This paper focuses on the development of various types of drawn linear ornamentation and the changes in their functions in the manuscripts of late antiquity, Byzantium (to the 15th century) and the Latin West (up to and including the 11th century). This subject is examined in a comparison of the above cultural spheres and within the context of decoration in period applied art (particularly gold and textile work) and monumental painting and sculpture associated with architecture. The basics are also outlined for a study of the linear decoration of gothic manuscripts, which will be covered in another paper.
Characterisation of proteases degrading ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO, EC: 4.1.1.39) was studied in the cowpea leaf during monocarpic senescence 3 and 9 d after flowering (DAF), representing early and mid pod fill. The stage at 3 DAF coincided with decrease in the metabolic parameters characterising senescence, i.e., contents of total soluble proteins, RuBPCO, and leaf nitrogen. At 9 DAF, there was a decline in total soluble proteins and an appearance of a 48 kDa cysteine protease. Characterisation of the proteases was done using specific inhibitors. Subcellular localisation at 3 DAF was studied by following the degradation of RuBPCO large subunit (LSU) in the vacuole lysates using immunoblot analyses. Cysteine proteases played a predominant role in the degradation of RuBPCO LSU at the crude extract level. At 9 DAF, expression of cysteine protease isoforms was monitored using polyclonal antibodies against papain and two polypeptides of molecular masses 48 and 35 kDa were observed in the vacuole lysates. We confirmed thus the predominance of cysteine proteases in the vacuoles during different stages of pod development in cowpea leaf. and B. Srivalli, Sudhakar Bharti, Renu Khanna-Chopra.