Ondrej Rochotský – Rochotius (1583 – after 1623) – was originally a Slovak humanist who worked on a permanent basis in Bohemia and Moravia. The presented study analyzes Rochotius’s theatrical school play Iosephiados comaedia, published in print in 1608 or 1609. I focus on analysing the characters in the play and exploring the ancient literary masterpieces that Rochotius used as sources of inspiration.