Článek se věnuje nově popsanému rodu Oreojuncus Záveská Drábková et Kirschner, který zahrnuje dva druhy - Oreojuncus trifidus a Oreojuncus monanthos. V České republice se vyskytuje pouze O. trifidus, sítina horská trojklanná, které je věnována větší pozornost. Rod Oreojuncus byl tradičně zařazován mezi sítiny (Juncus) sekce Steirochloa, avšak na základě komplexního výzkumu zahrnujícího fylogenetickou analýzu molekulárních dat a revizi morfologie byl vyčleněn jako samostatný, osmý rod čeledi sítinovitých (Juncaceae)., The new genus Oreojuncus Záveská Drábková et Kirschner has been described, with two species - O. trifidus and O. monanthos. Attention is given to the first species, which occurs in the Czech Republic. The genus Oreojuncus has been traditionally included in the Juncus sect. Steirochloa; however, complex research based on phylogenetic analysis of the molecular data and morphological revision revealed the necessity to establish this eighth genus in the Juncaceae family., and Lenka Záveská Drábková.
To investigate the role of glycine betaine in photosynthesis under stress, a transgenic wheat (Triticum aestivum L.) line T6 overaccumulating glycine betaine and its wild type Shi4185 were used. Seedlings were exposed to conditions of drought (30%, PEG-6000), heat (40°C) and their combination. The results revealed ultrastructural damage to the chloroplast and thylakoid lamellae with the withered phenotype by both drought and heat stress, and the damage was exacerbated by the combination of drought and heat. The appearance of a K step in the typical O-J-I-P curve and the decrease of Hill activity indicated a reduction of oxygen evolving complex function caused by stress. The greater damage was found in wild type than T6. Overaccumulation of glycine betaine in T6 could protect lipids in the thylakoid membrane from damage and stabilize the index of unsaturated fatty acids under stress. A lower ratio of monogalactosyl diacylglycerol/digalactosyl diacylglycerol and higher phosphatidylglycerol content in the thylakoid membrane of T6 were also observed under stress. These effects can promote stability of the thylakoid membrane. Otherwise, glycine betaine overaccumulation decreased photoinhibition of PSII under stress. The results also suggest that xanthophyll cycle-dependent non-radiative energy dissipation may be involved in the GB-mediated effects on PSII function under stress conditions. and G. P. Wang ... [et al.].
We investigated the different responses of wheat (Triticum aestivum L.) plants to drought- (DS) and heat stress (HS), and analyzed the physiological mechanisms of glycine betaine (GB) involved in the improvement of wheat tolerance to the combination of these stresses. The transgenic wheat T6 line was generated by introducing a gene encoding betaine aldehyde dehydrogenase (BADH) into the wild-type (WT) Shi4185 line. The gene was cloned from the Garden Orache plant (Atriplex hortensis L.). Wheat seedlings were subjected to drought stress (30%, PEG-6000), heat stress (40°C), and their combination. Photosynthetic gas exchange, water status and lipid peroxidation of wheat leaves were examined under different stresses. When subjected to a combination of drought and heat, the inhibition of photosynthesis was significantly increased compared to that under DS or HS alone. The increased inhibition of photosynthesis by the combined stresses was not simply the additive stress effect of separate heat- and drought treatments; different responses in plant physiology to DS and HS were also found. HS decreased the chlorophyll (Chl) content, net photosynthetic rate (PN), carboxylation efficiency (CE) and apparent quantum yield (AQY) more than DS but DS decreased the transpiration rate (E), stomata conductance (gs) and intercellular CO2 concentration (Ci) more than HS. GB over-accumulation led to increased photosynthesis not only under individual DS or HS but also under their combination. The enhancement of antioxidant activity and the improvement of water status may be the mechanisms underlying the improvement of photosynthesis by GB in wheat plants. and G. P. Wang ... [et al.].
Fifty years of botanical investigation in the region of Podblanicko (Central Bohemia) published in the regional collection Nature and History of Podblanicko Region.