAT3 pathway were measured as the endpoints. Administration of PTE to I/R-injured rats recovered myocardial contractile function and reduced infarct size and ventricular arrhythmias counts and incidence in a dosedependent manner. PTE at 25 mg/kg significantly and more potently reduced the levels of inflammatory mediators NF-κB, TNF-α, and IL-1β, suppressed intracellular ROS production, augmented the activity of glutathione, and manganesesuperoxide dismutase, and upregulated the JAK2 and STAT3 phosphorylation. Importantly, pretreatment of rats with Ag490 as a JAK2 inhibitor significantly abolished the cardioprotective and signaling effects of PTE in I/R rats. PTE exerts significant protective effects on reducing arrhythmias and myocardial infarction and enhancing cardiac function by stimulating JAK2/STAT3-related suppression of inflammatory and oxidative reactions in the I/R injury setting.
Cieľom štúdie bolo preskúmať koreláty jazykovej emocionality a verbálneho vyjadrenia emócií u bilingválnych osôb pomocou analýzy prierezu výskumných štúdií. Materinský jazyk (L1) sa vyznačuje väčšou jazykovou emocionalitou v porovnaní s ďalším bilingvistom ovládaným jazykom (L2). Emocionálna sila jazyka koreluje s vekom osoby v čase jeho osvojenia, emocionálnymi skúsenosťami pri jeho osvojení a špecifickým kultúrnym kontextom. Jazyk s väčšou emocionálnou silou sa používa na verbálne vyjadrenie emocionálnych zážitkov najmä v emocionálne nabitých situáciách a v prípade pozitívnej valencie prežívaných emócií. V prípade negatívnej valencie sa preferuje menej emocionálny jazyk, ktorý ponúka odstup od emocionálneho prežívania. Bilingvista v terapeutickom vzťahu môže spontánne či účelne prepínať medzi ovládanými jazykmi s cieľom regulovať intenzitu emocionálneho prežívania. Hoci terapia bilingválneho klienta predstavuje výzvu i pre profesionála, očakáva sa rešpektovanie zmien jazykového prejavu na strane klienta a cielená práca s jazykovým kontextom na strane terapeuta., The aim of this study is to examine the correlates of language emotionality and the verbal expression of emotions in bilingual persons by analysing a selection of research studies. In comparison with another language spoken by a bilingual, the mother tongue is characterised by a greater emotional charge. The emotional power of a language correlates with the age of the person and their emotional experience at the time of its learning as well as the specific cultural context. The language with a greater emotional charge is used to verbally express emotional experiences, specifically in emotionally charged situations and in the case of positive valence of experienced emotions. On the other hand, in the case of negative valence, the less emotional language that offers distance from emotional experience is preferred. In a therapeutic relationship, a bilingual can spontaneously or purposefully switch between languages in order to regulate the intensity of emotional experience. Although therapy with a bilingual client can be challenging for the professional, too, respect for changes in language expression on the part of the client and targeted work with the language context on the part of the therapist are expected., Kinga Izsóf Jurásová, Linda Kissová., and Obsahuje bibliografické odkazy