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6562. Isoforms of troponin in normal and diseased myocardium
- Creator:
- Michaela Adamcová and Václav Pelouch
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, troponiny, myokard, srdeční selhání, ontogeneze, troponins, myocardium, heart failure, ontogeny, isoforms of troponin, congenital heart disease, 14, and 612
- Language:
- English
- Description:
- M. Adamcová, V. Pelouch. and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6563. Isolation and characterisation of the first microsatellite markers for the European stag beetle, Lucanus cervus (Coleoptera: Lucanidae)
- Creator:
- McKeown, Niall J, Harvey, Deborah J, Healey, Amy J. E, Skujina, Ilze, Cox, Karen, Gange, Alan C, and Shaw, Paul W
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- brouci, roháčovití, genetika, biodiverzita, ekologie, saproxylické organismy, beetles, Lucanidae, genetics, biodiversity, ecology, saproxylic organisms, Coleoptera, simple sequence repeats, conservation, 2, and 59
- Language:
- English
- Description:
- The European stag beetle, Lucanus cervus, is recognised as a flagship species for biodiversity conservation. Although the species is widely distributed across Europe declines have led to it being granted protected or endangered status in a number of countries and regarded as "near threatened" by the IUCN. The integration of genetic approaches into conservation efforts is urgently needed but has been impeded to date by the lack of appropriate genetic markers. To provide such a resource the development of the first microsatellite loci for stag beetle is described. Loci were identified using two methods (i) enriched library cloning (ELC) and (ii) Restriction enzyme Associated DNA sequencing (RAD-Seq). Inefficient microsatellite detection using the ELC method suggests that RAD-Seq, or other Next Generation Sequencing based methods, may ultimately be more cost effective for obtaining informative suites of markers for this and other coleopteran species. 18 loci were characterised by genotyping 42 UK specimens collected as prey leftover/roadkill. All loci produced unambiguous genotypes and were polymorphic. Though preliminary, estimates of genetic variability suggest UK populations may be genetically depauperate. The microsatellite loci represent a suite of genetic markers that can be applied to non-invasive population monitoring and numerous other areas of Lucanus conservation and evolutionary research., Niall J. McKeown, Deborah J. Harvey, Amy J. E. Healey, Ilze Skujina, Karen Cox, Alan C. Gange, Paul W. Shaw., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6564. Isolation and characterization of 15 microsatellite markers for the highly invasive box tree moth Cydalima perspectalis (Lepidoptera: Crambidae)
- Creator:
- Bras, Audrey, Sauné, Laure, Roques, Alain, Rousselet, Jérôme, and Auger-Rozenberg, Marie-Anne
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- motýli, butterflies, Lepidoptera, Crambidae, Cydalima perspectalis, microsatellites, genetic diversity, invasive insect, 2, and 59
- Language:
- English
- Description:
- In this study, we report the development of a set of 15 polymorphic microsatellite markers for the box tree moth, Cydalima perspectalis (Walker), a highly invasive insect in Europe causing significant damage to natural and ornamental Buxus trees. The markers were characterized for four distant populations in both its native (China, two populations) and invasive ranges (Czech Republic and Turkey, one population each). The number of alleles ranged from 2 to 12. No marker significantly deviated from the Hardy-Weinberg equilibrium for all the populations sampled. These microsatellite markers are promising tools for further studies on the invasive pathways and dispersal pattern of the box tree moth in Europe., Audrey Bras, Laure Sauné, Alain Roques, Jérôme Rousselet, Marie-Anne Auger-Rozenberg., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6565. Isolation and characterization of cytosolic malate dehydrogenase from Trichomonas vaginalis
- Creator:
- Drmota, Tomáš, Tachezy, Jan, and Kulda, Jaroslav
