Microplastics (particles of plastics <5 mm) affect the physical, biological and hydrological properties of agricultural soil, as well as crop growth. We investigated the effect of the addition of three microplastics (high-density polyethylene (HDPE), polyvinyl chloride (PVC), and polystyrene (PS)) at a concentration of 5% (w/w) to a silty loam soil on selected soil properties and growth of radish (Raphanus sativus L. var. sativus). Changes in the soil properties and radish growth in three microplastic treatments were compared with the control. Soil properties (bulk density, hydraulic conductivity, sorptivity, water repellency) were estimated for each treatment at the beginning and at the end of the radish growing period (GP). The bulk density was significantly lower in the HDPE and PVC treatments compared to the control within the measurement at the beginning of the GP and in all microplastic treatments compared to the control at the end of the GP. The values of hydraulic conductivity and water sorptivity did not show significant differences between any treatments within the measurement at the beginning of GP, but they were significantly higher in the HDPE treatment compared to the control at the end of the GP. The growth of radish was characterized by the plant biomass and effective quantum yield of Photosystem II (Y (II)). We did not find a statistically significant difference in the total biomass of radish between any of the experimental treatments, maybe due to used concentration of microplastics. The mean value of Y (II) was significantly higher in all microplastic treatments compared to control only within the last measurement at the end of the GP. A statistically significant change of Y(II) in all microplastic treatments may indicate functional shift in soil properties; however, the measured values of the soil characteristics have not shown the significant changes (except for the bulk density values in all microplastic treatments and hydraulic conductivity together with sorptivity in HDPE treatment within the measurement at the end of GP).