Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition

Title:

Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition

Creator:

Jiao, D. M., Li, X., and Ji, B. H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:652eb6c2-012b-4427-9ef4-84cedeed4185
uuid:652eb6c2-012b-4427-9ef4-84cedeed4185
issn:0300-3604
doi:10.1007/s11099-005-0082-2

Subject:

carbon assimilation, carbonic anhydrase, chlorophyll fluorescence, 3,3-dichloro-2-(dihydroxyphosphinoylmethyl)propenoate, enzymes scavenging active oxygen, malonyldialdehyde, Oryza, peroxidase, and superoxide dismutase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

With untransformed rice cv. Kitaake as control, the characteristics of carbon assimilation and photoprotection of a transgenic rice line over-expressing maize phosphoenolpyruvate carboxylase (PEPC) were investigated. The PEPC activity in untransformed rice was low, but the activity was stimulated under high irradiance or photoinhibitory condition. PEPC in untransformed rice contributed by about 5-10 % to photosynthesis, as shown by the application of the specific inhibitor 3,3-dichloro-2-(dihydroxyphosphinoylmethyl)propenoate (DCDP). When maize PEPC gene was introduced into rice, transgenic rice expressed high amount of maize PEPC protein and had high PEPC activity. Simultaneously, the activity of carbonic anhydrase (CA) transporting CO2 increased significantly. Thus the photosynthetic capacity increased greatly (50 %) under high CO2 supply. In CO2-free air, CO2 release in the leaf was less. In addition, PEPC transgenic rice was more tolerant to photoinhibition. Treating by NaF, an inhibitor of phosphatase, showed that in transgenic rice more phosphorylated light-harvesting chlorophyll a/b-binding complexes (LHC) moved to photosystem 1 (PS1) protecting thus PS2 from photo-damage. Simultaneously, the introduction of maize PEPC gene could activate or induce activities of the key enzymes scavenging active oxygen, such as superoxide dismutase (SOD) and peroxidase (POD). Hence higher PS2 photochemical efficiency and lower superoxygen anion (O2.-) generation and malonyldiadehyde (MDA) content under photoinhibition could improve protection from photo-oxidation. and D. M. Jiao, X. Li, B. H. Ji.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

501-508

Source:

Photosynthetica | 2005 Volume:43 | Number:4

Harvested from:

CDK

Metadata only:

false