Monolayer film of phycobilisome-thylakoid membrane complexes from Spirulina platensis

Title:

Monolayer film of phycobilisome-thylakoid membrane complexes from Spirulina platensis

Creator:

Li, D.-H., Yang, S.-Z., Li, H., Xie, J., and Zhao, J.-Q.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1e4e3043-4637-4ed9-b66f-bd6f9dbf9039
uuid:1e4e3043-4637-4ed9-b66f-bd6f9dbf9039
issn:0300-3604
doi:10.1023/B:PHOT.0000046154.16834.0c

Subject:

absorption and fluorescence emission spectra, atomic force microscopy, cyanobacteria, Langmuir-Blodgett technique, and transmission electron microscopy

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Monolayer films of phycobilisome-thylakoid membrane complexes isolated from Spirulina platensis were prepared at air/aqueous solution interface by using the Langmuir-Blodgett technique. The film preparation was optimized with 0.5 M phosphate buffer (pH 7.0) as sub-phase at 20 °C. The monolayer was transferred into grids and into mica surface for observing the surface image of the complexes by transmission electron microscopy and atomic force microscope, respectively. The shape of complexes was disk-like with the diameter of about 50 nm and the thickness of about 35 nm. The absorption and fluorescence spectra of the complexes in the monolayer were consistent with those in buffer solution, which suggests that the complexes in the monolayer preserve the basic functional groups of photosynthetic apparatus and can be used as a model to investigate the structural connection and functional association of the light-harvesting antenna with the reaction centres. and D.-H. Li ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

365-370

Source:

Photosynthetica | 2004 Volume:42 | Number:3

Harvested from:

CDK

Metadata only:

false