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752. Filosofie XIX. století a vědy přírodní: (zvláštní otisk z "Noviny" ročník 5.)

Creator:
František Drtina
Publisher:
s.n.
Format:
print and 19 s.
Type:
model:monograph and TEXT
Subject:
Filozofické systémy a hlediska, 19. století, evropská filozofie, dějiny filozofie, přírodní vědy, dějiny přírodních věd, 14(4), 14(091), 5, 5(091), (0:82-4), and 14
Language:
Czech
Description:
napsal Fr. Drtina. and KČSN
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

753. Fitting a single-phase model to the post-exercise changes in heart rate and oxygen uptake

Creator:
Stupnicki, R., Gabryś, T., Szmatlan-Gabryś, U., and Tomaszewski, P.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, srdeční rytmus, heart rate, post-exercise recovery, oxygen uptake, half-recovery time, 14, and 612
Language:
English
Description:
The kinetics of post-exercise heart rate (HR) and oxygen consumption (EPOC) was studied in 10 elite cyclists subjected to four laboratory cycle ergometer maximal exercises lasting 30, 90, 180 or 360 s. Heart rate and oxygen uptake (VO2) were recorded over a period of 6 min after the exercise. By applying the logit transformation to the recorded variables and relating them to the decimal logarithm of the recovery time, uniform single-phase courses of changes were shown for both variables in all subjects and exercises. This enabled computing half-recovery times (t½) for both variables. Half-time for VO2 negatively correlated with square root of exercise duration (within-subject r=-0.629, p<0.001), the total post-exercise oxygen uptake till t½ was thus constant irrespectively of exercise intensity. The method is simple and enables reliable comparisons of various modes of exercise with respect to the rate of recovery., R. Stupnicki ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

754. Flow cytometric method for estimation of 5-bromo-2'deosyuridine content in rat serum

Creator:
Jaromír Mikeš, Ševc, J., Košuth, J., Matiašová, A., Zuzana Daxnerová, and Peter Fedoročko
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, proliferace buněk, průtoková cytometrie, cell proliferation, flow cytometry, BrdU pharmacokinetic, BrdU incorporation, HCT-116, 14, and 612
Language:
English
Description:
Labelling of DNA in replicating cells using 5-bromo-2'- deoxyuridine (BrdU) is widely used, however the rapid clearance and metabolisation of BrdU in the living organism is a critical issue. Although the pharmacokinetic of BrdU in experimental animals is empirically approximated, the exact time-curve remains unknown. Here we present novel method for estimation of the BrdU content in the blood serum. The application is based on the in vitro cocultivation of tumour cells with the examined serum and the subsequent quantification of the incorporated BrdU in the DNA using flow cytometry analysis. Our results demonstrate that this approach can quantify the BrdU concentration in serum at 1 μmol.dm-3 and might represent an attractive alternative to conventional chromatographic analysis. The employment of tumour cells as "detectors" of the BrdU content in serum provides an advantage over high pressure liquid chromatography (HPLC), as this approach allows us to approximate not only the concentration of BrdU, but also to determine, whether BrdU is present in the blood serum in effective concentration to reliable label all cells undergoing the S-phase of the cell cycle. The presented application might be a helpful tool for studies on pharmacokinetics of BrdU or other thymidine analogues when testing various administration routes or protocols., J. Mikeš, J. Ševc, J. Košuth, A. Matiašová, Z. Daxnerová, P. Fedoročko., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

755. Fluorescent advanced glycation end products in the detection of factual stages of cartilage degeneration

Creator:
Milan Handl, Elena Filová, Martin Kubala, Zdeněk Lánský, Koláčná, L., Vorlíček, J., Tomáš Trč, Miroslav Pach, and Evžen Amler
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Lékařské vědy. Lékařství, lékařská biofyzika, fluorescenční diagnostika, kolenní kloub, chrupavka, medical biophysics, fluoroscence diagnostics, knee joint, cartilage, neenzymová glykace, nonenzymic glycation, 14, and 61
Language:
English
Description:
Patients treated for knee disorders were included in this study. They were examined clinically (Lequesne and Tegner scores) and by standard X-ray investigation. Patients underwent a surgical procedure, either arthroscopy or knee replacement. At the initial phase of surgery, a sample of cartilage was taken for laboratory examination. Progression of the disorder and the clinical examination was correlated with the actual state of the cartilage using a novel fluorescence approach. The intrinsic fluorescence of cartilages was shown as a suitable and sensitive method for detection of the actual state of cartilages because the correlation with X-ray examination and clinical status was found. Intrinsic fluorescence properties of cartilages from patients with chondropathy and osteoarthritis were described and found to be age-dependent. We also observed a higher concentration of advanced glycation end products due to inflammatory and/or degenerative processes in the cartilage. In addition, acute pathological changes due to diseases such as meniscal lesions or anterior cruciate ligament rupture caused a significant increase of formation of advanced glycation end products even in the group of young patients. In fact, such an observation could be crucial and important for the detection of knee conditions suspected of early meniscal and/or ACL lesions especially among young patients., M. Handl, E. Filová, M. Kubala, Z. Lánský, L. Koláčná, J. Vorlíček, T. Trč, M. Pach, E. Amler., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

