High incidence of thrombosis and venous thromboembolism was reported in patients with COVID-19. In this study, we focused on analysis of thrombophilic mutations performed without a standard DNA extraction step. In one hundred of COVID-19 positive outpatients, real-time PCR for Leiden mutation in the FV gene and G20210A mutation in the FII gene was carried out from DNA extracts and modified whole blood samples, and their cycle threshold (Ct) values were evaluated. In the extracts, healthy homozygotes (wt/wt), heterozygotes (M/wt), and homozygous carriers of Leiden mutation (M/M) provided median Ct values of 18.5, 19.4/22.0, and 20.9. In the whole blood, Ct values were 25.3 (wt/wt), 24.8/27.2 (M/wt), and 26.9 (M/M). Median Ct values for G20210A in the extracts were 19.6 for homozygotes (wt/wt), and 19.7/20.4 for heterozygous carriers. The whole blood samples provided Ct values of 23.9 in healthy homozygotes and 26.3/27.2 in heterozygotes for G20210A mutation. No homozygous subjects for G20210A and no double heterozygotes (for Leiden and G20210A mutations) were found. Despite significant differences in the Ct values, genotyping showed complete result concordance of the DNA extracts and the whole blood samples. The integrity and amplificability of DNA molecules in the whole blood samples during 28 days of deep freezing, interrupted by four cycles of thawing, did not significantly change. In conclusion, we demonstrated a new protocol for the detection of the thrombophilic mutations via real-time PCR on the modified whole blood of COVID-19 positive patients. The blood modification was reliable, easy, cheap, and saving costs and turnaround time of the whole laboratory process.
The thermoluminescence signals from leaflets of the same pea plant varied gradually according to their developmental stage. The AG emission, due to a back flow of electrons towards photosystem 2 (PS2) along a cyclic/chlororespiratory pathway, was stronger in mature leaves than in the growing ones. These age-related variations could be explained by a higher capacity of cyclic electron flow in mature leaves.
In the following essay-manifesto, Contradictions editor Joseph Grim Feinberg lays out his view of the journal as a platform for confronting the central contradictions of post-communism, working through the problems of Central and Eastern Europe in global context, and seeking the continued contemporary relevance of the history of emancipatory and critical thought. Contradictions, he writes, should enable philosophy and its neighboring fields to engage with this region, at this moment, while telling world history something that no other time or place has told it before.
During the last decades several studies in cognitive psychology have shown that many of our actions do not depend on the reasons that we adduce afterwards, when we have to account for them. Our decisions seem to be often influenced by normatively or explanatorily irrelevant features of the environment of which we are not aware, and the reasons we offer for those decisions are a posteriori rationalisations. But exactly what reasons has the psychological research uncovered? In philosophy, a distinction has been commonly made between normative and motivating reasons: normative reasons make an action right, whereas motivating reasons explain our behaviour. Recently, Maria Alvarez has argued that, apart from normative (or justifying) reasons, we should further distinguish between motivating and explanatory reasons. We have, then, three kinds of reasons, and it is not clear which of them have been revealed as the real reasons for our actions by the psychological research. The answer we give to this question will have important implications both for the validity of our classifications of reasons and for our understanding of human action.
Purkinje fibers were the first discovered component of the cardiac conduction system. Origin ally described in sheep in 1839 as pale subendocardial cells, they were found to be present, although with different morphology, in all mammalian and avian hearts. Here we review differences in their appearance and extent in different species, summarize the current state of knowledge of their function, and provide an update on markers for these cells. Special emphasis is given to popular model species and human anatomy., D. Sedmera, R. G. Gourdie., and Obsahuje bibliografii a bibliografické odkazy
In this paper we present the first insight about the impact of the COVID epidemic on the pri-mary housing market in Poland, with a focus on Warsaw which is the largest market. We ex-plain the structural features that allowed the market to return to pre-shock levels after the pandemic shock. Contrary, after the 2007-2008 global financial crisis the negative consequences lasted for several years. This time a sharp monetary policy and fiscal intervention was carried out. Moreover, the developer sector is much more mature, has expanded its production capaci-ties. We show empirically that the monopolistic competition of developers allowed them to restrict excessive demand that was observed before the COVID broke out. In this way they were able to increase prices despite the economic problems. Another important structural change was the increased housing demand, mainly for investment housing, which was fi-nanced predominantly with cash and contributed to the development of the rental market. We approximate the investment demand, which was generated by private households that pur-chased flats for rental, with the help of a simple demand and supply model.
The coffee plant is native to shaded environments and its seedlings are often produced in shaded nurseries. However, some nursery managers, in an effort to improve the acclimation of seedlings to field conditions after transplantation, produce seedlings in full sun exposure. In this study, the morphological and physiological parameters of arabica coffee (Coffea arabica) seedlings produced in full sun (T1) and in shade (T2) were examined. The biomass accumulation and relative growth rate of T1 and T2 seedlings were similar. The T1 seedlings had less biomass allocation to shoots, a lower leaf mass ratio and a lower leaf area ratio; however, they had a greater net assimilation rate (rate of increase in plant mass per unit leaf area), which was associated with a greater net photosynthetic rate. There were no alterations in the concentrations of total chlorophylls or in the chlorophyll a/b ratio when comparing T1 and T2 seedlings. No indications of photoinhibition or photooxidative damage were observed in the T1 plants, which were shown to have a more robust antioxidant system than the T2 plants. Seedlings transferred from shade to full sun (T3) were not capable of utilising the incident extra light to fix CO2. These seedlings showed a remarkable nocturnal retention of zeaxanthin and a significantly increased deepoxidation state of the xanthophyll cycle, even at predawn, but the activity of antioxidant enzymes was lower than in the T1 and T2 plants. Despite the acclimation capacity of T3 seedlings to the new light environment, they exhibited chronic photoinhibition and considerable photooxidative damage throughout the seven days following the transfer to full sun exposure. We further discuss the practical implications of producing coffee seedlings in full sunlight and under shade. and G. A. B. K. Moraes ... [et al.].
In this paper, a theoretical approach of a wideband preamplifier using a high-end operation amplifier OPA847 will be presented. This device is designed to be used for a low noise, wide band measuring. The level of optimization is based on a noise model for the electrical part of the receiver. During the process of amplifier designing, all the noise components have been investigated separately, using operational amplifiers models. Although this preamplifier was designed to serve for the purposes of flame sensors evaluate, it can be also used to other purposes requiring wide band amplifier with low noise, under only a minimal modification. and V tomto příspěvku bych rád přiblížil teoretický postup návrhu
širokopásmového předzesilovače, využívajícího moderní integrovaný
předzesilovač OPA847, který je určen pro nízkošumová
měření. Stupeň optimalizace je založen na šumovém modelu pro
elektrickou část přijímače. Během návrhu zesilovače byla každá
šumová složka zkoumána zvlášť s využitím modelu operačního
zesilovače. Přestože byl tento předzesilovač navržen pro účely
měření detektorů plamene. Může být s výhodou využit všude jinde,
kde je vyžadován širokopásmový, nízkošumový předzesilovač a to
jen s minimálními úpravami.