Monitoring changes in populations is fundamental for effective management. The West European hedgehog (Erinaceus europeaus) is of conservation concern in the UK because of recent substantial declines. Surveying hedgehogs is, however, problematic because of their nocturnal, cryptic behaviour. We compared the effectiveness of three methods (infra-red thermal camera, specialist search dog, spotlight) for detecting hedgehogs in three different habitats. Significantly more hedgehogs were detected, and at greater distance, using the camera and dog than the spotlight in amenity grassland and pasture; no hedgehogs were detected in woodland. Increasing ground cover reduced detection distances, with most detections (59.6%) associated with bare soil or mown grass; the dog was the only method that detected hedgehogs in vegetation taller than the target species' height. The additional value of surveying with a detection dog is most likely to be realised in areas where badgers (Meles meles), an intra-guild predator, are and/or where sufficient ground cover is present; both would allow hedgehogs to forage further from refuge habitats such as hedgerows. Further consideration of the effectiveness of detection dogs for finding hedgehogs in nests, as well as developing techniques for monitoring this species in woodland, is warranted.
Growing worldwide efforts to replace (reduce) animal testing and to improve alternative in vitro tests which may be more efficient in terms of both time, cost and scientific validity include also genotoxicity/mutagenicity endpoints. The aim of the review article was to summarize currently available in vitro testing approaches in this field, their regulatory acceptance and recommended combinations for classification of chemicals. A study using the combination of Comet Assay performed on two cell lines and the Chromosomal Aberration test on human peripheral lymphocytes was performed with the aim to predict the genotoxic potential of selected paraben esters, serving as a model chemical group. Parabens are widely used in consumer products as preservatives and have been reported to exhibit inconclusive results in numerous genotoxicity studies. The Comet Assay identified Ethylparaben and Benzylparaben as potentially genotoxic. The Chromosomal Aberration test revealed weak genotoxic potential in case of Ethylparaben and positive genotoxicity in case of Butylparaben, Propylparaben and Isopropylparaben. The main reasons for variability seem to be limited water solubility of parabens, determining their bioavailability at the cellular level, and absence of metabolic activation in the Comet Assay. The results confirmed that the Comet Assay should serve as a screening test and should not be used as a stand-alone method for classification of genotoxicity. The weight of evidence approach in risk assessment should be supported with data generated with the use of human relevant in vitro methods based on cells / tissues of human origin.
Pancreatic microcirculatory dysfunction emerged as a novel mechanism in the development of hypertension. However, the changes of pancreatic microcirculation profiles in hypertension remain unknown. Pancreatic microcirculatory blood distribution pattern and microvascular vasomotion of spontaneously hypertensive rats (SHRs) and Wistar Kyoto rats (WKYs) were determined by laser Doppler. Wavelet transform analysis was performed to convert micro-hemodynamic signals into timefrequency domains, based on which amplitude spectral scalograms were constructed. The amplitudes of characteristic oscillators were compared between SHRs and WKYs. The expression of eNOS was determined by immunohistochemistry, and plasma nitrite/nitrate levels were measured by Griess reaction. Additionally, endothelin-1, malondialdehyde, superoxide dismutase and interleukin-6 were determined by enzyme-linked immunosorbent assay. SHRs exhibited a lower scale blood distribution pattern with decreased average blood perfusion, frequency and amplitude. Wavelet transform spectral analysis revealed significantly reduced amplitudes of endothelial oscillators. Besides reduced expression of eNOS, the blood microcirculatory chemistry complements micro-hemodynamic profiles as demonstrated by an increase in plasma nitrite/nitrate, endothelin-1, malondialdehyde, interleukin-6 and a decrease of superoxide dismutase in SHRs. Here, we described abnormal pancreatic microcirculation profiles in SHRs, including disarranged blood distribution pattern, impaired microvascular vasomotion and reduced amplitudes of endothelial oscillators.
