Four fluorescence parameters [Fv/Fm = the intrinsic efficiency of energy conversion via photosystem 2 (PS2); Fv'/Fm'= the efficiency of energy conversion via PS2 in the light; P = fraction of absorbed radiant energy utilized for photosynthesis; and D = fraction of absorbed radiant energy dissipated as heat] were measured on leaves of seven species of succulents having epidermal windows. While the function of leaf windows has reportedly been to increase absorption of radiant energy and, hence, the rate of photosynthesis in these species, recent evidence indicates that this translucent portion of epidermal tissue, lacking chlorophyll, may also result in photoinhibition in these species, especially for those with growth habits aboveground. Species with aboveground and belowground growth habits were compared with their leaf windows covered with reflective tape and with windows unobstructed. Results showed no increase in photoinhibition for these species resulting from the radiant energy penetrating the window tissue. Although the efficiency of the photosynthetic mechanism was not significantly influenced by the additional radiant energy provided by the window for individual species, there were significant differences in the efficiencies of radiant energy capture (Fv'/Fm') and utilization (P) between the two growth habits. Species with an aboveground growth habit were less efficient in radiant energy utilization compared with the species having a belowground growth habit. and K. J. Egbert, C. E. Martin.
Low temperature (LT) is one of the major factors that limit crop production and reduce yield. To better understand the cold-tolerance mechanism in the plantains, a sensitive cultivar Williams (Musa acuminata AAA cv. Williams) and a tolerant cultivar Cachaco (Musa paradisiaca ABB cv. Dajiao) were used. LT resulted in increased malondialdehyde (MDA) content, elevated contents of hydrogen peroxide (H2O2) and superoxide radical (O2.-), and decreased photochemical efficiency (Fv/Fm) and net photosynthetic rate (PN), but cv. Cachaco showed better LT tolerance than cv. Williams. After LT treatment for 120 h, total scavenging capability (DPPH. scavenging capability) in Williams showed a significant decrease but no significant alternations was found in Cachaco. Ascorbate peroxidase (APX) and peroxidase (POD) displayed a significant increase but superoxide dismutase (SOD) showed no significant alternations and catalase (CAT) showed a significant decrease in Cachaco after 120 h of LT treatment. All the four antioxidant enzymes above showed a significant decrease in Williams after 120 h of LT treatment. Our results suggest that higher activities of APX, POD, SOD, and DPPH. scavenging capability to a certain extent can be used to explain the higher cold tolerance in the plantain, which would provide a theoretical guidance for bananas production and screening cold-resistant variety. and Q. Zhang ... [et al.].
The article deals with the influence of phase modulated synthetic jet on the aerodynamics of the hump in a closed test section of the Eiffel-type wind tunnel. Three experimental methods of measurement techniques of this phenomenon, were used: the pressure profile using the Kiel total pressure probe, the velocity profile using the CTA probe and the visualization of the flow field using the hot film and the thermo camera. The experimental results with and without the influence of the synthetic jet were compared, as well the impact of the phase shift of the neighbouring synthetic jets. As a reference case, the flow around the hump without the influence of the syntheic jet was selected. The results of the measurement are presented in figures and compared. and Obsahuje seznam literatury
The pathophysiological processes underlying the development of diabetic osteopenia has not hitherto been elucidated. Induction of streptozotocin diabetes leads in our experiments to decrease of bone density, ash, mineral content and to thinner cortical width compared to control male rats. In order to investigate the pathogenetic role of bone resorption by osteoclasts in streptozotocin-induced diabetes, we determined the circulating levels of tartrate-resistant acid phosphatase (TRAP), a biochemical marker for bone resorption. Plasma TRAP values in diabetic rats did not differ from their corresponding controls. Streptozotocin diabetes by itself did not have any effect on the weight of seminal vesicles which are highly testosterone-dependent. Low doses of nitric oxide cause bone resorption, but higher doses of NO inhibit bone resorbing activity. We examined the effect of L-NAME (inhibitor of nitric oxide production) after six weeks of administration to diabetic rats. There was no further significant loss of bone mineral density, ash and mineral content or tibia weight in diabetic rats treated with L-NAME. L-NAME itself did not decrease bone metabolism. In our study no evidence of an increased bone resorption was found. Our results have indicated that a predominance of bone resorption over bone formation is not involved in the pathogenesis of diabetes-associated osteopenia. Inhibition of NO neither increased osteoclastic activity (TRAP) nor induced osteopenia in L-NAME-treated rats. This suggests a possibility that NO is not involved in the pathogenesis of diabetic osteopenia., P. D. Broulík, M. Haluzík, J. Škrha., and Obsahuje bibliografii
