The illuminated manuscripts in Zittau are only known to some extent. The two-volume Vesperale and Matutinale (A I, A VI) from the second decade of the fifteenth century was created for the Karlov Augustinian Monastery in Prague. The painted decoration is the work of the Master of the Hasenburg Missal, who represents the highest stage of fine style. The Missal of the Prague Diocese (A VII) is from the early fifteenth century. The decoration is the work of two illuminators, led by the Master of the Roudnice Psalter, although the share of the second illuminator - the Master of Paul's Gospel – is more extensive. Hitherto unknown is the Antiphonary (A IV) from the second decade of the fifteenth century. The small share of the primary illuminator is based on the Master of the Antwerp Bible. The Zittau Gradual (A III) is dated 1512; its primary illuminator was Janíček Zmilelý of Písek. The Gradual (A V) was created in 1435 for the parish church in Zittau by commander of the Commenda of Johannites Johann Gottfried von Goldbergu. The decoration was evidently created in Vratislav by the Master of the Bible of Banken. The Vesperal and Matutinal (A II) from the end of the fifteenth century was perhaps designated for the Commenda of Johannites in Zittau. The decoration is Saxon work.
The illuminated manuscripts in Zittau are only known to some extent. The two-volume Vesperale and Matutinale (A I, A VI) from the second decade of the fifteenth century was created for the Karlov Augustinian Monastery in Prague. The painted decoration is the work of the Master of the Hasenburg Missal, who represents the highest stage of fine style. The Missal of the Prague Diocese (A VII) is from the early fifteenth century. The decoration is the work of two illuminators, led by the Master of the Roudnice Psalter, although the share of the second illuminator - the Master of Paul's Gospel – is more extensive. Hitherto unknown is the Antiphonary (A IV) from the second decade of the fifteenth century. The small share of the primary illuminator is based on the Master of the Antwerp Bible. The Zittau Gradual (A III) is dated 1512; its primary illuminator was Janíček Zmilelý of Písek. The Gradual (A V) was created in 1435 for the parish church in Zittau by commander of the Commenda of Johannites Johann Gottfried von Goldbergu. The decoration was evidently created in Vratislav by the Master of the Bible of Banken. The Vesperal and Matutinal (A II) from the end of the fifteenth century was perhaps designated for the Commenda of Johannites in Zittau. The decoration is Saxon work.
The Municipal Library in Bautzen houses a lot of Czech manuscripts which are dealt with by special literature, but the illuminations of which are known very scarcely. The decoration of the collection of work by Jan Hus (Ms. fol. 51) can be narrowed to the middle of the 15th century; the present date of 1412 is based on an entry in fol. 137v and is untenable because it is the text, not the decoration which came into existence in this year. The depictions – the figural and the heraldic ones – were added to the manuscripts supplementally; it is probably a picture of Jan Hus mounting the pulpit and a coat of arms of a member of the Hroznata family, maybe of the Kladruby abbot Bušek of Vrtba or of the front warrior of the Catholic Side, Burian of Gutštejn. The collection of theological-juridical texts (Ms. fol. 56) contains moreover yearly records; only one text item (Řeči besední) can be attributed to Tomáš Štítný of Štítné. The decoration of the codex is limited only to one fi gural illumination and one ornamental initial. Its style is quite advanced; it can most likely be dated to the 1470s.
The Municipal Library in Bautzen houses a lot of Czech manuscripts which are dealt with by special literature, but the illuminations of which are known very scarcely. The decoration of the collection of work by Jan Hus (Ms. fol. 51) can be narrowed to the middle of the 15th century; the present date of 1412 is based on an entry in fol. 137v and is untenable because it is the text, not the decoration which came into existence in this year. The depictions – the figural and the heraldic ones – were added to the manuscripts supplementally; it is probably a picture of Jan Hus mounting the pulpit and a coat of arms of a member of the Hroznata family, maybe of the Kladruby abbot Bušek of Vrtba or of the front warrior of the Catholic Side, Burian of Gutštejn. The collection of theological-juridical texts (Ms. fol. 56) contains moreover yearly records; only one text item (Řeči besední) can be attributed to Tomáš Štítný of Štítné. The decoration of the codex is limited only to one fi gural illumination and one ornamental initial. Its style is quite advanced; it can most likely be dated to the 1470s.
The Municipal Library in Bautzen houses a lot of Czech manuscripts which are dealt with by special literature, but the illuminations of which are known very scarcely. The decoration of the collection of work by Jan Hus (Ms. fol. 51) can be narrowed to the middle of the 15th century; the present date of 1412 is based on an entry in fol. 137v and is untenable because it is the text, not the decoration which came into existence in this year. The depictions – the figural and the heraldic ones – were added to the manuscripts supplementally; it is probably a picture of Jan Hus mounting the pulpit and a coat of arms of a member of the Hroznata family, maybe of the Kladruby abbot Bušek of Vrtba or of the front warrior of the Catholic Side, Burian of Gutštejn. The collection of theological-juridical texts (Ms. fol. 56) contains moreover yearly records; only one text item (Řeči besední) can be attributed to Tomáš Štítný of Štítné. The decoration of the codex is limited only to one fi gural illumination and one ornamental initial. Its style is quite advanced; it can most likely be dated to the 1470s.
