To explore the debated phylogenetic relationship of two Hesperiidae subfamilies, Pyrginae and Eudaminae, and contribute to the understanding of the evolution of mitogenomic architecture in butterflies, we sequenced the complete mitogenome of Tagiades vajuna. The mitogenome is a typical circular duplex molecule of 15,359 bp. Apart from the standard 22 tRNAs, it has a tandem duplication of trnS(AGN) and trnE, which is unique in lepidopteran insects. Comparison with Ctenoptilum vasava indicates that the trnS1 duplication is not an ancestral state shared with other species of Tagiadini. Independent origin of the trnS1 duplications was further confirmed by the reconstruction of the ancestral character state based on the topology of the phylogram. Furthermore, comparative analysis of mitogenomes with and without tRNA duplications indicates that tRNA duplication does not alter the codon usage pattern. The mitogenome has negative AT- and GC-skews, and it is highly A+T-biased (79.7%). The AT-rich (or control) region (283 bp) contains "ATAGA" and "ATTTA" motifs. Regarding the phylogenetic analysis, we found that removal of the third codon position (3CP) from datasets used for the mitochondrial phylogenomics of Hesperiidae is likely to produce results that are more consistent: Pyrginae were rendered paraphyletic by Eudaminae in both analyses of the dataset from which the 3CP was removed (13 PCGs + all RNAs), but inclusion of the 3CP resulted in a destabilized topology, resulting in both monophyly and polyphyly. We conclude that even shallow-phylogenies of insects should pay close attention to compositional and mutational biases in mitogenomes., Fang-Fang Liu, Yi-Ping Li, Ivan Jakovlić, Xiang-Qun Yuan., and Obsahuje bibliografii
Příspěvek nabízí jak nezbytné základní informace o tandemové výuce, tak osobní zkušenost autorky s realizací tandemové výuky fyziky na Gymnáziu Nový Jičín, která probíhala v prvním pololetí školního roku 2019/2020 u žáků 2. ročníku tohoto gymnázia. Cíle, které byly v rámci tandemové výuky fyziky sledovány, zahrnovaly prohloubení spolupráce učitele fyziky z gymnázia s odborníkem (v našem případě s vysokoškolským pedagogem z Katedry fyziky Přírodovědecké fakulty Ostravské univerzity), ověření, zda může tandemová výuka vést ke zlepšení kvality výuky fyziky, a ověření, zda může mít tandemová výuka pozitivní vliv na postoje žáků k fyzice. Po každé vyučovací hodině proběhla mezi vyučujícími reflexe s důrazem na zhodnocení použitých edukačních forem a metod a jejich vlivu na poznávací procesy žáků. Zjištění směřující k ověření stanovených cílů byla získána analýzou odpovědí z evaluačních dotazníků, jež vyplnili všichni žáci, kteří tandemovou výuku absolvovali. Průběžně byly s žáky vedeny také rozhovory. Součástí příspěvku jsou výsledky evaluace, upozornění na výhody i případná úskalí tandemové výuky fyziky, stejně jako osobní pohled a zkušenost autorky., The paper deals with tandem education and the author's personal experience with tandem physics teaching at Nový Jičín Grammar School ( first term of the 2019/2020, second class). The aims of tandem physics teaching include improving cooperation of a teacher with an expert (in this case it was a university lecturer, from the Department of Physics, the Faculty of Science, University of Ostrava). Verification of whether tandem education leads to improvement in physics education and has a positive impact on students' attitude to physics was also carried out. After each lesson, there was reflection among the teachers with an emphasis on the utilised educational forms and method and their influence on the cognitive processes of students. Verification of the goals were obtained by analysing answers in evaluation questionnaires, which were completed by all students who had finished the tandem education. Interviews were also conducted with students on an ongoing basis. This part of the paper includes the results of the evaluation, advantages and potential difficulties of tandem physics teaching, as well as the author's personal view and experience., Jana Škrabánková., and Obsahuje bibliografické odkazy
Fenomén tandemového kojení nebyl v psychologickém výzkumu zatím příliš zachycen. Tandemové kojení bývá praktikováno některými matkami, které následují trend tzv. kontaktního rodičovství. Při tandemovém kojení matka kojí mladší i starší dítě zároveň. Se čtyřmi ženami, které se hlásí ke kontaktnímu rodičovství a tandemově kojí dítě v batolecím věku spolu s jeho starším sourozencem v předškolním věku, byly provedeny hloubkové rozhovory. Pro analýzu dat byla použita interpretativní fenomenologická analýza (IPA), s jejíž pomocí byla identifikována čtyři hlavní témata: 1) Snaha udržet kojení za každou cenu – od útlumu kojení v těhotenství, po prudký nárůst po porodu až k ideálu samoodstavu; 2) Matka uprostřed – „Připadám si, že jsem pořád … pořád mezi dětma…fyzicky a psychicky… uprostřed“; 3) Tandemové kojení jako jistota pro starší dítě v novém uspořádání rodiny a snaha matky o podporu sourozeneckých vztahů; 4) Podpora i kritika tandemového kojení vedoucí k upevnění přesvědčení. and The phenomenon of tandem nursing has not been much captured by psychological research. Tandem nursing is practiced by some mothers who follow the trend of attachment parenting. In tandem nursing, the mother breastfeeds both the younger and the older child at the same time. In-depth interviews were conducted with four women who claimed to practice attachment parenting and breastfed their toddler child together with their older sibling at preschool age. Interpretative phenomenological analysis (IPA) was used to analyze the data, and four main themes were identified: 1) Efforts to maintain breastfeeding at all costs - from the decline of breastfeeding in pregnancy to the sharp increase after childbirth to the ideal of selfreinstatement; 2) Mother in the middle - "I feel like I'm still...always in the middle...physically and mentally...between the children"; 3) Tandem nursing as a safeguard for the older child in the new family structure and the mother's efforts to support sibling relationships; 4) Support and criticism of tandem nursing leading to reinforcement of beliefs.