Substantial evidence shows that the frequency of hydrological extremes has been changing and is likely to continue to change in the near future. Non-stationary models for flood frequency analyses are one method of accounting for these changes in estimating design values. The objective of the present study is to compare four models in terms of goodness of fit, their uncertainties, the parameter estimation methods and the implications for estimating flood quantiles. Stationary and non-stationary models using the GEV distribution were considered, with parameters dependent on time and on annual precipitation. Furthermore, in order to study the influence of the parameter estimation approach on the results, the maximum likelihood (MLE) and Bayesian Monte Carlo Markov chain (MCMC) methods were compared. The methods were tested for two gauging stations in Slovenia that exhibit significantly increasing trends in annual maximum (AM) discharge series. The comparison of the models suggests that the stationary model tends to underestimate flood quantiles relative to the non-stationary models in recent years. The model with annual precipitation as a covariate exhibits the best goodness-of-fit performance. For a 10% increase in annual precipitation, the 10-year flood increases by 8%. Use of the model for design purposes requires scenarios of future annual precipitation. It is argued that these may be obtained more reliably than scenarios of extreme event precipitation which makes the proposed model more practically useful than alternative models.
Longwave radiation, as part of the radiation balance, is one of the factors needed to estimate potential evapotranspiration (PET). Since the longwave radiation balance is rarely measured, many computational methods have been designed. In this study, we report on the difference between the observed longwave radiation balance and modelling results obtained using the two main procedures outlined in FAO24 (relying on the measured sunshine duration) and FAO56 (based on the measured solar radiation) manuals. The performance of these equations was evaluated in the April–October period over eight years at the Liz experimental catchment and grass surface in the Bohemian Forest (Czech Republic). The coefficients of both methods, which describe the influence of cloudiness factor and atmospheric emissivity of the air, were calibrated. The Penman-Monteith method was used to calculate the PET. The use of default coefficient values gave errors of 40–100 mm (FAO56) and 0–20 mm (FAO24) for the seasonal PET estimates (the PET was usually overestimated). Parameter calibration decreased the FAO56 error to less than 20 mm per season (FAO24 remained unaffected by the calibration). The FAO56 approach with calibrated coefficients proved to be more suitable for estimation of the longwave radiation balance.
The decrease in inorganic phosphate (Pi) content of 10-d-old Phaseolus vulgaris L. plants did not affect rates of photosynthesis (PN) and respiration (RD), leaf growth, and adenylate concentration. Two weeks of phosphate starvation influenced the ATP content and leaf growth more than PN and RD. The ATP concentration in the leaves of 15- and 18-d-old phosphate deficient (-P) plants after a light or dark period was at least half of that in phosphate sufficient (+P, control) plants. Similar differences were found in fresh and dry matter of leaves. However, PN declined to 50 % of control in 18-d-old plants only. Though the RD of -P plants (determined as both CO2 evolution and O2 uptake) did not change, an increased resistance of respiration to KCN and higher inhibition by SHAM (salicylhydroxamic acid) suggested a higher engagement of alternative pathway in respiration and a lower ATP production. The lower demand for ATP connected with inhibition of leaf growth may influence the ATP producing processes and ATP concentration. Thus, the ATP concentration in the leaves depends stronger on Pi content than on PN and RD. and M. Mikulska, J.-L. Bomsel, A. M. Rychter.
