The coccinellid Harmonia axyridis is a recent arrival in the UK and is an intraguild predator of the entomopathogenic fungus Pandora neoaphidis. Harmonia axyridis entirely consumes P. neoaphidis-sporulating cadavers and this may have a negative effect on the epizootic potential of P. neoaphidis. Here we assessed within plant transmission, and between plant vectoring, of P. neoaphidis in the presence of either H. axyridis or Coccinella septempunctata, a native coccinellid that only partially consumes fungal cadavers. Transmission was greater in the presence of coccinellids, with 21% of aphids becoming infected with the fungus whilst only 4% were infected in the control. However, there was no significant effect of coccinellid species or sex on fungal transmission. Between plant vectoring occurred infrequently in the presence of both species of coccinellid. The effect of H. axyridis on P. neoaphidis transmission is, therefore, likely to be similar to that of the native coccinellid C. septempunctata. and Patricia M. WELLS, Jason BAVERSTOCK, Michael E.N. MAJERUS, Francis M. JIGGINS, Helen E. ROY, Judith K. PELL.
Larvae of Hermetia illucens feed on different types of decomposing organic matter and their development depends on the quality and quantity of food ingested. In this study the effect of three artificial diets was analyzed, namely hen feed, meat meal and a mixture of these two diets. The effects of diet on ovarian development, size, mortality, duration of the larval and pupal stages and sex ratio were studied. Results indicate that the meat meal diet was the worst of the three diets in terms of percentage mortality and duration of the larval and pupal stages. We conclude that food ingested by the black soldier fly larvae determines both the physiological and morphological development of the adults., Paola Gobbi, Anabel Martínez-Sánchez, Santos Rojo., and Obsahuje seznam literatury
1_Coraebus florentinus (Herbst) is one of the most important wood borer pests of oaks in forest ecosystems in the Mediterranean Region. It is considered to be a heliophilous species as it prefers the sunniest parts of the canopy of isolated trees. The biological significance of this preference is still unknown. Recently, the effect of temperature on the preimaginal development of this insect was established: high temperatures increase its probability of survival and shorten its developmental time. Continuing this line of research, this study was designed to determine whether C. florentinus exhibits selective oviposition behaviour and how variation in temperature due to differences in the position of the branches in which the larvae develop could affect the subsequent development of this species. To determine whether this insect selects the branches in which to lay its eggs, location data (north, south, upper half and lower half of the tops of the trees) for 112 damaged branches were analysed. The results confirm that females of C. florentinus do not lay their eggs at random at the tops of trees but rather choose branches that are exposed to the sun. To determine the effects of larval rearing temperature on the later development stages, an experiment consisting of five treatments was performed., 2_Four of these treatments, each containing 25 infested branches derived from different orientations and positions in a tree (upper half of tree and north facing, upper half and south facing, lower half and north facing and lower half and south facing) were kept in culture chambers maintained at optimal conditions for pupal development (28 ± 2°C and 60–65% relative humidity). The fifth treatment with 25 branches infested collected from the most sun-exposed locations were kept in outdoor conditions. The results indicate that variation in temperature during larval development due to differences in branch location does not significantly affect survival, duration of developmental of pupae, emergence success or sex ratio of the adults., Ana M. Cárdenas, Patricia Gallardo., and Obsahuje seznam literatury
Five new species of Mulcticola Clay et Meinertzhagen, 1938 are described and illustrated from Brazil. These new species and their hosts are: Mulcticola sicki sp. n. from the sand-coloured nighthawk, Chordeiles rupestris rupestris (Spix), Mulcticola bacurau sp. n. from the common pauraque, Nyctidromus albicollis (Gmelin), Mulcticola tendeiroi sp. n. from the long-trained nightjar, Macropsalis forcipata (Nitzsch), Mulcticola piacentinii sp. n. from the short-tailed nighthawk, Lurocalis semitorquatus semitorquatus (Gmelin) (type-host) and L. s. nattereri (Temminck), and Mulcticola parvulus sp. n. from the little nightjar, Setopagis parvula (Gould). These species were compared primarily with Mulcticola nacunda Carriker, 1945 from the nacunda nighthawk, Chordeiles nacunda nacunda (Vieillot), which is one of the species of Mulcticola previously recorded in the Neotropical region. All the five new species described herein differ from their congeners by exclusive characters such as the shape of anterior dorsal head plate, metasternal plate, subvulvar plates in females and genitalia in males. We increased the number of species in Mulcticola to 18 in total, with seven of them now known from the Neotropics. We present the main morphological characters to distinguish Mulcticola from other species of the Philopteridae parasitising Caprimulgiformes and also compile a detailed catalogue for species included in this louse genus., Michel P. Valim, Kamila M. D. Kuabara., and Obsahuje bibliografii