756. Fluoride Plus Aluminum: Useful Tools in Laboratory Investigations, but Messengers of False Information

Creator:
Anna Strunecká, Strunecký, O., and Patočka, J.
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Aluminum, Fluoride, Aluminofluoride complexes, G-protein, Second messenger, 14, and 612
Language:
English
Description:
Aluminofluoride complexes (AlFx) form spontaneously in aqueous solutions containing fluoride and traces of aluminum ions and appear to act as phosphate analogs. These complexes have become widely utilized in laboratory investigations of various guanine nucleotide-binding proteins. Reflecting on many laboratory studies, a new mechanism of fluoride and aluminum action on the cellular level is being suggested. The long-term synergistic effects of these ions in living environment and their hidden danger for human health are not yet fully recognized., A. Strunecká, O. Strunecký, J. Patočka., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

758. František Cyrill Kampelík: obraz životopisný

Creator:
František Komárek
Publisher:
tiskem a nákladem knihtiskárny Bratří Peřinů
Format:
print, text, regular print, and 63 s. ; 19 cm
Type:
text, volume, biography, biografie, model:monograph, and TEXT
Subject:
Patologie. Klinická medicína, Biografie, Kampelík, František Cyril, 1805-1872, 19. století, lékaři, Česko, 929, 616-051, (437.3), (092), 929 Kampelík, František Cyril, 14, 8, and 616
Language:
Czech
Description:
napsal František Komárek
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

759. Frequency-domain lifetime fluorometry of double-labeled creatine kinase

Creator:
Gregor, M., Martin Kubala, Evžen Amler, and Jiří Mejsnar
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, creatine kinase molecule, conformational change, FRET, frequency-domain lifetime fluorometry, 14, and 612
Language:
English
Description:
Myofibril-bound creatine kinase EC 2.7.3.2 (CK), a key enzyme of muscle energy metabolism, has been selected for studies of conformational changes that underlie the cellular control of enzyme activity. For fluorescence spectroscopy measurements, the CK molecule was double-labeled with IAF (5-iodoacetamidofluorescein) and ErITC (erythrosin 5'-isothiocyanate). Measurement of fluorescence resonance energy transfer (FRET) from fluorescein to erythrosin was used to obtain information about the donor-acceptor pair distance. Frequency-domain lifetime measurements evaluate the donor-acceptor distance in the native CK molecule as 7.8 nm. The Förster radius equals 5.3 nm with the resolution range from 0.2 to 1.0 nm. Erythrosin-fluorescein labeling (EFL) was tested for artificial conformational changes of the CK molecule with high-salt concentration treatment. The transition distance, defined by His-97 and Cys-283 and derived from a 3D model equals 0.766 nm for the open (inactive) form and 0.277 nm for the closed (reactive) form of the CK molecule. In this way, the resolution range of the used spectroscopy method is significant, concerning the difference of 0.489 nm. Nevertheless, the CK enzyme activity, assessed by the hexokinase-coupled assay, was diminished down to 1 % of the activity of the native enzyme. EFL is suitable for description of conformational behavior implied from the regulation of creatine kinase. However, the observed inhibition restricts EFL to studies of conformational changes during natural catalytic activity., M. Gregor, M. Kubala, E. Amler, J. Mejsnar., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

760. From the first spectral analysis of blood pressure variability in the world to the present time: contribution of the department of physiology of the faculty of medicine, masaryk university, brno

Creator:
Nováková, Z.
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, finger arterial blood pressure, blood pressure variability, baroreflex sensitivity, sudden cardiac death, hypertension, area under receiving operating curve, 14, and 612
Language:
English
Description:
The review shows the significance of blood pressure regulation studies conducted during several decades at the Department of Physiology, Faculty of Medicine, Masaryk University, Brno. Continuous non-invasive blood pressure measurement was first introduced and patented here and, with the obtained data, the first spectral analysis of blood pressure was performed. This method was used in many different physiological studies on the relationship of blood pressure regulation to circulatory parameters, breathing, and baroreflex sensitivity. The article deals with studies on risk stratification of sudden cardiac death according to decreased baroreflex sensitivity, 24-hour heart rate variability, the amount of extrasystoles and late potentials. Importance of the new method of determination of one summation risk index is described here. A summary of the new conception of the relationship between low baroreflex sensitivity and hypertension is presented. Here, not only pathological changes of the vessel wall but also increased sympathetic activity and genetic predisposition play a role. Importance of studies conducted in young adults is highlighted, as inherited BRS decrease contributes to earlier blood pressure increase in the young. This research is highly topical, since prevention of hypertension in childhood is possible. Recent studies are dedicated to blood pressure regulation in young diabetics., Z. Nováková., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

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