The effect of photoautotrophy on photosynthesis and photoinhibition of gardenia plantlets during micropropagation

Title:

The effect of photoautotrophy on photosynthesis and photoinhibition of gardenia plantlets during micropropagation

Creator:

Serret, M. D., Trillas, M. I., Matas, J., and Araus, J. L.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0f0c2deb-889a-4d50-9991-a99b97d97503
uuid:0f0c2deb-889a-4d50-9991-a99b97d97503
issn:0300-3604
doi:10.1023/A:1013745007100

Subject:

chlorophyll fluorescence, Gardenia jasminoides, in vitro culture, irradiance, photosynthetic pigments, respiration, root induction, saccharose, shoot multiplication, and vapour pressure deficit

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We studied the relationships between the degree of photoautotrophy, photosynthetic capacity, and extent of photoinhibition of Gardenia jasminoides Ellis plantlets in vitro. Two successive micropropagation stages (shoot multiplication and root induction), and three culture conditions [tube cap closure, photosynthetic photon flux density (PPFD), and sucrose concentration] which may influence the development of photoautotrophy in vitro were assayed. The ratios of variable chlorophyll fluorescence to either maximal (Fv/Fm) or ground (Fv/F0) values were low, irrespective of the culture stage or growing conditions. Incomplete development of the photosynthetic apparatus and permanent photoinhibition may be involved. However, Fv/Fm and Fv/F0 increased from shoot multiplication to root induction owing to a decrease in F0 and an increase in Fm. This suggests that photoinhibition decreases later during micropropagation, when the photoautotrophy of plantlets is more advanced. The low sucrose content and high PPFD increased the photoinhibition of plantlets, whereas growth in tubes with permeable caps showed the opposite effect. The only culture factor with a significant (positive) effect on maximum photosynthetic rate (Pmax) was PPFD. At shoot multiplication net photosynthetic rate (PN) was positively correlated with the half time of the increase from F0 to Fm (t1/2). Such association may be mainly due to a common response of both traits to higher PPFD in culture. Within each culture stage, no relationship was observed between PN and the degree of photoautotrophy, which was positively correlated with Fv/Fm and Fv/F0 during root induction. During shoot multiplication, these correlations were not significant, or were even negative. Hence during the last stage of micropropagation, plantlets with a higher degree of photoautotrophy are less photoinhibited, whereas they do not follow this pattern at the earlier stage. and M. D. Serret ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

245-255

Source:

Photosynthetica | 2001 Volume:39 | Number:2

Harvested from:

CDK

Metadata only:

false