The complete mitochondrial genome of a pyraloid species, Palpita hypohomalia, was sequenced and analyzed. This mitochondrial genome is circular, 15,280 bp long, and includes 37 typical metazoan mitochondrial genes (13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes) and an A + T-rich region. Nucleotide composition is highly biased toward A + T nucleotides (81.6%). All 13 protein-coding genes (PCGs) initiate with the canonical start codon ATN, except for cox1 which is CGA. The typical stop codon TAA occurs in most PCGs, while nad2 and cox2 show TAG and an incomplete termination codon T, respectively. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) which lacks the dihydrouridine (DHU) arm. Comparative mitochondrial genome analysis showed that the motif "ATGATAA" between atp8 and atp6, and the motif "ATACTAA" between trnS2 and nad1 were commonly present in lepidopteran mitogenomes. Furthermore, the "ATAG" and subsequent poly-T structure, and the A-rich 3' end were conserved in the A + T-rich regions of lepidopteran mitogenomes. Phylogenetic analyses based on our dataset of 37 mitochondrial genes yielded identical topology for the Pyraloidea, and is generally identical with that recovered by a previous study based on multiple nuclear genes. In a previous study of the Crambidae, the Evergestinae was synonymized with Glaphyriinae; the present study is the first to clarify their close relationship with mitogenome data.
Sand-water slurry was investigated on an experimental pipe loop of inner diameter D = 100 mm with the horizontal, inclined, and vertical smooth pipe sections. A narrow particle size distribution silica sand of mean diameter 0.87 mm was used. The experimental investigation focused on the effects of pipe inclination, overall slurry concentration, and mean velocity on concentration distribution and deposition limit velocity. The measured concentration profiles showed different degrees of stratification for the positive and negative pipe inclinations. The degree of stratification depended on the pipe inclination and on overall slurry concentration and velocity. The ascending flow was less stratified than the corresponding descending flow, the difference increasing from horizontal flow up to an inclination angle of about +30°. The deposition limit velocity was sensitive to the pipe inclination, reaching higher values in the ascending than in the horizontal pipe. The maximum deposition limit value was reached for an inclination angle of about +25°, and the limit remained practically constant in value, about 1.25 times higher than that in the horizontal pipe. Conversely, in the descending pipe, the deposition limit decreased significantly with the negative slopes and tended to be zero for an inclination angle of about −30°, where no stationary bed was observed.
A new genus, Coniophloeus gen. n., is described for five species of South African weevils known from the Western and Eastern Cape and assigned to the tribe Embrithini Marshall, 1942: Coniophloeus squalidus (Boheman, 1842) comb. n. (type species, transferred from the genus Trachyphloeus Germar, 1817 and tribe Trachyphloeini Lacordaire, 1863), C. alternans sp. n., C. oberprieleri sp. n., C. robustus sp. n. and C. obrieni sp. n. All five species are illustrated and keyed.