„Round and round“ dance belongs to thegroup of rotative dances, i.e. dances that are characteristic by the rotation of the pair around the common axis. The accompanying melodies to the „round and round“ dance represent in the context of the Czech folk songs a special musical-dancing type. Many collectors disclosed the deep roots and importance of this type (among them, Božena Němcová, Ludvík Kuba, Otakar Zich, Čeněk Holas, Jindřich Jindřich). The point for departure for further analytical studies represented the most complete possible heuristics, in other words, the gathering of all accessible mentions and documents about the „round and round“ dance. The first study of the proposed three-part cycle brings about the descriptions of the dance in the collections from the 19th and the first halfof the 20th century, many authentic testimonies, glosses, comments and analyses that help to set up the concrete image of this ancient dance culture. They also offers the information about the latent appearance of this musical-dancing type in the collections of songs and dances of Southern and eastern Bohemia and engages in the concrete form of the dancing step and the position of the „round and round“ dance in the system of the Czech folk dances.
A survey of proteocephalidean cestodes found in the firewood catfish Sorubimichthys planiceps (Spix et Agassiz) from the Amazon River is provided. The following taxa parasitic in S. planiceps are redescribed on the basis of their type specimens and material collected recently in the Amazon River, near the type localities in Brazil, and in Iquitos, Peru: Monticellia lenha Woodland, 1933; Nomimoscolex lenha (Woodland, 1933) (syn. Proteocephalus lenha Woodland, 1933); and Monticellia megacephala Woodland, 1934, for which a new genus, Lenhataenia, is proposed, with L. megacephala (Woodland, 1934) comb. n. as its type and only species. The new genus is a member of the Monticelliinae, i.e. has all genital organs in the cortex, and is most similar to Chambriella in possessing biloculate suckers and lacking a metascolex. It differs in the morphology of the cirrus-sac that contains a strongly coiled, thick-walled internal sperm duct (vas deferens) and a muscular cirrus of the appearance typical of most proteocephalideans, whereas that of Chambriella is sigmoid, with voluminous, tightly sinuous thick-walled internal sperm duct. In addition, Lenhataenia possesses a well developed internal musculature, whereas the internal musculature of Chambriella is weakly developed, formed by a low number of muscle fibres. The scolex morphology and distribution of microtriches of Peltidocotyle lenha (Woodland, 1933) (syn. Othinoscolex lenha Woodland, 1933 and Othinoscolex myzofer Woodland, 1933), Chambriella sp. and Choanoscolex sp. are described using scanning electron microscopy. The two latter taxa may be new for science and are reported from S. planiceps for the first time .
The erythrinid fish Hoplias malabaricus in Paraguay harbour two species of proteocephalid cestodes, Proteocephalus regoi sp. n. and Nomimoscolex matogrossensis Rego et Pavanelli, 1990. The former species differs from most South American members of Proteocephalus from fishes by: 1) presence of a small apical organ, 2) distribution of vitellaria, which do not reach to posterior margin of segment and 3) posterior position of vagina. Nomimoscolex matogrossensis is redescribed on the basis of type and recent material, with emphasis given to the morphology of genital organs and scolex, which possesses an apical organ. Both species possess a dense network of osmoregulatory canals in the postacetabular region of both scolex and neck. Proteocephalus regoi sp. n. is the first representative of the subfamily Proteocephalinae parasitizing erythrinid fish.
Chronic volume overload (VO) on the left ventricle (LV) augments redox stress and activates matrix metalloproteinase (MMP) which causes the endocardial endothelial-myocyte (EM) disconnection leading to myocardial contractile dysfunction. VO-induced MMP-9 activation impairs cardiac functions, in part by endothelial endocardial apoptosis, but the role of MMP-9 on EM functions remains obscure. We conjecture that chronic VO activates MMP-9 and causes EM uncoupling. Arteriovenous fistula (AVF) was created in genetically identical wild type (WT) mice (FVB/NJ) and MMP-9 knockout mice (MMP-9KO, FVB.Cg-MMP9tm1Tvu/J). Sham-operated mice were used as controls. Before experimentation the phenotype analysis of MMP-9KO mice was carried out. In-gel-gelatin zymography for MMP-9 activation was performed on LV homogenates. The EM functions were determined on LV rings using tissue myobath. We report a decrease in MMP-9 activity in left ventricular myocardial extracts in MMP-9 deficient mice after AVF. The responses to drugs affecting cardiac functions (acetylcholine (Ach), nitroprusside and bradykinin) were attenuated in AVF mice suggesting the impairment of EM coupling. Interestingly, the EM functions were restored in the MMP-9 deficient mice after AVF. We suggest a direct cause-and-effect relationship between MMP-9 activation and EM uncoupling in LV myocardium after chronic VO and the possible involvement of MMP-9 in myocardial contractile performance., K. S. Moshal, W. E. Rodriguez, U. Sen, S. C. Tyagi., and Obsahuje bibliografii a bibliografické odkazy