Influence of the degree of solvent impurity on the spectrophotometric determination of chlorophylls in 80 % aqueous acetone and dimethyl formamide. Application to non-abrasive extraction of leaves of Citrus aurantium

Title:

Influence of the degree of solvent impurity on the spectrophotometric determination of chlorophylls in 80 % aqueous acetone and dimethyl formamide. Application to non-abrasive extraction of leaves of Citrus aurantium

Creator:

García, A. L. and Nicolás, N.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:68cb3464-a65f-4eec-a7dc-d1346d950879
uuid:68cb3464-a65f-4eec-a7dc-d1346d950879
issn:0300-3604
doi:10.1023/A:1006978907364

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Chlorophyll (Chl) a and Chl b absorbances were determined in extracts from leaves of Citrus aurantium L. using the solvents diethyl ether (100 %), buffered 80 % aqueous acetone, and N,N'-dimethyl formamide (DMF) (99.83 %). The respective standard deviations (σ) and coefficients of variability (CV) were used to establish the errors obtained when using impure solvents. Buffered 80 % aqueous acetone gave an error of 2.1 % for Chl a and 7.1 % for Chl b, while DMF gave errors of 3.5 and 6.7 %, respectively. For current Chl determination we propose a non-abrasive method, which extracts 15 leaf discs (diameter 6 mm) with 10 cm3 of buffered 80 % aqueous acetone or DMF in hermetically sealed flasks during 24 h in darkness at 4 °C. and A. L. García, N. Nicolás.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

545-550

Source:

Photosynthetica | 1998 Volume:35 | Number:4

Harvested from:

CDK

Metadata only:

false