Isolation of chestnut chloroplasts: membrane potentials of chestnut and spinach thylakoids

Title:

Isolation of chestnut chloroplasts: membrane potentials of chestnut and spinach thylakoids

Creator:

Gomes-Laranjo, J., Salgado, P., Wong Fong Sang, H. W., Kraayenhof, R., and Torres-Pereira, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:81605ca7-b89a-451e-ac61-fcdb0ebf173e
uuid:81605ca7-b89a-451e-ac61-fcdb0ebf173e
issn:0300-3604
doi:10.1007/s11099-005-0039-5

Subject:

9-amino-6-chloro-2-methoxyacridine, Castanea sativa, fluorescence, osmotic potential, photosynthesis, Spinacia oleracea, and temperature

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Typical chestnut thylakoid extracts isolated by mechanical disruption of leaf tissues had an equivalent of 0.28 kg m-3 chlorophyll (Chl) which is six times less than in thylakoids obtained from spinach, although Chl content in leaves was only half as small. According to optical microscopy, the vesicles showed a good integrity, exhibiting at 21 °C a high capacity of photon-induced potential membrane generation, which was demonstrated by the almost full 9-amino-6-chloro-2-methoxyacridine fluorescence quenching in a hyper-saline medium containing 150 mM KCl and having osmotic potential of -1.5 MPa. The half-time of the thylakoid potential generation was 11.7 s with the time of dissipation around 8.9 s. In such conditions, spinach thylakoids showed an increased swelling and also differences in the half-time generation which was almost four times faster than was observed in chestnut. However, when spinach thylakoids were incubated in a typical hypo-saline medium without KCl with osmotic potential -0.8 MPa, no additional swelling was observed. Consequently the half-time of potential dissipation was 35 s. Studies with nigericin suggested a chestnut thylakoid ΔpH significantly smaller than that observed in spinach, which was confirmed by the measurements of the ATP driven pumping activity. and J. Gomes-Laranjo ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

237-246

Source:

Photosynthetica | 2005 Volume:43 | Number:2

Harvested from:

CDK

Metadata only:

false