Different mechanisms for photosystem 2 reversible down-regulation in pumpkin and soybean leaves under saturating irradiance

Title:

Different mechanisms for photosystem 2 reversible down-regulation in pumpkin and soybean leaves under saturating irradiance

Creator:

Zhang, Hai-Bo and Xu, Da-Quan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:715a6adc-fc43-48ad-bb19-831d099a4f8a
uuid:715a6adc-fc43-48ad-bb19-831d099a4f8a
issn:0300-3604
doi:10.1023/B:PHOT.0000011949.91626.ec

Subject:

chlorophyll fluorescence, Cucurbita, D1 protein, dissociation, Glycine, light-harvesting complex 2 (LHC2), polyacrylamide gel electrophoresis, species differences, and sucrose density gradient centrifugation

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

After saturating irradiation for 3 h (SI), the original fluorescence F0 increased while the photosystem 2 (PS2) photochemical efficiency (Fv/Fm) declined significantly. These parameters could largely recover to the levels of dark-adapted leaves after 3 h of subsequent dark recovery. No net loss of the D1 proteins occurred after SI. Soybean and pumpkin leaves had different responses to SI. Low temperature fluorescence parameters, F685 and F685/F735, decreased significantly in soybean leaves but not in pumpkin leaves. Part of the light-harvesting complex LHC2 dissociated from PS2 complexes in soybean leaves but not in pumpkin leaves, as shown by sucrose density gradient centrifugation and SDS-PAGE. The photon-saturated PS2 electron transport activity declined significantly in pumpkin thylakoids but not in soybean thylakoids. In addition, a large amount of phosphorylated D1 proteins was found in dark-adapted soybean leaves but not in dark-adapted pumpkin leaves. Hence at excessive irradiance soybean and pumpkin have the same protective strategy against photo-damage, reversible down-regulation of PS2, but two different mechanisms, namely the reversible down-regulation is related to the dissociation of LHC2 in soybean leaves but not in pumpkin leaves. and Hai-Bo Zhang, Da-Quan Xu.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

177-184

Source:

Photosynthetica | 2003 Volume:41 | Number:2

Harvested from:

CDK

Metadata only:

false