Babesiosis is an emerging zoonotic disease and various wildlife species are reservoir hosts for zoonotic species of Babesia Starcovici, 1893. The objective of the present study was to investigate the presence and prevalence of Babesia spp. in moose Alces alces (Linnaeus) in two regions of Norway. A total of 99 spleen samples were collected from animals of various ages from an area with the occurrence of the tick Ixodes ricinus (Linnaeus, 1758), and from an area where the ticks are known to be absent. Infection was detected by the amplification of different regions of the 18S rRNA gene by using two different PCR primer sets specific of Babesia. Babesia spp. were found in the spleen samples of four moose. All Babesia-infected animals were from an area where ticks occur, with an infection rate of 6% (4 of 70). Babesia-positive samples were obtained from a five-month old moose calf and three adults. Two Babesia species, Babesia capreoli (Enigk et Friedhoff, 1962) and a B. odocoilei-like, were identified. Co-infection with Anaplasma phagocytophilum was obtained in two animals. This is the first report of the occurrence of B. capreoli and B. odocoilei-like species in moose., Irma Pūraitė, Olav Rosef, Jana Radzijevskaja, Indrė Lipatova, Algimantas Paulauskas., and Obsahuje bibliografii
The aim of this review is to explain the functional significance of mantis peering behaviour from an entomological perspective. First the morphological and optical features of the mantis compound eye that are important for spatial vision are described. The possibility that praying-mantises use binocular retinal disparity (stereopsis) and other alternative visual cues for determining distance in prey capture, are discussed. The primary focus of the review is the importance of peering movements for estimating the distance to stationary objects. Here the following aspects are examined: (1) Direct evidence via object manipulation experiments of absolute distance estimation with the aid of self-induced retinal image motion; (2) the mechanism of absolute distance estimation (with the interaction of visual and proprioceptive information); (3) the range of absolute and relative distance estimation; (4) the influence of target object features on distance estimation; and (5) the relationship between peering behaviour and habitat structures, based on results of studies on three species of mantis., Karl Kral., and Obsahuje seznam literatury
a1_The infraspecific taxonomy of the European populations of the Large Blue (Maculinea arion) is confusing. Several subspecies have been described mostly based on external morphological features. In the Carpathian Basin two subspecies have been distinguished. Maculinea arion arion flies from mid-May to mid-June and Maculinea arion ligurica is on the wing from the end of June to mid-August. The two forms show some differentiation in habitat use, but occasionally can also share habitats with two peaks in the appearance of butterflies. Our aim was to study the level and structure of genetic variation in a set of populations of the two phenologically different M. arion. Imagos were collected from 8 localities between 2000 and 2006. Enzyme polymorphism was analysed at 13 enzyme loci using polyacrylamide gel electrophoresis. In the analysis of the data, we estimated the parameters of polymorphism. To study the pattern of genetic differentiation F-statistics, hierarchical F-statistics and AMOVA were computed. GeneClass and Structure were both applied to analyse the differentiation between the two phenologically different sets of populations. Cavalli-Sforza and Edwards' arc distances were calculated and a UPGMA dendrogram was constructed on the basis of the distance matrix. PCA analysis was also carried out using the allele frequencies of the individuals. The level of polymorphism was relatively high in M. arion. The results of all analyses indicated that the differences between the two sets of phenologically different populations accounted for a low percentage of the total differentiation. In addition, a sizeable amount of variation could be attributed to the differences among the samples collected from the same population in consecutive years. Thus, we concluded that the "spring" and "summer arion" could not be considered as separate ESUs, although we could attribute conservation value to both forms on the basis of, a2_their phenological differentiation and habitat use., and Judit Bereczki, János P. Tóth, Andrea Tóth, Edit Bátori, Katalin Pecsenye, Zoltán Varga.
