Analysis of photosystem II electron transfer with natural PsbA-variants by redox polymer/protein biophotoelectrochemistry

Title:

Analysis of photosystem II electron transfer with natural PsbA-variants by redox polymer/protein biophotoelectrochemistry

Creator:

Hartmann, V., Ruff, A., Schuhmann, W., Rögner, M., and Nowaczyk, M. M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9bb4d367-601d-44f9-aa71-7042cf110732
uuid:9bb4d367-601d-44f9-aa71-7042cf110732
issn:0300-3604
doi:10.1007/s11099-018-0775-y

Subject:

hydrogely, hydrogels, biophotovoltaics, cyanobacteria, D1-protein, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Redox polymer/protein biophotoelectrochemistry was used to analyse forward electron transfer of isolated PSII complexes with natural PsbA-variants. PsbA1- or PsbA3-PSII was embedded in a redox hydrogel that allows diffusion-free electron transfer to the electrode surface and thus measurement of an immediate photocurrent response. The initial photocurrent density of the electrode is up to ~2-fold higher with PsbA1-PSII under all tested light conditions, the most prominent under high-light [2,300 μmol(photon) m-2 s-1] illumination with 5 μA cm-2 for PsbA3-PSII and 9.5 μA cm-2 for PsbA1-PSII. This indicates more efficient electron transfer in low-light-adapted PsbA1-PSII. In contrast, the photocurrent decays faster in PsbA1-PSII under all tested light conditions, which suggests increased stability of high-light-adapted PsbA3-PSII. These results confirm and extend previous observations that PsbA3-PSII has increased P680+/QA- charge recombination and thus less efficient photon-to-charge conversion, whereas PsbA1-PSII is optimised for efficient electron transfer with limited stability., V. Hartmann, A. Ruff, W. Schuhmann, M. Rögner, M. M. Nowaczyk., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

229-235

Source:

Photosynthetica | 2018 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false