This article refers to the rich vegetation on the rocky slopes along the Achensee (Tyrol, Northern calcareous Alps, Karwendelalpen) described by Professor Anton Kerner 150 years ago. The vegetation today is much less diverse, the subalpine species abundant in the last centuries are absent while the species of mixed mountainous forest prevail. The abundance of the Heather (Calluna vulgaris), which was not mentioned by A. Kerner, may indicate acidification due to acid rain. And the retreat of subalpine species may indicate the on going change to a warmer climate. and Jarmila Kubíková.
Incubation of maize NADP-malic enzyme with tetranitromethane (TNM) resulted in a total loss of enzyme activity. The loss of enzyme activity was not observed at pH 6.3 but at pH 8.0. NADP-malic enzyme was inactivated to almost 90 % by incubation with an 80-fold molar excess of TNM for 5 min at 30 °C. The substrate malate or Mg2+ alone gave no protection, while NADP provided considerable protection. NADP in the presence of malate and Mg2+ totally protected the enzyme activity, suggesting that tyrosine residue may be located at or near the active site of maize NADP-malic enzyme. The spectral analysis of the modified enzyme indicated that modification of at least one tyrosine residue per subunit resulted in complete loss of the enzyme activity. The fluorescence study of unmodified and modified enzymes postulated that essential tyrosine residue at maize NADP-malic enzyme is possibly involved in malate binding. and S. R. Rao, B. G. Kamath, A. S. Bhagwat.