Mechanical wounding caused by inoculation influences the photosynthetic response of Nicotiana benthamiana plants to plum pox potyvirus

Title:

Mechanical wounding caused by inoculation influences the photosynthetic response of Nicotiana benthamiana plants to plum pox potyvirus

Creator:

Hlaváčková, V., Špundová, M., Nauš, J., Navrátil, M., Kouřil, R., and Kaňa, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2f6182e7-845d-4307-a461-d86d4f453c15
uuid:2f6182e7-845d-4307-a461-d86d4f453c15
issn:0300-3604
doi:10.1023/A:1021358026974

Subject:

chlorophyll fluorescence, CO2 assimilation, ELISA-DASI, systemic response, net photosynthetic rate, thermoluminescence, tobacco, and viral infection

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Plants of Nicotiana benthamiana (Gray) (60 d old) were mechanically inoculated by a spreading of the fourth and fifth leaves with inoculum with or without plum pox potyvirus (PPV). Changes in growth parameters and selected photosynthetic characteristics were followed in control and inoculated plants in the locally affected leaves (LA) during 11 d after inoculation (DAI), in systemically affected leaves immature at time of inoculation (SAI) during 14-25 DAI, and in systemically affected leaves developed after the inoculation (SAD) during 28-39 DAI. The pure mechanical damage caused by inoculation induced a decrease in the net photosynthetic rate (PN) in LA and SAD leaves, and an increase in the steady-state value of the non-photochemical chlorophyll (Chl) fluorescence quenching qN. The qN increase appeared in certain time intervals in all measured leaves on plants, so it could be regarded as indication of a systemic reaction of plant to the local mechanical injury. The viral infection developed in LA leaves and spread to SAI and SAD leaves was documented by the ELISA-DASI method. The plant height and area of SAI and SAD leaves were lower in infected plants. The combined effect of mechanical damage and viral infection caused a decrease in PN only in LA and SAD leaves. In SAD leaves, an increased relative height of the J step (VJ) in the O-J-I-P Chl fluorescence transient together with a lower B/A band ratio of thermoluminescence glow curves reflected a damage to the acceptor side of photosystem 2 (PS2) caused by the viral infection, and a faster kinetics of the induction of the photochemical quenching coefficient qP of Chl fluorescence indicated a faster QA- re-oxidation in the remaining undamaged centres of PS2. and V. Hlaváčková ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

269-277

Source:

Photosynthetica | 2002 Volume:40 | Number:2

Harvested from:

CDK

Metadata only:

false