Effects of amino and thiol group reagents on the ferredoxin:NADP+ oxidoreductase catalysed reduction of dibromothymoquinone

Title:

Effects of amino and thiol group reagents on the ferredoxin:NADP+ oxidoreductase catalysed reduction of dibromothymoquinone

Creator:

Grzyb, J., Bojko, M., and Więckowski, S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7d379d7c-83ef-4535-b1c2-f3cb3cf8d4dd
uuid:7d379d7c-83ef-4535-b1c2-f3cb3cf8d4dd
issn:0300-3604
doi:10.1023/B:PHOT.0000027530.14195.52

Subject:

dichlorophenol indophenol, 5,5'-dithio-bis(2-nitrobenzoic) acid, fluorescamine, and Spinacia

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Effects of selective reagents of amino groups (fluorescamine, Fc) and thiol [5,5'-dithio-bis(2-nitrobenzoic) acid, DTNB] groups on the diaphorase activity of spinach ferredoxin:NADP+ oxidoreductase (FNR, E.C 1.18.1.2) in the presence of dibromothymoquinone (DBMIB) as an electron acceptor were studied. The incubation of FNR with 250 μM Fc in the time range from 0 to 120 min led to the gradual decrease of FNR activity according to biphasic kinetics. At the initial phase the activity (defined as the rate of NADPH oxidation) decreased about 4-time faster than at the subsequent second slower phase. Incubation of FNR simultaneously with Fc and DBMIB for more than 20 min caused restoration of the activity to about 80 % of the control. The inhibitory effect of Fc on the FNR-catalysed DBMIB reduction had non-competitive character. Incubation of FNR with DTNB led also to a gradual decrease of the enzyme activity, which reached about 45 % of the control after 2 h of incubation. Thus neither amino nor thiol groups in the FNR molecule are involved directly in the DBMIB reduction. However, the presence of DBMIB in the incubation medium influenced the inhibitory pattern of Fc and DTNB, and this suggests that DBMIB modified the conformational state of the FNR molecule. and J. Grzyb, M. Bojko, S. Więckowski.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

627-630

Source:

Photosynthetica | 2003 Volume:41 | Number:4

Harvested from:

CDK

Metadata only:

false