Adverse effect of caffeine consumption has been well documented in animals and in human beings. However, here we studied the influence of caffeine exposure on seedling growth of Arabidopsis and tobacco plants. Retardation in the seedling growth of these plants was observed when grown on MS medium plates containing 1 mM caffeine and their growth retarded further upon increasing the concentration of caffeine to 5 mM. Retardation in seedling size including both root and shoot size, yellowing and decrease in chlorophyll content of seedlings upon caffeine treatment indicated that caffeine exposure induced early senescence in plants. Therefore, the influence of caffeine exposure on transcript expression and activity of Rubisco in tobacco and Arabidopsis seedlings was monitored. Caffeine exposure has been found to decrease the expression and activity of Rubisco in both the plants. Hence, this study documents that caffeine exposure retarded seedling growth and one reason for this could be its negative effect on Rubisco. and P. Mohanpuria, S. K. Yadav.
Partitioning of 14C-labelled photosynthates to various parts of un-pruned tea clones TV1 and TV25 was assessed in vivo by exposing maintenance leaves to 14CO2 at monthly intervals throughout the year. The plants from shoot apex to root tip were divided into twelve components to assess the allocation and retention of 14C-photosynthates by the maintenance foliage. Out of the total photosynthates produced by the maintenance leaves, only 11.08 % was allocated to the commercially useful harvestable two and a bud shoots which is accepted as the harvest index of tea. The photosynthetically active maintenance leaves retained 19.05 % while 24.56 % was distributed to the branches. The bottom and the top parts of the trunk utilized 7.44 and 7.21 %, respectively. The thick roots at the base of the trunk, medium sized roots, pencil size roots, and feeder roots imported 7.28, 7.72, 7.65, and 8.01 % of 14C assimilates, respectively. Except retention by leaves, all the plant parts of vigorous clone TV25 required higher percentage of assimilates than TV1. The mean quantities of net photosynthates utilized by the stem and the roots were 69.37 and 30.63 %, respectively. and T. S. Barman, J. K. Saikia.
A decrease of F,„ followed with a ceitain delay by an increase of was detected in the cells of Synechococcus elongatus in the first 120 min of the photoinhibitory treatment at 56 <>0 (growth temperature). Then F,n started to rise in parallel with F,, and this process proceeded widi the same rate both in the light and in the dark at 56 °C after light treatment. On tíie contrary, an increase of F^ observed during the light treatment at 15 °C was largely reversed after subsequent transfer of the ceUs to the dark at 56 <>€ but F,q remained nearly unchanged during the dark incubation.
During the growth cycle of micropropagated Juglans regia plantlets, phosphoenolpyruvate carboxylase (PEPC) activity was aiways higher than initial ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activity. The ratio of PEPC/RuBPCO activities was higher during the first part of the exponential phase of growth and lower during the second part. The variations of PEPC activity were parallel to the soluble protein content and the variations of initial and total RuBPCO activities were parallel to the chlorophyll accumulation.
Two cultivars of bean, Tacarigua and VUL 73-401 were subjected to two levels of water stress followed by rewatering. During the water deficit period, water potential (Ψ№), osmotic potential (Ψ5) and relative water content (rwc) decreased with an associated decrease of ribulose-l,5-bisphosphate carboxylase activity (RuBPC), protein and chlorophyll (Chi) contents. In cv. Tacarigua, the % decrease of RuBPC was less marked than that of protein content. There was a significant correlation among the components of leaf water status and the measured photosynthetic parameters. Groups of water stressed plants were rewatered, when reached -1.25 to -1.60 MPa. During the recovering period all components of leaf water status increased and reached the control values on the 6th day after rewatering. Chi content increased above the control values. Protein content and RuBPC activity also increased during the rewatering period, but the % increases in protein content and RuBPCase activity were not parallel.
Wheat provides a unique genetic system in which variable sink size is available across the ploidies. We characterized monocarpic senescence in diploid, tetraploid, and hexaploid wheat species in flag leaf from anthesis up to full grain maturity at regular intervals. Triticum tauschii Acc. cv. EC-331751 showed the fastest rate of senescence among the species studied and the rate of loss per day was highest in terms of photosynthesis rate, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) content, and flag leaf N content coupled with a higher rate of gain in grain N content. Cultivars Kundan and HD 4530 maintained high flag leaf N content throughout grain filling as compared to the diploids and showed a slower rate of senescence. RuBPCO content was higher in the diploids as compared to Kundan and HD 4530 at anthesis. However, the rate of decline in RuBPCO content per day was also higher in the diploids. This degradation in RuBPCO was mediated by high endoproteolytic activities in the diploids which in turn supported its higher rate of N mobilization as compared to the tetraploid and hexaploid wheat. Acidic endopeptidases were responsible for the mobilization of flag leaf nitrogen in wheat across ploidy levels (r=-0.582, p<0.01). and B. Srivalli, R. Khanna-Chopra.
Intact chloroplasts were isolated from dark-senescing primary barley (Hordeum vulgare L.) leaves in order to study selective ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) degradation by the stromal and membrane fractions. RuBPCO specific degradation was estimated and characterised applying sensitive avidin-biotin ELISA method with non-modified or oxidatively modified biotinylated RuBPCO (BR) as substrates. Distinct proteolytic activities were detected. They differed in ATP and divalent metal ion dependence, protease inhibitory profile, and dynamics in the time-course of dark-induced senescence. The results supported involvement of ATP- and metal ion-dependent serine type proteolytic activity against non-modified BR early in induced senescence and appearance of ATP-independent activity at later stage. Active oxygen-modified BR was degraded by ATP-independent serine-type protease probably containing essential SH-groups and requiring divalent metal ions. and L. Simova-Stoilova, K. Demirevska-Kepova, Z. Stoyanova.