The study of leaf vascular systems is important in order to understand the fluid dynamics of water movement in leaves. Recent studies have shown how these systems can be involved in the performance of photosynthesis, which is linked to the density of the vascular network per unit of leaf area. The aim of the present study was to highlight the correlation between a leaf vein density (VD) and net photosynthetic rate (PN), which was undertaken using a digital camera, a stereoscopic microscope, and a light source. The proposed hypothesis was tested, for the first time, on the leaves of two cultivars of Vitis vinifera (L.). A significant difference was found between the VD of mature leaves of the two cultivars. VD was also significantly correlated with the maximum leaf PN. These findings support the hypothesis that the vascular system of grape leaves can be correlated with leaf photosynthesis performance., M. Pagano, P. Corona, P. Storchi., and Obsahuje bibliografii
An overview is given on the fluorescence imaging of plants. Emphasis is laid upon multispectral fluorescence imaging in the maxima of the fluorescence emission bands of leaves, i.e., in the blue (440 nm), green (520 nm), red (690 nm), and far-red (740 nm) spectral regions. Details on the origin of these four fluorescence bands are presented including emitting substances and emitting sites within a leaf tissue. Blue-green fluorescence derives from ferulic acids covalently bound to cell walls, and the red and far-red fluorescence comes from chlorophyll (Chl) a in the chloroplasts of green mesophyll cells. The fluorescence intensities are influenced (1) by changes in the concentration of the emitting substances, (2) by the internal optics of leaves determining the penetration of excitation radiation and partial re-absorption of the emitted fluorescence, and (3) by the energy distribution between photosynthesis, heat production, and emission of Chl fluorescence. The set-up of the Karlsruhe multispectral fluorescence imaging system (FIS) is described from excitation with UV-pulses to the detection with an intensified CCD-camera. The possibilities of image processing (e.g., formation of fluorescence ratio images) are presented, and the ways of extraction of physiological and stress information from the ratio images are outlined. Examples for the interpretation of fluorescence images are given by demonstrating the information available for the detection of different developmental stages of plant material, of strain and stress of plants, and of herbicide treatment. This novel technique can be applied for near-distance screening or remote sensing. and C. Buschmann, G. Langsdorf, H. K. Lichtenthaler.
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBPCO) and RuBPCO binding protein (BP) were isolated from barley leaves. RuBPCO was dissociated into subunits under denaturing conditions. Polyclonal antibodies against RuBPCO, RuBPCOBP and RuBPCO large subunit (LS) were raised. Inununoblotting analyses showed that anti-RuBPCO antibodies did not cross-react with BP. Anti-BP antibodies cross-reacted with RuBPCO smáli subunit (SS) and reacted but more slightly with RuBPCO LS.
The distribution of the carboxylating enzyme nbulose-l,5-bisphosphate carboxylase/oxygenase (RuBPCO) and an enzyme of the photorespiratory pathway (glycine decarboxylase) was determined within the leaf tissue by immunocytochemical techniques in C3, C4 and C3:C4 intermediate species. The specificity of the method for all the materiál was demonstrated by sodium dodecyl sulphate acrylamide gels and Western blotting of crude protein extracts. In the C3 species (wheat) the enzymes were located in chloroplasts (RuBPCO) and mitochondria (glycine decarboxylase) of mesophyll cells, while in the three "classical" C4 standards, i.e. Zea mays ("NADP-ME" type). Panicům maximum ("PCK" type) and P. turgidum ("NAD-ME" type), these were found exclusively in the respective organelles of the bundle sheath. In the intermediate species, RuBPCO was not compartmented as it was located in chloroplasts of mesophyll and bundle sheath cells. Yet glycine decarboxylase was found exclusively in bundle sheath mitochondria. InAristida funiculata, a C4 species with "non-classical" leaf structure, RuBPCO was found in chloroplasts of both the inner and outer bundle sheaths and glycine decarboxylase was located exclusively in mitochondria of the inner bundle sheath cells. It is suggested that A. funiculata may be a C4 species with C3:C4-like intermediate characteristics based on the observed distribution of glycine decarboxylase, although gas-exchange characteristics of this species are required before any reclassification can be considered.
Using an air-conditioned single-plant chamber, shoots of two dwarf beán cultivars were exposed for 5 oř 6 h to O3 concentrations, ranging from 0.3 to 0.8 pmol mol**. Diuing exposure water vapour, CO2, and O3 exchange rates of shoots were monitored with the aim to quantify possible differences between cultivars in sensitivity of stomata and mesophyll to O3 uptake. Aíter exposure changes in the water holding capacity of the treated leaves were also estůnated, combining pressure bomb, 3-gauge, and leaf chamber techniques. Rates of change of stomatal (Vg) and mesophyll (Vg,) conductances increased linearily with increase in the absorbed O3 flux via the stomata (Qg), the direshold for stomatal response being lower than that for mesophyll response. Above the threshold values of Qg, sensitivity of V„ to Qg was lower than ďiat of Vg. The water holding capacity of leaves decreased with increase in Qg, although no distinct threshold Qg was found. Cultivar differences in O3 sensitivities of Vg were statístically distinguishable on the stomatal segment of CO2 uptake route ordy.