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- Trichomonas vaginalis, trichomonads, anaerobic glycolysis, malate dehydrogenase, purification, and oxaloacetate
- Language:
- English
- Description:
- Malate dehydrogenase (EC 1.1,1,37.) (MDH) was purified to apparent homogeneity from the cytosolic fraction of the protozoan Trichomonas vaginalis Donné. The four step purification included ion-exchange chromatography (DEAE-Sephacel and Q-Sepharose, elution with NaCl) and affinity chromatography (Reactive Red Agarose, elution with NADH and NaCl). The enzyme was purified about 132-fold (30.6% yield) to a specific activity of 352 units mg~'. The Km values determined at pH 7.8 (pH optimum from 7.5 to 8.3) for oxaloacetate and NADH were 16.2 pM and 10.6 μΜ, respectively. The MDH activity was inhibited by the substrate, decreasing to 50% at about 1 mM concentration of oxaloacetate. The reverse reaction from malate to oxaloacetate showed a pH optimum around pH 9.5. The Km for malate and NAD* (determined at pH 7.8) were 1220 pM and 69.9 pM, respectively. SDS-PAGE analysis of the purified MDH revealed a single band with an apparent size of 34.5 kDa. The native molecular weight was estimated by HPLC gel filtration to be 60 kDa, which indicates that the T. vaginalis MDH exists as a dimer.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6566. Isolation and characterization of novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus (Coleoptera: Nitidulidae)
- Creator:
- Kleven, Oddmund and Endrestøl, Anders
- Format:
- print, počítač, and online zdroj
- Type:
- article, články, journal articles, model:article, and TEXT
- Subject:
- Zoologie, brouci, lesknáčkovití, beetles, Nitidulidae, Coleoptera, Thymogethes norvegicus, microsatellite loci, genetic diversity, 2, and 59
- Language:
- English
- Description:
- Here we describe 16 novel microsatellite loci in the genome of the dragonhead sap beetle Thymogethes norvegicus, which were identified using a next-generation sequencing approach. In 40 dragonhead sap beetles from a population in southern Norway, we found 2-4 alleles per locus. Expected heterozygosity ranged from 0.18-0.65. Three of the loci showed significant deviation from Hardy-Weinberg equilibrium. After correcting for multiple tests, one pairwise locus combination displayed significant linkage disequilibrium. These novel microsatellite loci will be useful for future population structure, genetic diversity and conservation genetic studies of the dragonhead sap beetle., Oddmund Kleven, Anders Endrestøl., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6567. Isolation of rat lung mast cells for purposes of one-week cultivation using novel percoll variant percoll PLUS
- Creator:
- Kubrycht, J., Hana Maxová, Otakar Nyč, and Luděk Vajner
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, hypoxie, hypoxia, lung mast cells, percoll, c-kit, progenitor, secretion, 14, and 612
- Language:
- English
- Description:
- Prolonged cultivation of separated rat lung mast cells (LMC) in vitro is necessary to better investigate a possible role of LMC in different stages of tissue remodeling induced by hypoxia. Rat lung mast cells (LMC) were sepa rated using a protocol including an improved proteolytic extracti on and two subsequent density gradient separations on Ficoll-P aque PLUS and a new generation of Percoll, i.e. Percoll PLUS. Instead of usual isotonic stock Percoll solution, an alternative “asymptotically isotonic” stock solution was more successful in our density separation of LMC on Percoll PLUS. Separated cells were cultivated for six days in media including stem cell factor, interleu kins IL-3 and IL-6, and one of two alternative mixtures of antibi otics. These cultivations were performed without any contaminatio n and with only rare changes in cell size and morphology. Model co-cultivation of two allogenic fractions of LMC often caused considerable rapid changes in cell morphology and size. In contrast to these observations no or rare morphological changes were found after cultivation under hypoxic conditions. In conclusions, we modified separation on Percoll PLUS to be widely used, altered LMC separation with respect to purposes of long-lasti ng cultivation and observed some model morphological changes of LMC., J. Kubrycht ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6568. Isolation of the cyanobacterial YFP-tagged photosystem I using GFP-Trap®