Photosynthetic induction responses to a sudden increase in photosynthetic photon flux density (PPFD) from lower background PPFD (0, 25, 50, and 100 μmol m-2 s-1) to 1 000 μmol m-2 s-1 were measured in leaves of Fagus crenata, Acer rufinerve Siebold & Zucc., and Viburnum furcatum growing in a gap and understory of a F. crenata forest in the Naeba mountains. In the gap, A. rufinerve exhibited more than 1.2-fold higher maximum net photosynthetic rate (PNmax) than F. crenata and V. furcatum. Meanwhile, in the understory F. crenata exhibited the highest PNmax among the three species. The photosynthetic induction period required to reach PNmax was 3-41 min. The photosynthetic responses to increase in PPFD depended on the background PPFD before increase in PPFD. The induction period required to reach PNmax was 2.5-6.5-fold longer when PPFD increased from darkness than when PPFD increased from 100 μmol m-2 s-1. The induction period was correlated with initial PN and stomatal conductance (gs) relative to maximum values before increase in PPFD. The relationship was similar between the gap and the understory. As the background PPFD increased, the initial PN and gs increased, indicating that the degrees of biochemical and stomata limitations to dynamic photosynthetic performance decreased. Therefore, photosynthetic induction responses to increase in PPFD became faster with the increasing background PPFD. The differences in time required to reach induction between species, as well as between gap and understory, were mainly due to the varying of relative initial induction states in PN and gs at the same background PPFD. and M. Naramoto, Q. Han, Y. Kakubari.
Toxoplasma gondii, a parasite infecting 20-60% of humans in various countries, influences the behaviour of infected animal and human hosts. Infected human subjects have changes in several of Cattell's and Cloninger's personality factors. Recently, three independent studies have shown that Rh-positive subjects are protected against the T. gondii-induced changes of reaction times and increased risk of traffic accidents. Here we searched for evidence of similar effects of RhD phenotype on toxoplasmosis- or aging-associated changes in the personality profile of about 302 blood donors. We found that Rh-positive and Rh-negative subjects responded differently to toxoplasmosis. In addition to the already known effects of toxoplasmosis on novelty seeking, self transcendence, superego strength and protension, we also found effects of RhD phenotype on ego strength, protension, and praxernia, as well as opposite effects of toxoplasmosis on ego strength, praxernia, ergic tension and cooperativeness in Rh-positive and Rh-negative subjects. Moreover, our results indicate that RhD phenotype might influence not only the effect of toxoplasmosis but also the effect of aging on specific personality traits.
The use of environmentally-friendly materials in hydraulic engineering (e.g. the stone lining of weirs at levees) calls for the more accurate estimation of the discharge coefficient for broad-crested weirs with a rough crest surface. However, in the available literature sources the discharge coefficient of broad-crested weirs is usually expressed for a smooth crest. The authors of this paper have summarized the theoretical knowledge related to the effect of weir crest surface roughness on the discharge coefficient. The method of determination of the head-discharge relation for broad-crested weirs with a rough crest surface is proposed based on known discharge coefficient values for smooth surfaces and on the roughness parameters of the weir. For selected scenarios the theoretical results were compared with experimental research carried out at the Laboratory of Water Management Research, Faculty of Civil Engineering (FCE), Brno University of Technology (BUT). and Používání přírodě blízkých materiálů ve vodním hospodářství (např. kamenná opevnění povrchu přelivů v ochranných hrázích) vyvolává požadavek přesnějšího stanovení součinitele průtoku pro přelivy se širokou a hydraulicky drsnou korunou. V dostupných literárních pramenech je součinitel průtoku přelivů se širokou korunou obvykle vyjádřen pouze pro hladký povrch koruny přelivu. Autoři článku shrnuli teoretické poznatky týkající se vlivu drsnosti povrchu koruny přelivu na součinitel průtoku a navrhli metodu pro stanovení způsobu hydraulického výpočtu konzumční křivky přepadu přes přeliv se širokou a drsnou korunou. Postup vychází ze znalosti součinitele průtoku přelivu s hladkou korunou a charakteristik drsnosti. Pro vybrané scénáře byly teoreticky vypočítané hodnoty porovnány s výsledky experimentálního výzkumu uskutečněného v Laboratoři vodohospodářského výzkumu Ústavu vodních staveb, Fakulty stavební, Vysokého učení technického v Brně.