Expression of vde gene integrated into tobacco genome in antisense and overexpressed ways

Title:

Expression of vde gene integrated into tobacco genome in antisense and overexpressed ways

Creator:

Deng, Ying, Lin, Rong-Cheng, Jing, Yu-Xiang, Wang, Qiang, Li, Liang-Bi, Liu, Bo-Lin, and Kuang, Ting-Yun

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9825c01f-5c96-4a0a-b90f-c58c6bd49693
uuid:9825c01f-5c96-4a0a-b90f-c58c6bd49693
issn:0300-3604
doi:10.1023/A:1025833118206

Subject:

Nicotiana, non-photochemical quenching, transgenic plants, violaxanthin de-epoxidase, and xanthophyll cycle

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Violaxanthin de-epoxidase (VDE) is localised in the thylakoid lumen of chloroplasts and catalyses de-epoxidation of violaxanthin into antheraxanthin and zeaxanthin. Tobacco vde gene was inserted into a binary vector pCAMBIA1301 with the hygromycin resistant gene for selection in antisense and overexpressed ways. Two constructs with antisense and overexpressed vde gene were introduced in tobacco (Nicotiana tabacum L.) using Agrobacterium tumefaciens strain LBA4404, PCR and Southern blot analyses demonstrated that the exogenous gene was integrated into genome of tobacco plants. VDE activity assay and HPLC analysis of pigments showed that the vde gene was expressed in the overexpressed transformants, whereas suppressed in the antisense ones. The chlorophyll fluorescence measurements proved that the contents of VDE in transgenic plants have a significant function in non-photochemical quenching. and Ying Deng ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

137-141

Source:

Photosynthetica | 2003 Volume:41 | Number:1

Harvested from:

CDK

Metadata only:

false