DNA barcoding surveys of small insects usually extract DNA from either a complete insect or a leg. Little is known about how to optimize DNA quantity and quality from different insect parts while preserving a morphological voucher. Here, we quantify DNA yield from different body parts (antenna, hind leg, forewing, hind wing and abdomen) of the micro-moth Cameraria ohridella (Lepidoptera: Gracillariidae) using fluorescent nucleic acid stain (PicoGreen). Samples were preserved in 100% ethanol or dried for three weeks. Our experiment was designed to encompass practical sampling options during fieldwork. DNA quality was assessed by PCR amplification of the mitochondrial COI barcode fragment. In addition, we compared PCR amplification using Platinum® Taq and Qiagen DNA Polymerase and quantified sequence success of amplified DNA. We show that overall, dry parts showed higher eluted DNA yields. PCR and sequencing success rate were slightly higher for dry tissue than ethanol-preserved parts. We also show that Platinum® Taq yielded the highest PCR success rate and that all dry tissues are sequenceable. The optimal strategy for DNA barcoding surveys is therefore to mount micro-Lepidoptera specimens in the field for morphological analysis and sample tissues (hind legs are favoured) from dried samples at a later time (several weeks) in the lab for DNA barcoding using preferentially Platinum® Taq. If larger amounts of DNA are required (i.e. for nuclear gene sequencing), several legs from one side of the specimen or the abdomen should be preserved in pure ethanol., Carlos Lopez-Vaamonde ... [et al.]., and Obsahuje seznam literatury
In this report, we dealt with ventricular activation abnormalities in 30 patients with previous non-Q myocardial infarction (Mi) by means of the CARD1AG 128.1 device, which enables analysis of ECGs, VCGs and body surface potential maps. The diagnosis was verified by left ventriculography, echocardiography and perfusion scintigraphy. Twenty-nine healthy subjects served as the control group. Morphological findings confirmed the presence of a significant subgroup with serious left ventricular asynergy. Seven electrocardiological variables, which significantly differed from control values, disclosed that non-Q Ml is responsible for localized activation time prolongation, and that inferoposterior scars tend to delay the entire activation of ventricles, and to cause disturbances of the terminal depolarization phase together with a decrease in voltage production during QRS. Lesions of the anterior wall and the apicomesial part of the inferoposterior wall affect the distribution of the Q wave more often than the posterior basal ones. The probability of such abnormalities increases with the degree of asynergy. Some VCG criteria increase the sensitivity of electrocardiological analysis. These parameters will be used for evaluating the diagnostic value of electrocardiological analysis in the chronic non-Q Ml. Non-Q myocardial infarctions represent a heterogeneous group of infarctions from both electrophysiological and morphological aspects.
Persistent Scatterer Interferometry (PSI), a remote sensing technique, is used for detecting surface deformation in the cities of Prague and Ostrava. PSI is able to detect vertical movements with an accuracy of less than 1 mm for a long time series of the SAR data, but the maximum detectable rate of movement is only a few centimetres per year. This technique is quite suitable for detecting recent movements in most Prague localities. On the other hand, in Ostrava and its surroundings, affected by undermining, where subsidences (1992-2006) amount to decimetres per year, movements cannot be fully detected by the PSInSAR technique. The paper presents results of analysing PSI data for two localities in Prague and one locality in the Ostrava areas. The localities are strictly situated in built-up areas with many suitable reflectors. Data from the ERS-1/2 and ENVISAT satellites covering a 13-year period for the Prague (1992-2005) and a 14-year period for the Ostrava (1992-2006) area were used. Annual movement velocities and time-series of reflectors were determined. At these three localities, where different types of movements were identified, the application and possible limitations of PSI in urban areas are shown., Pavel Kadlečík, Vladimír Schenk, Zuzana Seidlová and Zdeňka Schenková., and Obsahuje bibliografii
The main objective of this paper is to explain how the application of various interpolation methods influence the determination of vertical crustal movements at any given point. The paper compares several methods of interpolation and verifies their suitability, including kriging, minimum curvature, nearest neighbor, natural neighbor, polynomial regression, inverse distance to a power, and triangulation with linear interpolation. The calculations show that the chosen interpolation method has significant influence on the final result of the study. Nearest neighbor method was chosen to be the best., Kamil Kowalczyk, Jacek Rapinski and Marek Mroz., and Obsahuje bibliografii
We introduce an one-dimensional thermodynamical particle model which is efficient in predictions about a microscopical structure of animal/human groups. For such a model we present analytical calculations leading to formulae for time clearance distribution as well as for time spectral rigidity. Furthermore, the results obtained are reformulated in terms of vehicular traffic theory and consecutively compared to experimental traffic data.