- Creator:
- Strašková, A., Knoppová, J., and Komenda, Josef
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- Cyanophyta, assembly factor, pigment-protein complex, two-dimensional electrophoresis, 2, and 581
- Language:
- Multiple languages
- Description:
- A strain of Synechocystis sp. PCC 6803 expressing the yellow fluorescent protein (YFP) fused to the C-terminus of the PsaF subunit of PSI has been constructed and used to isolate native PSI complexes employing the GFP-Trap®, an efficient immunoprecipitation system which recognizes the green fluorescent protein (GFP) and its variants. The protein analysis and spectroscopic characterization of the preparation revealed an isolate of trimeric and monomeric PSI complexes, which showed minimal unspecific contamination as demonstrated by comparison with the wild type control. Interestingly, we detected CP43 subunits of PSII and small amounts of PSII core complexes specifically pulled-down with the YFP-PSI, supporting the association of PSII assembly modules and intermediate assembly complexes with PSI, as observed in our previous studies. The results demonstrate that the GFP-Trap® system represents an excellent tool for studies of PSI biogenesis and interconnection of PSI and PSII assembly processes., A. Strašková, J. Knoppová, J. Komenda., and Obsahuje bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
6569. Isometric composition operators on weighted Dirichlet space
- Creator:
- Han, Shi-An and Zhou, Ze-Hua
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- matematika, Dirichletova úloha, mathematics, Dirichlet problem, composition operator, weighted Dirichlet space, isometry, 13, and 51
- Language:
- English
- Description:
- We investigate isometric composition operators on the weighted Dirichlet space {D_\alpha } with standard weights {(1 - {\left| z \right|^2})^\alpha },\alpha > - 1 . The main technique used comes from Martín and Vukotić who completely characterized the isometric composition operators on the classical Dirichlet space D. We solve some of these but not in general. We also investigate the situation when {D_\alpha } is equipped with another equivalent norm., Shi-An Han, Ze-Hua Zhou., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6570. Isomorphisms and several characterizations of Musielak-Orlicz-Hardy spaces associated with some Schrödinger operators
- Creator:
- Yang, Sibei
- Format:
- print, bez média, and svazek
- Type:
- model:article and TEXT
- Subject:
- mathematics, Musielak-Orlicz-Hardy space, Schrödinger operator, L-harmonic function, isomorphism of Hardy space, atom, molecule, 13, and 51
- Language:
- English
- Description:
- Let L := −Δ + V be a Schrödinger operator on\mathbb{R}^{n} with n\geqslant 3 and V\geqslant 0 satisfying \Delta ^{-1}V\in L^{\infty }(\mathbb{R}^{n}). Assume that φ: {R}^{n} × [0,∞) → [0,∞) is a function such that φ(x,,) is an Orlicz function, φ(•, t) \in A_{\infty }({R}^{n}) (the class of uniformly Muckenhoupt weights). Let w be an L-harmonic function on {R}^{n} with 0< C_{1}\leq w\leq C_{2}, where C_{1} and C_{2} are positive constants. In this article, the author proves that the mapping H_{\phi ,L} (\mathbb{R}^n ) \mathrel\backepsilon f \mapsto wf \in H_\phi (\mathbb{R}^n ) is an isomorphism from the Musielak-Orlicz-Hardy space associated with L,H_{\phi ,L} (\mathbb{R}^n ), to the Musielak-Orlicz-Hardy space H_\phi (\mathbb{R}^n ) under some assumptions on φ. As applications, the author further obtains the atomic and molecular characterizations of the space H_{\phi ,L} (\mathbb{R}^n ) associated with w, and proves that the operator {( - \Delta )^{ - 1/2}}{L^{1/2}} is an isomorphism of the spaces H_{\phi ,L} (\mathbb{R}^n ) and H_\phi (\mathbb{R}^n ). All these results are new even when φ(x, t) ≔ t^{p}, for all x \in \mathbb{R}^{n} and t \in [0,∞), with p ∞ (n/(n + μ_{0}), 1) and some μ_{0} \in (0, 1]., Sibei Yang., and Obsahuje seznam literatury
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public