Structure-function correlation during the etioplast-chloroplast transition in cucumber cotyledonary leaves

Title:

Structure-function correlation during the etioplast-chloroplast transition in cucumber cotyledonary leaves

Creator:

Bala Krishna, K., Joshi, M. K., Vani, B., and Mohanty, P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a2f80ab7-7f89-44a4-93e7-41c45e9acd73
uuid:a2f80ab7-7f89-44a4-93e7-41c45e9acd73
issn:0300-3604
doi:10.1023/A:1007043409538

Subject:

chlorophyll fluorescence, intersystem pool size, light-harvesting complexes, photosystems 1 and 2, pigment-protein complexes, and reaction centre proteins

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We studied the development of chloroplasts from etioplasts in the cotyledonary leaves of 4-d-old dark-grown cucumber (Cucumis sativus) seedlings after irradiation (20 μmol m-2 s-1). Upon irradiation, the triggering of chlorophyll (Chl) synthesis and accumulation showed a relatively short lag phase. The irradiation of etiolated seedlings initiated the synthesis of apoproteins of pigment-protein complexes. While Chl-protein 2 (CP2) was detected at 6 h after irradiation, CP1 only after 29 h. The appearance and accumulation of some of the apoproteins were monitored by Western-blotting. LHC2 apoprotein was detected after a 6 h-irradiation. The amounts of D1 protein of photosystem (PS) 2 and PsaA/B protein of PS1 were quantitated by ELISA. Further, the thylakoid membrane function during this time period in terms of PS1- and PS2-mediated electron transfer activity and intersystem electron pool size were analyzed. While PS1 activity was detected after 4 h, PS2-mediated O2 evolution was detected only after a 17 h-irradiation. Fv/Fm value of Chl a fluorescence measurements indicated that the photochemical efficiency of these leaves reached its maximum after 29 h of irradiation. The intersystem pool size of cotyledonary leaves was equivalent to that of the control cotyledonary leaves grown for 25 h under continuous irradiation. Thus etioplasts develop into fully functional chloroplasts after approximately 25 h when 4 d-dark grown cucumber seedlings are continuously moderately irradiated. The development of photosynthetic electron transport chain seems to be limited in time at the level of PS2, possibly at the donor side. and K. Bala Krishna ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

199-212

Source:

Photosynthetica | 1999 Volume:36 | Number:1-2

Harvested from:

CDK

Metadata only:

false