Kinetics of non-photochemical reduction of the photosynthetic intersystem electron transport chain by exogenous NADPH was examined in osmotically lysed spinach chloroplasts by chlorophyll (Chl) fluorescence measurements under anaerobic condition. Upon the addition of NADPH, the apparent F0 increased sigmoidally, and the value of the maximal slope was calculated to give the reduction rate of plastoquinone (PQ) pool. Application of 5 µM antimycin A lowered significantly both the ceiling and the rate of the NADPH-induced Chl fluorescence increase, while the suppressive effect of 10 µM rotenone was slighter. This indicated that dark reduction of the PQ pool by NADPH in spinach chloroplasts under O2-limitation condition could be attributed mainly to the pathway catalysed sequentially by ferredoxin-NADP+ oxidoreductase (FNR) and ferredoxin-plastoquinone reductase (FQR), rather than that mediated by NAD(P)H dehydro-genase (NDH). and Ming-Xian Jin, Hualing Mi.
The paper reconstructs the fate of a codex from the library of Matthias Corvinus, King of Hungary and Bohemia, which is in the possession of the National Library in Prague now. From the middle of the 16th century, it was deposited at Uhrovec Castle in Slovakia, which belonged to the Zay family; at the end of the 17th century, it was moved to Bohemia – first to the library of the de Suys family at Kounice Castle, later to the castle of the Thuns in Choltice, from where it was donated to the Capuchin monastery in Prague-Hradčany in the 1720s. and Obsahuje obrazovou přílohu