The paper discusses changes in the hydrological regime of high mountain Lake Morskie Oko located in the Tatra Mountains, in the Tatra Mountains National Park, a UNESCO biosphere reserve (MaB). According to the research conducted in the years 1971–2015, its water stages decreased by 3.5 cm·dec–1, mean annual water temperature increased by 0.3ºC·dec–1 and the duration of ice phenomena and ice cover was reduced by 10 day·dec–1. No considerable changes in maximum values of ice cover thickness were recorded. Such tendencies are primarily caused by long-term changes in climatic conditions – air temperature and atmospheric precipitation. The hydrological regime of the lake was also determined by changes in land use in the lake’s catchment and its location in high mountains.