Mercury inhibits the activity of the NADPH:protochlorophyllide oxidoreductase (POR)

Title:

Mercury inhibits the activity of the NADPH:protochlorophyllide oxidoreductase (POR)

Creator:

Lenti, K., Fodor, F., and Böddi, B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:48eed037-2e76-4fba-b865-d1cf716b2828
uuid:48eed037-2e76-4fba-b865-d1cf716b2828
issn:0300-3604
doi:10.1023/A:1020143602973

Subject:

chlorophyll formation, fluorescence emission spectra, heavy metal, Hg, protochlorophyllide, Triticum, and wheat

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The effect of Hg++ was studied on the arrangement and photoactivity of NADPH:protochlorophyllide oxidoreductase (POR) in homogenates of dark-grown wheat (Triticum aestivum L.) leaves. 77 K fluorescence emission spectra of the homogenates were recorded before and after the irradiation of the homogenates and the spectra were deconvoluted into Gaussian components. The mercury treatment caused a precipitation of the membrane particles, which was followed by a remarkable decrease of the fluorescence yield. 10-3 M Hg++ decreased the ratio of the 655 nm-emitting protochlorophyllide (Pchlide) form to the 633 nm-emitting form. 10-2 M Hg++ shifted the short wavelength band to 629-630 nm and a 655 nm form was observed which was inactive on irradiation. This inhibition may be caused by serious alteration of the enzyme structure resulting in the trans-localisation of NADPH within the active site of POR. and K. Lenti, F. Fodor, B. Böddi.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

145-151

Source:

Photosynthetica | 2002 Volume:40 | Number:1

Harvested from:

CDK

Metadata only:

false