Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants

Title:

Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants

Creator:

Pineda, M., Soukupová, J., Matouš, K., Nedbal, L., and Barón, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:68c64c08-d949-4309-a856-39a4c70593c7
uuid:68c64c08-d949-4309-a856-39a4c70593c7
issn:0300-3604
doi:10.1007/s11099-008-0076-y

Subject:

biotic stress, Nicotiana benthamiana, non-photochemical quenching, and Pepper mild mottle virus

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the last viral strain. and M. Pineda ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

441-451

Source:

Photosynthetica | 2008 Volume:46 | Number:3

Harvested from:

CDK

Metadata only:

false