The oligomeric state of photosystem 2 (PS2) complex in soybean leaves treated with saturating irradiance was studied by non-denaturing polyacrylamide gel electrophoresis (PAGE) and gel filtration chromatography. PS2 dimers resolved by non-denaturing PAGE accounted for about 75 % of total PS2 complex and there was no significant difference in the ratio of PS2 dimer to monomer between samples from saturating irradiance-treated and fully dark-adapted leaves. Furthermore, BBY particles were resolved into four chlorophyll-enriched fractions by gel filtration chromatography. From their molecular masses and protein components, these fractions were deduced to be PS2 dimer, PS2 monomer, oligomeric light-harvesting complex 2 (LHC2), and monomeric LHC2. Also, no change in the proportion of PS2 dimer in total PS2 was observed in the granal region of thylakoid membranes from soybean leaves after saturating irradiation. Hence the dimer is the predominant natural form of PS2 in vivo and no monomerisation of PS2 dimer occurs during saturating irradiance-induced photoinhibition in soybean leaves. and Shi-Qing Cai ... [et al.].
Count von Manderscheid-Blankenheim´s library at Blankenheim Castle was closed after Napoleon´s army invaded the Rhineland in 1794. The last owner of the castle, Augusta and Filip Krisitan von Sterrnberg-Manderscheid took some of the books to Prague where they became parts of the Sternberg Library, which itself was incorporated into Prince Lobkowicz´s library, with which it was transferred in 1928 to the Czech National Library collection. This study provides information on the discovery of the uniquely preserved catalogue of the Blankenheim Castle library, compiled sometime between 1789 and 1794. It has been used to successfully identify a total of 29 manuscripts in the National Library collection. The study concludes with an edition of the Blankenheim manuscript catalogue and brief descriptions of the discovered codices.