CARM1 i nteracts with numerous transcription factors to mediate cellular processes, especially gene expression. This is important for the maintenance of ESC pluripotency or intervention to tumorigenesis. Here, we studied epigenomic effects of two potential CARM1 m odulators: an activator (EML159) and an inhibitor (ellagic acid dihydrate, EA). We examined nuclear morphology in human and mouse embryonic stem cells (hESCs, mESCs), as well as in iPS cells. The CARM1 modulators did not function similarly in all cell type s. EA decreased the levels of the pluripotency markers, OCT4 and NANOG, particularly in iPSCs, whereas the levels of these proteins increased after EML159 treatment. EML159 treatment of mouse ESCs led to decreased levels of OCT4 and NANOG, which was accomp anied by an increased level of Endo -A. The same trend was observed for NANOG and Endo -A in hESCs affected by EML159. Interestingly, EA mainly changed epigenetic features of nucleoli because a high level of arginine asymmetric di- methylation in the nucleoli of hESCs was reduced after EA treatment. ChIP -PCR of ribosomal genes confirmed significantly reduced levels of H3R17me2a, in both the promoter region of ribosomal genes and rDNA encoding 28S rRNA, after EA addition. Moreover, EA treatment changed the nuclear pattern of AgNORs (silver -stained nucleolus organizer regions) in all cell types studied. In EA -treated ESCs, AgNOR pattern was similar to the pattern of AgNORs after inhibition of RNA pol I by actinomycin D. Together, inhibitory effect of EA on arginine methylation and effect on related morphological parameters was especially observed in compartment of nucleoli., M. Franek, S. Legartová, J. Suchánková, C. Milite, S. Castellano, G. Sbardella, S. Kozubek, E. Bártová., and Obsahuje bibliografii
The possible protective action of L-carnitine on neuronal excitability was studied in 21-day-old male Wistar rats with implanted electrodes. Administration of L-carnitine did not change the elicitation and duration of the epileptic seizures (cortical afterdischarges, ADs) in rats under normobaric oxygen atmosphere conditions. However, in animals exposed to 30 min hypobaric hypoxia the duration of the ADs was shortened after the second, fourth and sixth stimulation (in comparison with the first evoked ADs) while carnitine-treated rats retained their neuronal excitability and the duration of ADs was shortened only after the third stimulation., D. Marešová, H. Rauchová, K. Jandová, I. Valkounová, J. Koudelová, S. Trojan., and Obsahuje bibliografii
Let R be an associative ring with identity and F a class of R-modules. In this article: we first give a detailed treatment of Cartan-Eilenberg F complexes and extend the basic properties of the class F to the class CE(F). Secondly, we study and give some equivalent characterizations of Cartan-Eilenberg projective, injective and flat complexes which are similar to projective, injective and flat modules, respectively. As applications, we characterize some classical rings in terms of these complexes, including coherent, Noetherian, von Neumann regular rings, QF rings, semisimple rings, hereditary rings and perfect rings., Xiaorui Zhai, Chunxia Zhang., and Obsahuje seznam literatury
A new coccidian parasite of the genus Caryospora is described from a long nosed vine snake, Ahaetulla nasuta, Lacépède, 1789 from Thailand. Oocysts of Caryospora ahaetullae sp. n. are spherical or slightly subspherical, 24.8 (22.5-26.0) pm in diameter, with a single-layered wall about 0.75 pm thick. A micropyle or oocyst residuum is absent. One, rarely two, polar granules are present, 1.5-2.0 pm. Sporocysts are ellipsoidal, 18.5 x 14.2 (18.0-19.5 x 13.0-15.0) pm with prominent Stieda and substieda bodies. Sporocyst residuum present, consisting of numerous very small granules scattered among 8 sporozoites. Sporo-zoites, with prominent transverse striations anteriorly, are vermiform, 15.0 x 3.8 (13.5-16.5 x 3.5—4.5) pm (in situ), lying lengthwise and parallel within sporocyst. Each sporozoite contains a spherical anterior and spherical to subspherical posterior refractile bodies.
A new coccidian parasite of the genus Caryospora Léger is described from the colubrid snake Boiga dendrophila Boie, 1827 imported from Kalimantan. Oocysts of Caryospora kalimaniunensis sp. n. are spherical, 18.7 (17.0-20.0) pm in diameter, with a bilayered slightly pitted and brownish oocyst wall. A micropyle and oocyst residuum are absent. One globular polar granule is present in 45% of sporulated oocysts. Sporocysts are ovoidal to ellipsoidal, 14.5 (13.0-15.5) x 10.5 (10.0-11.0) pm, with prominent Stieda and substieda bodies. Sporocyst residuum is present as small granules scattered among sporozoites. Sporozoites are elongate, lying lengthwise and parallel within the sporocyst and completely filling the sporocyst. Each sporo-zoite contains a spherical anterior and posterior refractile bodies.
A new coccidian parasite of the genus Caryospora Léger, 1904 is described from the colubrid snake Psammophis schokari (Forskâl, 1775). Oocysts of Caryospora maxima sp. n. are spherical or subspherical, 43.0 (40.0-46.0) X 42.1 (40.0-44.0) pm, with smooth and bilayered oocyst wall ca. 2.0 μιη thick. A micropyle, oocyst residuum and polar granule are absent. Sporocysls are broadly ellipsoidal, 21.3 (21.0-22.0) x 16.3 (16.0-17.0) μιη, with smooth, colourless and unilayered sporocyst wall. Sticda and substieda bodies are present. Sporocyst residuum is present as compact mass of granules lying centrally between sporozoites. Sporozoitcs arc elongate, arranged head to tail within sporocyst. Caryospora maxima represents the first coccidian species described from Psammophis schokari.
Publikace vydaná v r. 2013 je určena zejména praktikům působícím v oblasti komunitní péče (terénní a ambulantní) o uživatele návykových látek. Kolektiv autorů popisuje praxi case managementu, způsobu práce, který se v mnoha "západních" zemích stal základním nástrojem komunitní péče o osoby v obtížných situacích, včetně osob závislých. I v českém prostředí se tento přístup postupně usazuje a dá se, [autoři Pavel Nepustil, Markéta Szotáková a kol. ; editor Lucie Grolmusová], and Obsahuje bibliografii