Effect of iron deficiency induced changes on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase, and photosystem activities in field grown grapevine (Vitis Vinifera L. cv. Pinot noir) leaves

Title:

Effect of iron deficiency induced changes on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase, and photosystem activities in field grown grapevine (Vitis Vinifera L. cv. Pinot noir) leaves

Creator:

Bertamini, M., Nedunchezhian, N., and Borghi, B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4babb229-4e18-471d-a158-5ff77d8271f0
uuid:4babb229-4e18-471d-a158-5ff77d8271f0
issn:0300-3604
doi:10.1023/A:1012491802184

Subject:

carotenoids, chlorophyll, fluorescence, electron transport, photosystems 1 and 2, proteins, and ribulose-1,5-bisphosphate carboxylase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The effect of iron deficiency on photosynthetic pigments, ribulose-1,5-bisphosphate carboxylase (RuBPC), and photosystem activities were investigated in field grown grapevine (Vitis vinifera L. cv. Pinot noir) leaves. The contents of chlorophyll (Chl) (a+b) and carotenoids per unit fresh mass showed a progressive decrease upon increase in iron deficiency. Similar results were also observed in content of total soluble proteins and RuBPC activity. The marked loss of large (55 kDa) and small (15 kDa) subunits of RuBPC was also observed in severely chlorotic leaves. However, when various photosynthetic electron transport activities were analysed in isolated thylakoids, a major decrease in the rate of whole chain (H2O → methyl viologen) electron transport was observed in iron deficient leaves. Such reduction was mainly due to the loss of photosystem 2 (PS2) activity. The same results were obtained when Fv/Fm was evaluated by Chl fluorescence measurements in leaves. Smaller inhibition of photosystem 1 (PS1) activity was also observed in both mild and severely chlorotic leaves. The artificial electron donors, diphenyl carbazide and NH2OH, markedly restored the loss of PS2 activity in severely chlorotic leaves. The marked loss of PS2 activity was evidently due to the loss of 33, 23, 28-25, and 17 kDa polypeptides in iron deficient leaves. and M. Bertamini, N. Nedunchezhian, B. Borghi.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

59-65

Source:

Photosynthetica | 2001 Volume:39 | Number:1

Harvested from:

CDK

Metadata only:

false