Endemic Sicilian pond turtles Emys trinacris Fritz, Fattizzo, Guicking, Tripepi, Pennisi, Lenk, Joger et Wink were examined for the presence of haemogregarine parasites. The presence of haemogregarines, occurring mainly in the microgametocyte stage (13.2 ± 0.12 μm in length and 6.4 ± 0.52 μm in width), was observed in approximately 9% of the sampled E. trinacris. Based on the observed morphology and on the sequencing of nuclear 18S rDNA, we identified the parasite as Haemogregarina stepanowi Danilewsky, 1885. Morphometric study of uninfected and infected red blood cells has shown that H. stepanowi induces different changes in erythrocyte shape depending on the infective stage. The differential count of leukocytes in specimens infected with H. stepanowi showed no significant difference compared with healthy specimens. However, considering the health problems which might be induced by H. stepanowi in the closely related European pond turtle Emys orbicularis (Linneaus), monitoring of the health status of the infected Sicilian populations of E. trinacris is desirable. The restricted distribution of populations of Emys infected with haemogregarines in Sicily is quite puzzling and the possible human-mediated introduction of the parasite in Sicily is briefly discussed., Vincenzo Arizza, Francesco Sacco, Debora Russo, Rita Scardino, Marco Arculeo, Melita Vamberger, Federico Marrone., and Obsahuje bibliografii
Several studies have investigated the association between infection with Toxoplasma gondii (Nicolle et Manceaux, 1908), pregnancy and fertility, but the results of studies focused on the fertility are rather ambiguous. Here we report results of four new cross-sectional studies. The studies were performed in the General University Hospital, Prague (study A with n = 1 165, and study C with n = 317), in private clinics of the Centre of Reproductive Medicine, Prague (study B with n = 1 016), and in a population of Czech and Slovak volunteers from the Facebook page 'Guinea Pigs' willing to participate in various basic science studies (study D with n = 524). In studies A and B, the clinical records were used to assess the fertility problems, whereas in studies C and D, the women were asked to rate their fertility problems using a six-point scale. Pregnant T. gondii-infected women were older than T. gondii-free women (study A: 33.1 vs 31.2, P < 0.001; study B: 30.6 vs 29.6, P = 0.012) and more often used assisted reproductive technology to conceive (study A: 17.2% vs 12.4%, P = 0.041; study B: 13.4% vs 9.2%, P = 0.317). Pregnant T. gondii-infected primiparous women were older than T. gondii-free primiparas (study A: 31.1 vs 29.5, P < 0.001; study B: 29.7 vs 28.9, P = 0.064) and more often used assisted reproductive technology to conceive (study A: 24.7% vs 14.4%, P = 0.010; study B: 15.9% vs 15.5%, P = 0.888). T. gondii-infected women reported to take a longer time to conceive than T. gondii-free women (P = 0.015). They also claimed to have more fertility problems than T. gondii-free women (P < 0.0001). Our results suggest that 'asymptomatic' latent toxoplasmosis could be a more serious source of fertility problems and health-associated burden than more severe but far rarer congenital toxoplasmosis., Šárka Kaňková, Jaroslav Flegr, Pavel Calda., and Obsahuje bibliografii
Operophthera brumata Linnaeus, 1758). Our calculations are based on data collected by the Hungarian Forestry Light Trap Network at four sites from 1961 to 2008. We also tried to estimate the influence of polarized moonlight and collecting distance, which also depends on moonlight. Our investigations revealed that the catches were the greatest in the First and the Last Quarters, and the lowest at Full Moon. The reason for this is that the proportion of polarized moonlight in the different lunar quarters varies, with the catches highest when the proportion is greatest. Collecting distance has only a minor role. and Obsahuje seznam literatury