As part of a modern review of all Australian Collembola families, a key is provided to the nine genera of Brachystomellidae currently known from Australia, their morphology is compared, their distribution within and outside Australia is noted and the high diversity of genera in southern regions emphasised. Three Australian genera are endemic, five are also found in South America, South Africa and/or New Zealand and one has a cosmopolitan distribution. The distribution, ecology and habitat preferences of Australian genera are compared. Two genera, Cassagnella Najt & Massoud and Rapoportella Ellis & Bellinger, are newly diagnosed and additions to the description of C. anomala Womersley are given. Australian Cassagnella species appear restricted to southern, humid regions and C. anomala possesses some characters that indicate it is adapted to living in habitats that are periodically flooded. A new species, Rapoportella edwardi sp. n. is described in the rarer genus from drier eucalypt forests. The effect of agricultural practices on an introduced species of Brachystomella is noted and its indicator value emphasised. Possible threats to the endemic genera and species are noted., Penelope Greenslade., and Obsahuje bibliografii
It is generally accepted that angiotensin II plays an important role in high blood pressure (BP) development in both 2-kidney-1- clip (2K1C) Goldblatt hypertension and in partial nephrectomy (NX) model of chronic kidney disease (CKD). The contribution of sympathetic nervous system and nitric oxide to BP control in these models is less clear. Partial nephrectomy or stenosis of the renal artery was performed in adult (10-week-old) male hypertensive heterozygous Ren-2 transgenic rats (TGR) and normotensive control Hannover Sprague Dawley (HanSD) rats and in Wistar rats. One and four weeks after the surgery, basal blood pressure (BP) and acute BP responses to the consecutive blockade of renin-angiotensin (RAS), sympathetic nervous (SNS), and nitric oxide (NO) systems were determined in conscious rats. Both surgical procedures increased plasma urea, a marker of renal damage; the effect being more pronounced following partial nephrectomy in hypertensive TGR than in normotensive HanSD rats with a substantially smaller effect in Wistar rats after renal artery stenosis. We demonstrated that the reninangiotensin system does not play so fundamental role in blood pressure maintenance during hypertension development in either CKD model. By contrast, a more important role is exerted by the sympathetic nervous system, the activity of which is increased in hypertensive TGR-NX in the developmental phase of hypertension, while in HanSD-NX or Wistar-2K1C it is postponed to the established phase. The contribution of the vasoconstrictor systems (RAS and SNS) was increased following hypertension induction. The role of NO-dependent vasodilation was unchanged in 5/6 NX HanSD and in 2K1C Wistar rats, while it gradually decreased in 5/6 NX TGR rats.
The event runoff coefficient (Rc) and the recession coefficient (tc) are of theoretical importance for understanding catchment response and of practical importance in hydrological design. We analyse 57 event periods in the period 2013 to 2015 in the 66 ha Austrian Hydrological Open Air Laboratory (HOAL), where the seven subcatchments are stratified by runoff generation types into wetlands, tile drainage and natural drainage. Three machine learning algorithms (Random forest (RF), Gradient Boost Decision Tree (GBDT) and Support vector machine (SVM)) are used to estimate Rc and tc from 22 event based explanatory variables representing precipitation, soil moisture, groundwater level and season. The model performance of the SVM algorithm in estimating Rc and tc is generally higher than that of the other two methods, measured by the coefficient of determination R2, and the performance for Rc is higher than that for tc. The relative importance of the explanatory variables for the predictions, assessed by a heatmap, suggests that Rc of the tile drainage systems is more strongly controlled by the weather conditions than by the catchment state, while the opposite is true for natural drainage systems. Overall, model performance strongly depends on the runoff generation type.
Growth and development rates in many insects are affected by photoperiod, which enables insects to synchronize their life histories with seasonal events, but this aspect of insect photoperiodism remains understudied. Here we use several experimental combinations of constant day length and temperature to determine whether there are quantitative developmental responses to photoperiod in the bug Scantius aegyptius and leaf beetle Timarcha tenebricosa. The thermal ecology of these two species is strikingly different: the former is thermophilic and active throughout summer and the latter is spring-active and avoids the hottest time of the year. In accordance with their contrasting natural thermal environments, S. aegyptius survives better and achieves a larger final body mass at the high experimental temperatures, while T. tenebricosa survives better and is heavier at the low experimental temperatures. Despite this polarity, long-day conditions accelerate larval development relative to a short-day photoperiod in both species, and this developmental response is stronger at low temperatures. Our re-visitation of previous literature in light of the new findings indicates that this similarity in photoperiodic response is superficial and that relatively faster development in midsummer is likely to have a different ecological role in summer- and spring-active species. In the former, it may allow completion of an additional generation during the favourable season, whereas in the latter, this acceleration likely ensures that the larval stage, which is vulnerable to heat, is completed before the onset of hot weather., Dmitry Kutcherov, Elena B. Lopatina, Sergei Balashov., and Obsahuje bibliografii