An improved theoretical formulation is proposed here to predict the moment field induced in laterally loaded RC rectangular stiffened plates including the effect of strength and stiffness of the internal stiffening beams. Design charts are also presented for their quick proportioning. The study reveals the effect of strength and stiffness of the internal beams on the moment field induced in the plate-system which was not considered, currently, by various methods prescribed by different design codes. The use of stiffened-plates becomes mandatory in buildings to accomodate some architectural constraints as well as for satisfying the serviceability criterion of design codes. This type of a structural system is efficient, economical and readily constructible in most of common materials. Moreover, it can be built as a monolithic unit or as a composite system comprising a plate cast in concrete and beams constructed in prestressed concrete, fabricated sections in steel, and so forth. A working example is presented to demonstrate the validity and efficiency of the simplified approach in comparison to finite element based design and other code prescribed methods. and Obsahuje seznam literatury a Appendix A
Je předložena první část plánovaného souboru malých prací, určených pro tento časopis. Práce se týká věcí, podle našeho názoru důležitých pro analytickou kvalitu v klinické laboratoři. Celý soubor prací se bude zabývat tématy metrologie (charakteru předmětů měření), statistikou, vnitřní kontrolou kvality, externím hodnocením kvality a kritickými postřehy v uvedených oblastech. Příspěvky jsou napsány s maximální snahou po jednoduchosti podle konceptu ASAP-„as simple as possible“. V prvním příspěvku byl aplikován tento přístup pro odvození požadavků na analytickou kvalitu z hodnot biologických variabilit: je diskutována situace při měření koncentrace Na v krevním séru. Analytická kvalita tohoto měření je akceptovatelná, pokud dlouhodobě sledované výsledky vykazují stabilitu v rozmezí 1 %. Této stability je možné dosáhnout., This is the first article in a series of small, „loose“ contributions to this journal. The articles touch, what we think, is important about analytical quality in the medical laboratory The articles will address topics such as metrology (philosophy of the measurand), statistics, internal quality control (IQC), external quality assessment (EQA), critiques to the discipline. All contributions follow the ASAP-concept, meaning As Simple As Possible. Here, we explore the „ASAP“ concept for analytical quality specifications derived from biological variation. The application of the concept results for the great majority of the mainstream serum-, plasma-, and blood-analytes in CVa values within boundaries of 0.4 – 15 % and for Delta-SE within boundaries of 0.2 – 10 %. The take-home message is: analytical quality in the medical laboratory must NOT be viewed in absolute terms („a CVa of 2 % is good“), BUT in relative terms. A CVa of 2 % is good for S-cholesterol analysis, but NOT for S-Na analysis!, Stepman H., Stöckl D., and Literatura 11
V základním vyjádření je statistická síla pravděpodobnost dosažení statistické významnosti. Tři faktory (effect-size – velikost zamýšleného efektu), α, n), spolu se statistickou silou, tvoří uzavřený systém – pokud jsou všechny tři ustaveny, je tím zároveň i kompletně určen čtvrtý. Cílem silové analýzy je najít vhodnou rovnováhu mezi těmito faktory při zohlednění podstatných cílů studie. Ilustrujeme význam role velikosti zamýšleného efektu, α, a n na síle jednostranného F-testu, abychom poskytli celkový obrázek o pojetí konceptu statistické síly (testu)., In very basic terms, statistical power is the likelihood of achieving statistical significance. Three factors (effect-size, α, n), together with power, form a closed system – once any three are established, the fourth is completely determined. The goal of a power analysis is to find an appropriate balance among these factors by taking into account the substantive goals of a study. We exemplify the role of effect-size, α, and n on the power of a 1-sided F-test and give a general illustration of the power concept., Stepman H., Stöckl D., and Literatura 8